LOCUS BBa_K911004 8715 bp DNA linear 25-Nov-2024 DEFINITION Synthesised Ratiometric Luciferase construct in non-standard plasmid ACCESSION BBa_K911004 VERSION BBa_K911004.1 FEATURES Location/Qualifiers promoter 1..97 /label=HyperSpank RBS 98..126 /label=Bacillus consensus RBS + Spacers CDS 127..834 /label=mOrange no stop misc_feature 835..864 /label=GS Linker CDS 865..1932 /label=LuxA (fused to mOrange) misc_feature 1933..2062 /label=B0015 terminator promoter 2063..2159 /label=pVEG RBS 2160..2188 /label=Bacillus consensus RBS + Spacers CDS 2189..3622 /label=LuxC RBS 3623..3651 /label=Bacillus consensus RBS + Spacers CDS 3652..4569 /label=LuxD RBS 4570..4598 /label=Bacillus consensus RBS +Spacers CDS 4599..5666 /label=LuxA (normal) RBS 5667..5695 /label=Bacillus consensus RBS + Spacers CDS 5696..6670 /label=LuxB RBS 6671..6699 /label=Bacillus consensus RBS + Spacers CDS 6700..7836 /label=LuxE RBS 7837..7865 /label=Bacillus consensus RBS + Spacers CDS 7866..8585 /label=LuxG misc_feature 8586..8715 /label=B0015 terminator ORIGIN 1 caacctaatt gtgagcgctc acaatttttt gcaaaaagtt gttgacttta tctacaaggt 61 gtggcataat gtgtgtaatt gtgagcggct cacaatttac tagaggggaa aaggaggtga 121 actactatgg tgtctaaagg cgaagaaaac aacatggcga taatcaaaga gtttatgcgc 181 tttaaagtca ggatggaagg ctccgtcaat ggtcacgaat ttgaaattga gggagaaggt 241 gaaggacggc cgtacgaggg atttcaaaca gctaaactta aagtaacaaa aggcggaccg 301 cttccgtttg catgggacat tttgagtcct cagtttacgt atggttctaa agcgtatgtc 361 aaacatcccg cggatattcc ggattacttt aagctctcgt ttcctgaagg gttcaagtgg 421 gagagagtaa tgaatttcga ggatggtgga gtagttaccg tcacgcaaga ctcatcatta 481 caagacggag agttcattta caaagttaaa ctgcgaggta caaacttccc gtccgatggg 541 cctgtgatgc agaagaaaac tatgggatgg gaagcatcta gcgaaagaat gtatccagag 601 gatggcgctc tgaaaggcga gatcaaaatg cggctaaaac tgaaagacgg cggacattat 661 acgtcagaag ttaaaaccac ttacaaagcc aagaagcctg tgcaattacc aggggcatat 721 atcgtgggga ttaagcttga tattacaagc cataatgaag attatacgat cgttgaacag 781 tatgaacgtg ccgaaggccg ccacagcaca ggcggaatgg atgaattgta taagggcggc 841 ggcggctcag gcggcggcgg ctcaatgaag tttggtaact ttctgttgac gtatcagccg 901 ccagaactca gtcaaacaga ggttatgaaa cgcctcgtca atttaggaaa agcttccgaa 961 gggtgcggct ttgacacagt atggttgctg gaacaccatt ttaccgaatt tggcctgctg 1021 ggcaatccat atgtcgcggc agctcatctt cttggagcga cggaaaaact caacgtagga 1081 actgccgcca ttgtattacc taccgctcat ccggttagac aggcagagga tgttaatctt 1141 ttagaccaga tgtctaaagg taggttcaga tttggcattt gccgcggttt atacgataaa 1201 gattttcggg tgttcggaac ggatatggac aatagcagag cgttaatgga ttgctggtac 1261 gatttgatga aagagggatt taacgaaggc tatattgccg cagacaatga acatatccaa 1321 tttcctaaaa tccagttaaa tccgtcagca tatacgcaag gcggtgcacc tgtgtatgtc 1381 gtcgcggagt cggcttctac tacagaatgg gctgccgaac gaggtttgcc gatgattctt 1441 tcctggatca ttaacactca cgaaaagaaa gcacaattag atctgtataa tgaggttgct 1501 acagagcatg gatatgacgt