BBa_K906003 1 BBa_K906003 Histidine Patch Thioredoxins (HP-Thioredoxin) 2012-07-29T11:00:00Z 2015-05-08T01:13:44Z This modified thioredoxin was produced by Zhijian Lu and collegues as described in the paper titled "Histidine Patch Thioredoxins MUTANT FORMS OF THIOREDOXIN WITH METAL CHELATING AFFINITY THAT PROVIDE FOR CONVENIENT PURIFICATIONS OF THIOREDOXIN FUSION PROTEINS" published in 1996 in the Journal of Biological Chemistry. A link to this paper can be found here : [http://www.jbc.org/content/271/9/5059.full] This is a mutant of E. coli's native thioredoxin. It has been modified to have the ability to chelate metal ions, notably nickel. This protein is useful as a fusion protein which can help to increase the solubility of difficult-to-express proteins and maximize yield of those proteins. false false _1171_ 0 12629 9 Not in stock false When used as a fusion protein, this protein is often combined with a inducible promoter such as pBAD/araC. false Lucas B. Harrington annotation2179293 1 Start range2179293 1 1 3 BBa_K906003_sequence 1 atgggatctgataaaattattcatctgactgatgattcttttgatactgatgtacttaaggcagatggtgcaatcctggttgatttctgggcacactggtgcggtccgtgcaaaatgatcgctccgattctggatgaaatcgctgacgaatatcagggcaaactgaccgttgcaaaactgaacatcgatcacaacccgggcactgcgccgaaatatggcatccgtggtatcccgactctgctgctgttcaaaaacggtgaagtggcggcaaccaaagtgggtgcactgtctaaaggtcagttgaaagagttcctcgacgctaacctggccggc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z