BBa_K906007 1 BBa_K906007 pBAD promoter 2012-08-21T11:00:00Z 2015-05-08T01:13:44Z This part comes from the thiofusion vector from Invitrogen. This is a version of the pBAD promoter which is repressed by the presence of araC and glucose. It is induced by the presence of L-arabanose at about 0.02%. false false _1171_ 0 12629 9 Not in stock false This part contains a NcoI restriciton site between the promoter and the Thioredoxin in the submitted part. false Lucas B. Harrington BBa_K906004 1 BBa_K906004 C-Terminal V5 Epitope and Polyhistidine Tag 2012-07-30T11:00:00Z 2015-05-08T01:13:44Z This part is a component of the vector included with the Invitrogen pBAD/Thio-TOPO kit. This is a tag which is added to the C terminus of a protein in order to aid in the identification of the protein in vivo, as well as to simplify the purificaiton process of the protein. The V5 epitope bind the Anti-V5 antibody. The histidine tag allows for purification using a nickel column. It was sourced from the Invitrogen pBAD/Thio-TOPO, which enables the expression of PCR amplified DNA. This protein also includes a linker to attach the V5 and histidine regions with the protein that is being expressed. false false _1171_ 0 12629 9 Not in stock false The sequence includes a stop codon (TGA) making it useful as a C-terminal tag. false Lucas B. Harrington annotation2179308 1 V5 Epitope range2179308 1 15 57 annotation2179310 1 Stop range2179310 1 85 87 annotation2179309 1 Hist-tag range2179309 1 69 84 BBa_K906102 1 BBa_K906102 ZCD with Histidine Patch Thioredoxins and Hist-tag 2012-08-21T11:00:00Z 2015-05-08T01:13:44Z ZCD originated from Crocus Sativus, the saffron plant. However the version present here has been codon optimized for synechocystis. This is the functional unit which makes ZCD soluble in E. coli. It includes a inducible promoter, mutated thioredoxin to increase solubility, the ZCD ORF, a hist-tag for easy purification and finally a double terminator. This part has been shown to highly express ZCD in E. coli. It is suggested that this part be used in a plasmid also containing araC for the optimal repression of this protein before the time of induction. false false _1171_ 0 12629 9 It's complicated true This part was codon optimized for Synechocystis, reflecting its planned expression in this cyanobacteria. false Lucas B. Harrington component2211310 1 BBa_K906000 component2211307 1 BBa_K906003 component2211314 1 BBa_K906004 component2211305 1 BBa_K906007 component2211315 1 BBa_K906005 annotation2211314 1 BBa_K906004 range2211314 1 1789 1875 annotation2211310 1 BBa_K906000 range2211310 1 679 1788 annotation2211307 1 BBa_K906003 range2211307 1 346 678 annotation2211305 1 BBa_K906007 range2211305 1 1 345 annotation2211315 1 BBa_K906005 range2211315 1 1876 2135 BBa_K906000 1 BBa_K906000 Zeaxanthin 7,8-cleavage dioxygenase (ZCD) 2012-07-29T11:00:00Z 2015-05-08T01:13:44Z This enzyme was first identified in Crocus sativus, the saffron plant. The originally identified gene can be found here [http://www.ncbi.nlm.nih.gov/nuccore/AJ489276.1 AJ489276.1] This is the enzyme required to cleave zeaxanthin at the 7, 8 position to begin the enzymatic process of making crocin, picrocrocin and safranal. ZCD seems to be unique to the species Crocus sativus, and has been found to be highly expressed in the style branch tissues of this species. Also, it has been shown to produce insoluble inclusion bodies when over expressed in E. coli. As a result, the functional expression of this protein requires the use of a soluble fusion proteins, such as thioredoxin, and induction at 20degrees. false false _1171_ 0 12629 9 In stock false This modified version of ZCD has been codon optimized for the species Synechocystis 6803. false Lucas B. Harrington annotation2179290 1 Stop range2179290 1 1108 1110 annotation2179289 1 Start range2179289 1 1 3 BBa_K906005 1 BBa_K906005 rrnB T1 and T2 2012-08-02T11:00:00Z 2015-05-08T01:13:44Z This is the terminator included as part of the ThioFusion Expression Kit made by invitrogen. This is a strong transctiptional terminator. It contains both a T1 and T2. false false _1171_ 0 12629 9 Not in stock false The part contains an upstream region containing a pBAD reverse priming site for amplification of the tagged protein without the terminator attached. false Lucas B. Harrington BBa_K906003 1 BBa_K906003 Histidine Patch Thioredoxins (HP-Thioredoxin) 2012-07-29T11:00:00Z 2015-05-08T01:13:44Z This modified thioredoxin was produced by Zhijian Lu and