gactaagatt gaccactgtc tttcttacat tacaagcgtc 1561 gatcacgatt caaacaaagc caaagatatt tgtcgtaact ttcttggcca ttggtacgac 1621 tcatatgtta atgcgacaaa aatcttcgac gattcggacc aaaccaaggg ctatgatttc 1681 aacaaggggc agtggcggga ttttgtgctg aaggggcata aagatacaaa tagacgtatt 1741 gattattctt acgagataaa tccggtgggg acacctgaag aatgtattgc gatcatacaa 1801 caggatatcg acgccacggg cataaacaac atttgctgtg gatttgaggc gaatggaagc 1861 gaagaagaaa tcatcgcatc aatgaaattg ttccagagcg atgtaatgcc ctaccttaaa 1921 gaaaaacaat aagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1981 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 2041 ggtgggcctt tctgcgttta taaattttgt caaaataatt ttattgacaa cgtcttatta 2101 acgttgatat aatttaaatt ttatttgaca aaaatgggct cgtgttgtac aataaatgtt 2161 actagagggg aaaaggaggt gaactactat ggagaagcat ctaccactca ttatcaatgg 2221 ccaaattgta tttaccgaag aaaaccgttt tgagatatct tttgaagaaa agaaagtcaa 2281 gattgactca ttcaatcatg tccatctgac acaaatggtt actcatgatt atctcaacga 2341 cataaacatt aacaatatca tcaacttctt atacacgacc ggccagcggt ggaaaagcga 2401 agaatatagc cgaaggcggg cttacattag atcgcttgtc acatacctgg gatatagtcc 2461 tcaaatggct aaactcgaag ccaattggat cgcgatgata ctttgcagta agtctgcact 2521 gtatgatatc atcgacaccg aacttggatc gacacacatt caggatgagt ggttgccgca 2581 aggggaatgc tacgtccgcg cgttcccaaa aggtcgtaca atgcacttac tagccgggaa 2641 tgtcccgctg agcggcgtga catctatttt gcgaggtatc ttaacgcgga atcagtgcat 2701 tgtgaggatg tccgcaagcg atccttttac ggcacatgcg ctcgccatgt cgtttatcga 2761 tgtggacccg aaccatccaa ttagtcgctc tatttcggta ctttattggc ctcacgctag 2821 tgacacaacc ttggcagaag aactgctcaa tcacatggac gctgtcgttg cctggggtgg 2881 ccgtgacgcg atcgattggg cggttaaaca ttcaccgtcc catatagatg ttctgaaatt 2941 tggacccaag aagtccttca cggtccttga tcacccggca gatttagaag aggctgcgtc 3001 cggtgttgca catgacatat gtttttacga tcagaacgca tgctttagca cacagaacat 3061 ttacttttct ggcgataact atgaagaatt taagagcaaa ttggttgaaa aactgaacct 3121 ctatcaagag gtccttccta aatctaagca gaattttgat gatgaggcgc tcttttcaat 3181 gacgcgattg gagtgtcagt ttagcggact caaagtaata tcagaaccgg aaaacaattg 3241 gatgattata gattcagaac cgggcgtaga gtacaatcat ccgttgagcc gttgtgtcta 3301 tgtacacaaa atcaataaag ttgacgacgt tgtgcagtat atcgaaaaac atcaaacgca 3361 aacgatttct ttctatccct gggaaagttc gaaaaagtac agagatgcgt tcgccgcgaa 3421 aggcgttgag aggattgttg agtcgggaat gaataacatt tttcgcgttg gtggtgcaca 3481 cgatgctatg cgccctctcc aacggttagt ccgcttcgta tcacacgaaa gaccttatca 3541 ttttacaacc aaagatgtta gcgtggagat cgaacaaacg cggtttctgg aagaagataa 3601 gtttttagtg tttgtgcctt aatactagag gggaaaagga ggtgaactac tatgtcaaac 3661 caatgtaaaa caatagctca tgtgctccca gtgaataatg gacaagaaat tcacgtctgg 3721 gaaactccgc caaaagaaaa tgctccgtct aagaatagca cgattctgat tgcctctgga 3781 tttgccagac gcatggatca ttttgcaggc cttgcagaat acttatctga gaacggtttc 3841 cacgttttca ggtacgattc gctacatcat gtaggactta gtagcggctc aattgatgaa 3901 tttacaatga cgactggtaa