BBa_K911002
1
BBa_K911002
Magnesium sensitive riboswitch (8 codon substitution)
2012-09-11T11:00:00Z
2015-05-08T01:13:45Z
Bacillus Genome
Riboswitch that acts as a regulatory element, truncating transcripts when magnesium is not bound to the RNA. Four Mg2+ binding sites exist, giving this part significantly switch-like behavior. Binding of these sites by Mg2+ results in compaction of the regulatory region of the riboswitch, which in its unbound state acts as an anti-terminator. Loss of this anti-terminator activity results in the activity of a downstream terminator (included in this sequence) which terminates transcription of the gene. May also be affected by other divalent ions, such as manganese.
false
false
_1176_
0
12694
9
It's complicated
false
Original paper mentioned 8 codon substitution of the downstream LacZ reporter gene with the first 8 codons of the MgtE gene (which is the native gene which this riboswitch controls). Our construct used LacI as the downstream regulated sequence, which requires its N-terminal domain to bind to DNA. Sequences both with and without this substitution have been submitted. This contains the substitution. Please note that to use this version effectively, an assembly method that does not leave scars (such as Gibson assembly) will be required.
false
Oliver Meacock
annotation2182731
1
Terminator
range2182731
1
284
299
annotation2182729
1
8 codon substitution.
range2182729
1
487
510
annotation2182730
1
M-box
range2182730
1
95
250
BBa_K911002_sequence
1
tgttccgtaattgtgatgtaagcgcatttattacttttttgagttgacatagcttgtttttttctgtaatctcagtgtgtcaattcaattaggaacttcgttaggtgaggctcctgtatggagatacgctgctgcccaaaaatgtccaaagacgccaatgggtcaacagaaatcatcgacataaggtgatttttaatgcagctggatgcttgtcctatgccatacagtgctaaagctctacgattgaaggcgcccgcacgctttttttgccgtgcttctttcaccttcaatcccgaaggctttttttatgcctttaaaacgaaaccaatcaaaggaggtgcggagtatggattctccccattcgagtcaatctgaagaagtacccatatactatgacagcaagacaattgattacacgatgaggagacgaactgccgcaccttctggcgaggccggcacgtctccgtagaggaggtacgagtcccgatggttcaaaacatgacctatgac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z