BBa_K911003
1
BBa_K911003
Fluoride Sensitive Riboswitch
2012-09-11T11:00:00Z
2015-05-08T01:13:45Z
Bacillus Cereus Genome
Released HQ 2013
Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.
false
false
_1176_
0
12694
9
In stock
true
Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.
false
Oliver Meacock
annotation2182733
1
Fluoride riboswitch
range2182733
1
61
139
annotation2182732
1
Lysine promoter
range2182732
1
1
43
BBa_K911003_sequence
1
aaaaataatgttgtccttttaaataagatctgataaaatgtgaactaaatgtaataattataggcgatggagttcgccataaacgctgcttagctaatgactcctaccagtatcactactggtaggagtctatttttttgagcaagctatttaaagagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z