gaactccctg tgcaccgtgt atcattggtt gcagaccaaa 3961 gggacgcaga atataggcct catcgccgct agcctgagtg cgcgggtagc ctatgaagta 4021 atcagcgatt tagagctcag ctttctcatt acggcagtcg gggttgttaa cttgcgcgac 4081 acacttgaga aagcgctggg gtttgactat ctctcattgc cgattaatga actgcctaat 4141 gatctggatt tcgaaggcca taagttgggt tccgaagtgt tcgttcggga ctgttttgag 4201 caccattggg atacattaga ttcaacacta gacaaagtcg caaatactag cgtgcctctt 4261 atcgcattta cagccaacaa tgacgattgg gtcaagcaag aagaagtgta cgacatgtta 4321 gctcatatca gatctggaca ctgcaaactt tattcacttc tgggatcaag tcatgattta 4381 ggcgaaaact tagtcgtatt gagaaacttc tatcaatccg ttacaaaagc ggcgattgcg 4441 atggatggcg gatctctgga gattgatgtc gactttattg aacccgattt tgagcagtta 4501 accatcgcaa cggttaacga acgtcgactt aaagcggaga tcgaatcgcg tgctccggaa 4561 atggcataat actagagggg aaaaggaggt gaactactat gaaattcgga aactttttac 4621 tgacctatca gccaccagaa ttatcccaga ctgaagttat gaaaagatta gtaaatctgg 4681 gcaaagcttc agaaggctgc ggatttgata cagtgtggct tctcgaacat cattttacgg 4741 aatttgggct actcggtaat ccgtacgtcg cagccgcaca cttactgggc gcaacggaaa 4801 agctgaatgt cggaacagca gcgattgtac ttccgaccgc ccatcctgtt cgccaggcag 4861 aagatgttaa cttactggat caaatgagta aaggtcggtt tcgtttcggt atctgccgcg 4921 gactatacga caaagatttt agggtgtttg gcactgatat ggataacagc agggcgttga 4981 tggattgttg gtatgatctg atgaaagaag gcttcaatga ggggtatatc gccgctgaca 5041 acgaacatat tcagttcccg aagattcaac ttaatccgtc ggcatataca cagggtggag 5101 cgcccgtgta tgttgttgct gagtctgcga gcacaactga atgggcagcg gaacggggac 5161 tgcctatgat acttagctgg atcataaaca cacatgagaa aaaggcacaa cttgacttgt 5221 acaatgaagt ggcaacggaa cacgggtacg atgtgaccaa aatcgatcac tgtctttcat 5281 acattacgtc agtagatcat gattccaata aagccaagga catttgtcga aactttttag 5341 gccactggta cgacagctat gtcaatgcga ctaagatttt tgacgatagc gatcagacaa 5401 aaggctatga cttcaataaa ggccaatgga gagactttgt cctgaagggc cataaagaca 5461 cgaacagacg catcgactat tcttatgaga ttaatcctgt cggaacacca gaagagtgta 5521 tcgctatcat tcaacaagat atcgatgcga caggaattaa caacatctgc tgcgggttcg 5581 aggccaatgg ctctgaagaa gagattattg cttcaatgaa actttttcaa tctgatgtca 5641 tgccgtacct aaaagagaaa cagtaatact agaggggaaa aggaggtgaa ctactatgaa 5701 attcggatta ttctttctta actttatgaa cagcaaacgc agcagtgatc aagttatcga 5761 agaaatgcta gatacagcgc attatgtcga tcagttgaaa tttgataccc ttgcggtata 5821 tgagaatcac tttagcaata acggcgtggt tggggcacct ttaacggttg ctggatttct 5881 tctcggtatg accaagaatg caaaagtcgc atctctgaat catgtcataa caacacatca 5941 tccagtgagg gtggcggaag aggcatgcct tctggaccaa atgtcagagg ggcgctttgt 6001 atttggattc agtgactgcg aaaaatccgc tgacatgcgt ttctttaaca ggcctactga 6061 ctcccaattt cagcttttta gtgaatgcca caagatcatt aacgatgcct ttacaactgg 6121 atattgtcac ccgaataatg acttctattc ttttcccaaa atctcagtga acccgcatgc 6181 ctacacggaa ggtggtccgg cgcaatttgt caacgcaacg agcaaagagg ttgtggagtg 6241 ggcagccaaa cttgggttgc ctttggtctt taagtgggat gattcgaatg cgcagcggaa 6301 agaatatgcc