BBa_K906000 1 BBa_K906000 Zeaxanthin 7,8-cleavage dioxygenase (ZCD) 2012-07-29T11:00:00Z 2015-05-08T01:13:44Z This enzyme was first identified in Crocus sativus, the saffron plant. The originally identified gene can be found here [http://www.ncbi.nlm.nih.gov/nuccore/AJ489276.1 AJ489276.1] This is the enzyme required to cleave zeaxanthin at the 7, 8 position to begin the enzymatic process of making crocin, picrocrocin and safranal. ZCD seems to be unique to the species Crocus sativus, and has been found to be highly expressed in the style branch tissues of this species. Also, it has been shown to produce insoluble inclusion bodies when over expressed in E. coli. As a result, the functional expression of this protein requires the use of a soluble fusion proteins, such as thioredoxin, and induction at 20degrees. false false _1171_ 0 12629 9 In stock false This modified version of ZCD has been codon optimized for the species Synechocystis 6803. false Lucas B. Harrington annotation2179290 1 Stop range2179290 1 1108 1110 annotation2179289 1 Start range2179289 1 1 3 BBa_K906000_sequence 1 atgcaagtagaccccacaaaaggaatcggattagccaacacctctcttcagtttagtaatggccgcctgcatgccttgtgcgaatatgatctaccttacgtggtacggctgagtccggaagatggcgatatcagtaccgtgggtcgtatcgaaaacaatgtgtctaccaaaagtactaccgcacatcctaaaactgaccctgtgacgggagaaacctttagcttttcctacgggcccattcaaccctacgttacgtatagcagatatgattgcgacggcaaaaagagtggccccgatgttccgatattctcctttaaagaaccctctttcgtacatgactttgcaattactgagcactatgccgtgtttcctgatattcagattgtcatgaaaccagctgagattgttcggggtcggagaatgatcgggccagatttggaaaaagtcccccgtctggggctattaccgcgttatgccacctcagacagcgaaatgcgctggtttgacgtacccggttttaacatggtacacgtagtcaatgcgtgggaagaagagggaggcgaagtggtggtcattgttgccccgaatgtttcacccattgagaatgccattgatcgctttgatttgttacatgtgtccgtggagatggcgcggattgaactcaaatccggttccgtgagtcgcaccctactgtctgcggaaaacttagattttggagttattcatcggggttattctggtcgaaaaagcaggtacgcttacttgggcgtgggggatccaatgcccaaaattcgcggtgttgtgaaagtggacttcgaactggccggtcgaggggagtgtgttgtggctcggcgcgagtttggcgtcggctgtttcggaggggaacctttctttgtacccgcttccagcaagaagtcgggtggcgaagaagatgatggctatgttgtgagttacctccacgatgaaggcaagggtgaaagttcctttgtcgttatggacgcccgttcgccagaacttgaaatcttggctgaggtggtgttacccaggcgtgtcccctatgggtttcatgggttatttgtgacagaagcggaactcttgtcccaacaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z