BBa_K906000 1 BBa_K906000 Zeaxanthin 7,8-cleavage dioxygenase (ZCD) 2012-07-29T11:00:00Z 2015-05-08T01:13:44Z This enzyme was first identified in Crocus sativus, the saffron plant. The originally identified gene can be found here [http://www.ncbi.nlm.nih.gov/nuccore/AJ489276.1 AJ489276.1] This is the enzyme required to cleave zeaxanthin at the 7, 8 position to begin the enzymatic process of making crocin, picrocrocin and safranal. ZCD seems to be unique to the species Crocus sativus, and has been found to be highly expressed in the style branch tissues of this species. Also, it has been shown to produce insoluble inclusion bodies when over expressed in E. coli. As a result, the functional expression of this protein requires the use of a soluble fusion proteins, such as thioredoxin, and induction at 20degrees. false false _1171_ 0 12629 9 In stock false This modified version of ZCD has been codon optimized for the species Synechocystis 6803. false Lucas B. Harrington annotation2179290 1 Stop range2179290 1 1108 1110 annotation2179289 1 Start range2179289 1 1 3 BBa_K906006 1 BBa_K906006 Extended psbA2 for Synechocystis 2012-08-12T11:00:00Z 2015-05-08T01:13:44Z This promoter comes from the native sequence of the region upstream of photosystem II D1 protein in Synechocystis spp. 6803. The sequences was synthsized by DNA 2.0 This is a promoter which has an extended 5' UTR relative to the previous biobrick submission of this promoter. The 5' UTR was exteneded because the previous version of this promoter has not been verified to work in Synechocystis spp. 6803, only in E. coli. Because less is known regarding the importance of false false _1171_ 0 12629 9 Not in stock false We added an additional 113 bases onto the previous biobrick version of psbA2 in order to increase the likleyhood of it functioning in Synechocystis spp. 6803. false Lucas B. Harrington annotation2179512 1 A ->T range2179512 1 200 200 BBa_K906100 1 BBa_K906100 Extended psbA2 with Zeaxanthin 7,8-cleavage dioxygenase (ZCD) 2012-08-12T11:00:00Z 2015-05-08T01:13:44Z psbA2 is from the genomic sequence preceding the photosystem II D1 protein coding region in Synechocystis spp. 6803. The modified version of ZCD has been codon optimized for the species Synechocystis 6803. This translational unit is designed to constitutivly express Zeaxanthin 7,8-cleavage dioxygenase (ZCD) in synechocystis. PsbA2 can be removed from this part and utilized with an alternative part by using NdeI to cut between the end of psbA2 and the start codon of ZCD. The ORF to be attached to this promoter should be made to have the same restriction enzyme site (possibly added by PCR) at its 5' end. This will allow for spacing between the regulatory features of psbA2 and the ORF to parallel that of the native use of the promoter. false false _1171_ 0 12629 9 It's complicated false The point mutation (A->T) enables the use of NdeI as mentioned on the main page. false Lucas B. Harrington component2180503 1 BBa_K906000 component2180500 1 BBa_K906006 annotation2180503 1 BBa_K906000 range2180503 1 201 1310 annotation2180500 1 BBa_K906006 range2180500 1 1 200 BBa_K906000_sequence 1 atgcaagtagaccccacaaaaggaatcggattagccaacacctctcttcagtttagtaatggccgcctgcatgccttgtgcgaatatgatctaccttacgtggtacggctgagtccggaagatggcgatatcagtaccgtgggtcgtatcgaaaacaatgtgtctaccaaaagtactaccgcacatcctaaaactgaccctgtgacgggagaaacctttagcttttcctacgggcccattcaaccctacgttacgtatagcagatatgattgcgacggcaaaaagagtggccccgatgttccgatattctcctttaaagaaccctctttcgtacatgactttgcaattactgagcactatgccgtgtttcctgatattcagattgtcatgaaaccagctgagattgttcggggtcggagaatgatcgggccagatttggaaaaagtcccccgtctggggctattaccgcgttatgccacctcagacagcgaaatgcgctggtttgacgtacccggttttaacatggtacacgtagtcaatgcgtgggaagaagagggaggcgaagtggtggtcattgttgccccgaatgtttcacccattgagaatgccattgatcgctttgatttgttacatgtgtccgtggagatggcgcggattgaactcaaatccggttccgtgagtcgcaccctactgtctgcggaaaacttagattttggagttattcatcggggttattctggtcgaaaaagcaggtacgcttacttgggcgtgggggatccaatgcccaaaattcgcggtgttgtgaaagtggacttcgaactggccggtcgaggggagtgtgttgtggctcggcgcgagtttggcgtcggctgtttcggaggggaacctttctttgtacccgcttccagcaagaagtcgggtggcgaagaagatgatggctatgttgtgagttacctccacgatgaaggcaagggtgaaagttcctttgtcgttatggacgcccgttcgccagaacttgaaatcttggctgaggtggtgttacccaggcgtgtcccctatgggtttcatgggttatttgtgacagaagcggaactcttgtcccaacaataa BBa_K906100_sequence 1 ctcttctcaacccccaaaacgccctctgtttacccatggaaaaaacgacaattacaagaaagtaaaacttatgtcatctataagcttcgtgtatattaacttcctgttacaaagctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccatatgcaagtagaccccacaaaaggaatcggattagccaacacctctcttcagtttagtaatggccgcctgcatgccttgtgcgaatatgatctaccttacgtggtacggctgagtccggaagatggcgatatcagtaccgtgggtcgtatcgaaaacaatgtgtctaccaaaagtactaccgcacatcctaaaactgaccctgtgacgggagaaacctttagcttttcctacgggcccattcaaccctacgttacgtatagcagatatgattgcgacggcaaaaagagtggccccgatgttccgatattctcctttaaagaaccctctttcgtacatgactttgcaattactgagcactatgccgtgtttcctgatattcagattgtcatgaaaccagctgagattgttcggggtcggagaatgatcgggccagatttggaaaaagtcccccgtctggggctattaccgcgttatgccacctcagacagcgaaatgcgctggtttgacgtacccggttttaacatggtacacgtagtcaatgcgtgggaagaagagggaggcgaagtggtggtcattgttgccccgaatgtttcacccattgagaatgccattgatcgctttgatttgttacatgtgtccgtggagatggcgcggattgaactcaaatccggttccgtgagtcgcaccctactgtctgcggaaaacttagattttggagttattcatcggggttattctggtcgaaaaagcaggtacgcttacttgggcgtgggggatccaatgcccaaaattcgcggtgttgtgaaagtggacttcgaactggccggtcgaggggagtgtgttgtggctcggcgcgagtttggcgtcggctgtttcggaggggaacctttctttgtacccgcttccagcaagaagtcgggtggcgaagaagatgatggctatgttgtgagttacctccacgatgaaggcaagggtgaaagttcctttgtcgttatggacgcccgttcgccagaacttgaaatcttggctgaggtggtgttacccaggcgtgtcccctatgggtttcatgggttatttgtgacagaagcggaactcttgtcccaacaataa BBa_K906006_sequence 1 ctcttctcaacccccaaaacgccctctgtttacccatggaaaaaacgacaattacaagaaagtaaaacttatgtcatctataagcttcgtgtatattaacttcctgttacaaagctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z