ggcttgtatc atgaagtggc gcaagcacac ggcgtagatg tatcgcaagt 6361 gagacataag ttaacactgc tggttaatca aaacgtcgac ggcgaagctg cgcgtgctga 6421 agccagagtc tacttagaag agttcgtacg ggagtcttac ccgaataccg attttgaaca 6481 gaaaatggtt gaactactgt cagaaaatgc cattggcact tatgaagaat ctacacaagc 6541 agcaagagta gccattgaat gttgtggagc tgctgacctc ttaatgtcat tcgagtcaat 6601 ggaagataaa gctcagcagc gagcggttat tgatgtggtc aacgctaata tcgtgaagta 6661 ccatagctaa tactagaggg gaaaaggagg tgaactacta tggaagtcct gagcgcggta 6721 aaacaggaaa acattgcagc atcaacggaa attgacgatt tgatctttat gggaacacct 6781 caacaatggt ccctacagga acagaaacaa ctcacatcac gccttgttaa aggggcatat 6841 caatatcatt atcacaataa tgatgattat cggcaattct gccaacgtca gggagtgggc 6901 gaggtggttg aagatctgaa cgaaatcccg gtttttccca caagcatctt taaactcaaa 6961 acattgttaa ctttagatga tgatgaagtg gaaaacagat tcacaagtag tggtacctct 7021 ggtatcaaaa gcattgtagc gagagatcgc ttatccattg aaagattact ggggtcagtc 7081 aactttggca tgaactacgt cggggactgg ttcgatcatc aaatggaatt ggtgaattta 7141 ggccctgatc ggtttaacgc aaataacatc tggtttaagt atgtaatgtc tcttgttgag 7201 ctgctgtatc ccaccgcctt tacggtgaca gaggacgaaa ttgactttga agcgacactt 7261 gccaatatga atagaatcaa acagtcggga aaaaccatat gtcttattgg tccgccatac 7321 ttcatctacc tgctttgttg cttcatgcgt gaacaagggc agacgttcaa tggcggacgg 7381 gacttataca taatcacggg cggtggatgg aaaaagcatc aggatcagag cttggaccgt 7441 gacgagttta atcagttact atgcgaaacc tttacattag aatcagccga gcaaattagg 7501 gatactttta accaggtcga actaaatact tgtttctttg aggacacaga acacaagaaa 7561 cgcgtaccgc cttgggtctt tgctagggcc ttggatccaa agacgttgaa accactgccg 7621 catggacaac cgggacttat gtcctatatg gacgctagcg cggtgtctta tccgtgtttt 7681 cttgttacgg atgatattgg tattgtacgc gaagaagagg gcgatcgtcc tggcactaca 7741 gttgagattg tacgaagagt caaaacgcga ggcatgaaag ggtgcgcgct gtcgatgtct 7801 caagctttta cagctaagaa tgagggcgga aactaatact agaggggaaa aggaggtgaa 7861 ctactatgaa agaggccaca gatatgcttt gctccatcga gaagatagaa cctttgacaa 7921 atctgatctt ccgtgtctta ctgaaaccgg atcagccttt tgaatttaga gcggggcaat 7981 acatcaatgt aagcctttct ttcggatcac tcccattttc tatagcgagc tgcccgtcaa 8041 atggggcatt cttggagctc cacattggtg gatcagacat ttccaagaaa aacacgcttg 8101 tgatggaaga attgaccaat agctggattt gcggaaacat ggttgaagtt agcgaggcga 8161 gaggcgacgc atggttacga gatgaaagtg taaaacctct actgctggtt gctggcggaa 8221 ctggcatgag ttacacttta tcgatcttga aaaactctct cgaacaaggc tttattcaac 8281 ccatttatgt ttattggggt gctaaagaca tggataatct gtatgtgcat gatgaacttg 8341 tggatattgc cttagaaaac aagaatgttt cctatgtccc agtcacagag atctctacgt 8401 gtccgcagta cgctaaacag ggaaaagtct tagaatgtgt aatgtcagat tttcgcaacc 8461 tttcggagtt tgacatttat ctgtgtggcc cgtgtaagat ggtggaagtc gcacgggatt 8521 ggttctgcga caaaaggggt gccgaaccgg aacaaatcta cgcggatgca tttgcttatc 8581 tttaagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 8641 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 8701 ctttctgcgt ttata //