collegues as described in the paper titled "Histidine Patch Thioredoxins MUTANT FORMS OF THIOREDOXIN WITH METAL CHELATING AFFINITY THAT PROVIDE FOR CONVENIENT PURIFICATIONS OF THIOREDOXIN FUSION PROTEINS" published in 1996 in the Journal of Biological Chemistry. A link to this paper can be found here : [http://www.jbc.org/content/271/9/5059.full] This is a mutant of E. coli's native thioredoxin. It has been modified to have the ability to chelate metal ions, notably nickel. This protein is useful as a fusion protein which can help to increase the solubility of difficult-to-express proteins and maximize yield of those proteins. false false _1171_ 0 12629 9 Not in stock false When used as a fusion protein, this protein is often combined with a inducible promoter such as pBAD/araC. false Lucas B. Harrington annotation2179293 1 Start range2179293 1 1 3 BBa_K906000_sequence 1 atgcaagtagaccccacaaaaggaatcggattagccaacacctctcttcagtttagtaatggccgcctgcatgccttgtgcgaatatgatctaccttacgtggtacggctgagtccggaagatggcgatatcagtaccgtgggtcgtatcgaaaacaatgtgtctaccaaaagtactaccgcacatcctaaaactgaccctgtgacgggagaaacctttagcttttcctacgggcccattcaaccctacgttacgtatagcagatatgattgcgacggcaaaaagagtggccccgatgttccgatattctcctttaaagaaccctctttcgtacatgactttgcaattactgagcactatgccgtgtttcctgatattcagattgtcatgaaaccagctgagattgttcggggtcggagaatgatcgggccagatttggaaaaagtcccccgtctggggctattaccgcgttatgccacctcagacagcgaaatgcgctggtttgacgtacccggttttaacatggtacacgtagtcaatgcgtgggaagaagagggaggcgaagtggtggtcattgttgccccgaatgtttcacccattgagaatgccattgatcgctttgatttgttacatgtgtccgtggagatggcgcggattgaactcaaatccggttccgtgagtcgcaccctactgtctgcggaaaacttagattttggagttattcatcggggttattctggtcgaaaaagcaggtacgcttacttgggcgtgggggatccaatgcccaaaattcgcggtgttgtgaaagtggacttcgaactggccggtcgaggggagtgtgttgtggctcggcgcgagtttggcgtcggctgtttcggaggggaacctttctttgtacccgcttccagcaagaagtcgggtggcgaagaagatgatggctatgttgtgagttacctccacgatgaaggcaagggtgaaagttcctttgtcgttatggacgcccgttcgccagaacttgaaatcttggctgaggtggtgttacccaggcgtgtcccctatgggtttcatgggttatttgtgacagaagcggaactcttgtcccaacaataa BBa_K906003_sequence 1 atgggatctgataaaattattcatctgactgatgattcttttgatactgatgtacttaaggcagatggtgcaatcctggttgatttctgggcacactggtgcggtccgtgcaaaatgatcgctccgattctggatgaaatcgctgacgaatatcagggcaaactgaccgttgcaaaactgaacatcgatcacaacccgggcactgcgccgaaatatggcatccgtggtatcccgactctgctgctgttcaaaaacggtgaagtggcggcaaccaaagtgggtgcactgtctaaaggtcagttgaaagagttcctcgacgctaacctggccggc BBa_K906005_sequence 1 gtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt BBa_K906004_sequence 1 aagggcgagcttgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattga BBa_K906102_sequence 1 aagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagaaataattttgtttaactttaagaaggagatatacatacccatgggatctgataaaattattcatctgactgatgattcttttgatactgatgtacttaaggcagatggtgcaatcctggttgatttctgggcacactggtgcggtccgtgcaaaatgatcgctccgattctggatgaaatcgctgacgaatatcagggcaaactgaccgttgcaaaactgaacatcgatcacaacccgggcactgcgccgaaatatggcatccgtggtatcccgactctgctgctgttcaaaaacggtgaagtggcggcaaccaaagtgggtgcactgtctaaaggtcagttgaaagagttcctcgacgctaacctggccggcatgcaagtagaccccacaaaaggaatcggattagccaacacctctcttcagtttagtaatggccgcctgcatgccttgtgcgaatatgatctaccttacgtggtacggctgagtccggaagatggcgatatcagtaccgtgggtcgtatcgaaaacaatgtgtctaccaaaagtactaccgcacatcctaaaactgaccctgtgacgggagaaacctttagcttttcctacgggcccattcaaccctacgttacgtatagcagatatgattgcgacggcaaaaagagtggccccgatgttccgatattctcctttaaagaaccctctttcgtacatgactttgcaattactgagcactatgccgtgtttcctgatattcagattgtcatgaaaccagctgagattgttcggggtcggagaatgatcgggccagatttggaaaaagtcccccgtctggggctattaccgcgttatgccacctcagacagcgaaatgcgctggtttgacgtacccggttttaacatggtacacgtagtcaatgcgtgggaagaagagggaggcgaagtggtggtcattgttgccccgaatgtttcacccattgagaatgccattgatcgctttgatttgttacatgtgtccgtggagatggcgcggattgaactcaaatccggttccgtgagtcgcaccctactgtctgcggaaaacttagattttggagttattcatcggggttattctggtcgaaaaagcaggtacgcttacttgggcgtgggggatccaatgcccaaaattcgcggtgttgtgaaagtggacttcgaactggccggtcgaggggagtgtgttgtggctcggcgcgagtttggcgtcggctgtttcggaggggaacctttctttgtacccgcttccagcaagaagtcgggtggcgaagaagatgatggctatgttgtgagttacctccacgatgaaggcaagggtgaaagttcctttgtcgttatggacgcccgttcgccagaacttgaaatcttggctgaggtggtgttacccaggcgtgtcccctatgggtttcatgggttatttgtgacagaagcggaactcttgtcccaacaataaaagggcgagcttgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggtcatcatcaccatcaccattgagtttaaacggtctccagcttggctgttttggcggatgagagaagattttcagcctgatacagattaaatcagaacgcagaagcggtctgataaaacagaatttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt BBa_K906007_sequence 1 aagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagaaataattttgtttaactttaagaaggagatatacataccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z