LOCUS       BBa_K902086             1255 bp    DNA     linear       01-Jun-2024
DEFINITION  r0040-moco-moco-s7-j04500
ACCESSION   BBa_K902086
VERSION     BBa_K902086.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     promoter        63..191
                     /label=BBa_K902023
     promoter        200..328
                     /label=BBa_K902023
     misc_feature    646..646
                     /label=See Design Considerations
     CDS             335..1027
                     /label=BBa_K902019
     CDS             1036..1123
                     /label=end of LacI coding region (inactive)
     misc_feature    1036..1235
                     /label=BBa_R0010
     misc_feature    1124..1161
                     /label=CAP binding site
     promoter        1172..1177
                     /label=-35
     promoter        1196..1201
                     /label=-10
     misc_feature    1201..1235
                     /label=LacI binding site
     misc_feature    1208..1208
                     /label=start
     promoter        1036..1235
                     /label=BBa_R0010
     misc_feature    1248..1251
                     /label=conserved
     RBS             1244..1255
                     /label=BBa_B0034
     misc_feature    1036..1255
                     /label=BBa_J04500
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggggaacca ctaaacacct agcctctgca cctgggtcaa ctgatacggt gctttggccg
      121 tgacaatgct cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat
      181 ctggaaaggt gtactagagg ggaaccacta aacacctagc ctctgcacct gggtcaactg
      241 atacggtgct ttggccgtga caatgctcgt aaagattgcc accagggcga aggaagaaat
      301 gacttcgcct cccgtatctg gaaaggtgta ctagatgaca gagtatctcc taagcgcggg
      361 catctgtatg gccattgttt cgatcttatt gatcggcatg gcgatatcga acgtatctaa
      421 gggccagtac gcgaaacgct tcttcttttt cgccacgagt tgccttgtct taacattagt
      481 tgttgtttct tcactgtcgt ccagtgcgaa cgcctctcaa accgataacg gtgtaaatcg
      541 cagcgggagc gaagatccta ccgtgtattc cgcgacctca acgaaaaaac ttcataagga
      601 gcccgctacg ctcatcaaag ccattgacgg agacactgta aagctgatgt acaaaggcca
      661 accgatgacc tttcgcttgc tgctggtcga caccccagag acgaaacatc cgaaaaaggg
      721 cgtcgagaaa tacggcccgg aagcaagtgc gtttaccaag aagatggtgg aaaatgcgaa
      781 caaaattgaa gtcgaatttg ataaagggca gcgtaccgac aaatatggtc gtggactggc
      841 ctacatttat gctgatggta aaatggtgaa tgaagcactg gtgcggcagg gtctggccaa
      901 agtggcttat gtgtataaac cgaataatac tcacgaacag ctgctgcgta aatccgaagc
      961 acaggcaaaa aaagaaaaac tgaacatttg gagcgaagat aacgcggata gcggtcaata
     1021 ataataatac tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1081 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1141 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1201 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaa
//