LOCUS       BBa_K909014             2699 bp    DNA     linear       22-May-2024
DEFINITION  Constitutive expression of pABA
ACCESSION   BBa_K909014
VERSION     BBa_K909014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..62
                     /label=Changed T to A 
     CDS             62..1951
                     /label=BBa_K137006
     misc_feature    1964..1967
                     /label=conserved
     RBS             1960..1971
                     /label=BBa_B0034
     CDS             1978..2562
                     /label=BBa_K137005
     stem_loop       2582..2625
                     /label=stem_loop
     misc_feature    2571..2650
                     /label=BBa_B0010
     terminator      2571..2650
                     /label=BBa_B0010
     stem_loop       2666..2685
                     /label=T7 TE
     misc_feature    2692..2692
                     /label=stop
     polyA_site      2686..2699
                     /label=polya
     misc_feature    2659..2699
                     /label=BBa_B0012
     terminator      2659..2699
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaggaa tttataataa aaaatacgga tatttggaaa attttcctaa aatattatcg
      121 gtcagatgaa gaaattgttt ttttacatag tagtcaggtg accgaaaatg agcattatag
      181 tattttagct cataaacctt ataaaaaggt ttcaaaatat aagggtcaag tgttttttaa
      241 tggtgaaaaa aagaagttca attttttaga tgcggtagat ttgctgaagg atgagagagt
      301 tgaacgacct aaaaattggc ctttttatcc agaattattg ggttttgtaa gttatgagca
      361 agatccggct tgttttgcag cttatgatga agttttactt tttgaccata gaacaaaact
      421 gttacgtgtc gtgcaatttg aacaaactga tggccaatat tggctgactg aatcggaaga
      481 aattgaagtg gattcagaga ttgagttcga tgttcagaat ggcatagggg ctgtttttat
      541 tgaccaaacg aggcaagaat atattgcttc aataaaaaaa atacaagatt atatgaaggc
      601 tggcgatatt tatgttgcca atttgacgca acaatttgaa atttggtccg atcaaaaacc
      661 tattgatgtt tttaagaaaa caaggaaaca aattcctgct cctttctcat catttttaca
      721 atatcctgaa tggaaaatga cgcaaatttc aagttctgtt gaacgctttg tttctattca
      781 taatggagca ttaatttcaa aacccattaa aggaacaatt gccagaggag aaaatgttgt
      841 agctgataga ttacaaaaga aaatactttc cgacagtagt aaagaacggt ctgagttact
      901 gatggtgacg gatttattgc gtaatgatat tgcccgaatt agtcaacctt tttctttgtc
      961 tgtccctaaa tttgcagaaa ttgaaacttt ttctcatgtt catcaactgg tgacttcaat
     1021 aaaaagtaga attaaagaag atttgacttt ttcagaattt atgacggctt tatttccggg
     1081 aggttcaatc acaggtacac ctaagaagcg ggcaatggag attattaagg aagttgaaaa
     1141 acaacctaga ggaatttata cagggatgca gggttggtta agtcgagaaa tggatttgga
     1201 tatgaacatc gtgattcgga cattagttca tgacggtgag cattatcagc ttggtgtagg
     1261 cggtggaatt acgttcgaga gcgaagcaga agcagagttt tcagagattt tactaaaagc
     1321 aaaacccttt ttagatatat taggcctgaa agatgttcca agtattttat ttacaacagg
     1381 ccttgttaaa aatggtgaat tgttaaattt agagggtcat gtcaatcgct tgaaaaaaca
     1441 ataccatcat gcagatttgg aagaaaaatt gagaatattt gcccaaaagg ttactgacgg
     1501 ggttttacgt atcagtactg acggagattc tttgacccct gggattcgtc agttgaccca
     1561 ttcaaatgag gcctatcgag ttaaactgtc gtcaattaat gacaaaccta gtcttttgtc
     1621 taactttaaa ctttcaggcc cagattttca aaaggtcttt cgtcaggaag tcttagaagc
     1681 gaaaaaagaa gggtttcaag atattctatt tcatacagat ggtttgatca gtgaattatc
     1741 aatcggaaat tttgtggcaa aaaaggggaa tcagtacgaa acgccagcca aatatgctct
     1801 aaaaggaact tttttagatt tatttgcgaa aaatcatacg ctaatatata aagatattgc
     1861 gatttctgat ttgaaaactt acgatcgttt ttatatgaca aatgctgtca gaggattagt
     1921 tgaaataaaa attgatggaa tttcctaata atactagaga aagaggagaa atactagatg
     1981 aaattgctat taattgataa ttatgattct tttacttatt tgcttgcaca gtattttgag
     2041 gaattagatt gtagtgtaac agtggttaat gaccaagata aaatgagtca gaaaattaga
     2101 atttctccgg attttatttg tgaaaattat gatgcgatta tcatttctcc aggtccaaaa
     2161 acaccaaaag aagccgtttt tagtagggat gtagttcaac tatacgctgg aaaaattcct
     2221 atgttaggaa tttgcttggg tcagcaagtc attgctgaat gttttggagg aaatgttgtt
     2281 cttggtgaga ggccaatgca tggaaaaatt tctgtcattc gtcataatta tcaaggaatt
     2341 tttaaaggac ttccacaaaa tttgaaagtt gcacgttatc attcgttgat tgtcgataaa
     2401 ctacctgatg attttgaaat tgatgcgcag agtgaggatg gtgttattca ggccattcat
     2461 cagccaaaat taaaattgtg ggctttacaa tttcacccag aaagtttagt tactgaatat
     2521 ggtcatgaaa tgttaaataa ttttttgaaa gtggtgtaat aatactagag ccaggcatca
     2581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2641 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//