##gff-version 3
##sequence-region BBa_K902086 1 1255
BBa_K902086	.	CDS	335	1027	.	+	0	ID=BBa_K902019;Name=BBa_K902019
BBa_K902086	.	sequence_alteration	646	646	.	+	0	ID=annotation2211172;Name=See Design Considerations;Parent=BBa_K902019
BBa_K902086	.	promoter	63	191	.	+	0	ID=BBa_K902023;Name=BBa_K902023
BBa_K902086	.	promoter	200	328	.	+	0	ID=BBa_K902023;Name=BBa_K902023
BBa_K902086	.	engineered_region	1036	1255	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_K902086	.	ribosome_entry_site	1244	1255	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K902086	.	conserved_region	1248	1251	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K902086	.	promoter	1036	1235	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K902086	.	start_codon	1208	1208	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K902086	.	CDS	1036	1123	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K902086	.	promoter	1196	1201	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K902086	.	non_covalent_binding_site	1201	1235	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K902086	.	non_covalent_binding_site	1124	1161	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K902086	.	engineered_region	1036	1235	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K902086	.	promoter	1172	1177	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K902086	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K902086	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K902086	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K902086	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K902086	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K902086	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K902086
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggggaaccactaaacacc
tagcctctgcacctgggtcaactgatacggtgctttggccgtgacaatgctcgtaaagattgccaccagggcgaaggaa
gaaatgacttcgcctcccgtatctggaaaggtgtactagaggggaaccactaaacacctagcctctgcacctgggtcaa
ctgatacggtgctttggccgtgacaatgctcgtaaagattgccaccagggcgaaggaagaaatgacttcgcctcccgta
tctggaaaggtgtactagatgacagagtatctcctaagcgcgggcatctgtatggccattgtttcgatcttattgatcg
gcatggcgatatcgaacgtatctaagggccagtacgcgaaacgcttcttctttttcgccacgagttgccttgtcttaac
attagttgttgtttcttcactgtcgtccagtgcgaacgcctctcaaaccgataacggtgtaaatcgcagcgggagcgaa
gatcctaccgtgtattccgcgacctcaacgaaaaaacttcataaggagcccgctacgctcatcaaagccattgacggag
acactgtaaagctgatgtacaaaggccaaccgatgacctttcgcttgctgctggtcgacaccccagagacgaaacatcc
gaaaaagggcgtcgagaaatacggcccggaagcaagtgcgtttaccaagaagatggtggaaaatgcgaacaaaattgaa
gtcgaatttgataaagggcagcgtaccgacaaatatggtcgtggactggcctacatttatgctgatggtaaaatggtga
atgaagcactggtgcggcagggtctggccaaagtggcttatgtgtataaaccgaataatactcacgaacagctgctgcg
taaatccgaagcacaggcaaaaaaagaaaaactgaacatttggagcgaagataacgcggatagcggtcaataataataa
tactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgac
tggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgc
ttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa