BBa_K909008 1 BBa_K909008 tetR-DBD-UVR8 fusion protein 2012-09-22T11:00:00Z 2015-05-08T01:13:45Z tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10 cDNA of UVR8 was provided by Roman Ulm, University of Geneva. Released HQ 2013 Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch. false false _1174_ 0 14094 9 In stock false Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion false Gintautas Vainorius annotation2194628 1 tetR-DBD range2194628 1 1 390 annotation2194629 1 UVR8 range2194629 1 391 1674 BBa_K909008_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactaggatcccctcgtaaggttcttatcatctccgctggtgctagccactccgtcgctcttctctctggtgacattgtttgttcttggggtcgaggagaggatggacagttaggtcatggcgatgcagaggatcgaccttctccgactcagcttagcgctttagatggccaccaaattgtttccgttacctgtggtgctgatcacactgttgcttattcacaatcaggcatggaagtctacagttggggatggggtgattttgggagattaggccatggtaactcaagcgacttgtttactccgctaccaatcaaagcattgcacggtattcggatcaagcagattgcttgtggggatagtcattgtttggctgtcactatggaaggagaggtccagagttggggccgcaaccagaatggtcaacttggtctgggggacaccgaagattctctagtgcctcagaagattcaagcctttgagggaatacgaatcaaaatggttgctgctggtgcagaacacactgctgcagttacagaagatggtgacctctatggatggggctggggaagatacggaaatttgggattaggtgaccggactgaccgcttagttcctgaaagagttacctctactggtggtgagaaaatgtcaatggttgcttgtggatggcggcacacaatatcagtttcctactctggagcattgtatacttatggatggagcaaatatggacagctaggacatggagacttggaggatcaccttattcctcacaaactggaagcactgagcaacagttttatctcccagatttcgggaggttggagacatacaatggcattgacttcagatggaaaactatatggatggggttggaataagtttggacaagtaggagtcggcaataatttagatcagtgttctcctgtgcaagtgcgatttcccgatgatcagaaagtagttcaagtctcatgtggatggagacataccttggctgtcactgaaagaaataacgtgtttgcttggggtagaggtacaaatggacagctcggcattggagagtcggttgacaggaactttcccaagattatagaggcactcagcgtcgatggagcaagtggacaacatatagaatcttctaatatcgatccatcttcagggaaaagctgggtgtcgcctgcagagagatatgcagttgttcctgatgaaacgggcctaacggatggttcaagcaaaggtaatggaggtgatatcagtgttccacaaactgatgtcaagcgtgtacgaatttgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z