BBa_K909010
1
BBa_K909010
YcgZ promoter with multiple BluR (YcgE) operator sites
2012-09-22T11:00:00Z
2015-05-08T01:13:45Z
K238013 as template for PCR
Tschowri et al., 2009; Genes Dev 23: 522??? 534
Tschowri et al., 2012; Mol. Microbiol: 1-14; doi:10.1111/j.1365-2958.2012.08147.x
Escherichia coli senses blue light via the BLUF-EAL protein BluF (YcgF). The degenerate EAL domain of BluF does not have cyclic-di-GMP phosphodiesterase activity, but BluF directly antagonizes the MerR-like repressor BluR (YcgE), which leads to expression of the ycgZ-ymgABC operon
false
false
_1174_
0
14088
9
It's complicated
true
Based on the paper (Tschowri et al., 2012; Mol. Microbiol: 1-14) published in July 2012 in that they showed the binding site of BluR to the YcgZ promoter, more BluR binding sites were introduced to the YcgZ promoter to decrease leakiness of the promoter and therefore increase the repression by BluR.
false
Deborah Huber
annotation2194161
1
BluR operator site
range2194161
1
26
31
annotation2194166
1
BluR operator site
range2194166
1
69
73
annotation2194165
1
BluR operator site
range2194165
1
55
60
annotation2194163
1
-35
range2194163
1
28
33
annotation2194162
1
BluR operator site
range2194162
1
40
44
annotation2194164
1
-10
range2194164
1
52
57
BBa_K909010_sequence
1
attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgtacacatatttcgtacat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z