BBa_K911006 1 BBa_K911006 Non-standard backbone for luciferase construct 2012-09-22T11:00:00Z 2015-05-08T01:13:45Z synthesis by DNA 2.0 This is the sequence of the non-standard backbone that our luciferase construct, BBa_k911004, was synthesised in. It could not be assembled in the high copy number standard psb1c3 backbone due to toxicity issues. Attempts to assemble the 9kb insert into psb3c5, a low copy number biobrick plasmid have so far been unsuccessful. The sequence of the low copy number custom backbone is provided here as a reference. false false _1176_ 0 12683 9 Not in stock false We did not design this sequence - however, it was chosen to assemble in as it is maintained at a low copy number, avoiding fatal toxicity. false Thomas Whittaker annotation2194222 1 rpn txn terminator range2194222 1 405 518 annotation2194224 1 Element #6 range2194224 1 519 580 annotation2194219 1 Biobrick Suffix range2194219 1 1 21 annotation2194225 1 p15a ori range2194225 1 581 1407 annotation2194221 1 bla txn terminator range2194221 1 98 398 annotation2194223 1 Element #5 range2194223 1 399 404 annotation2194226 1 KanR range2194226 1 1412 2221 annotation2194229 1 rrnB1 B2 txn terminator range2194229 1 2400 2574 annotation2194231 1 Biobrick Prefix range2194231 1 2650 2671 annotation2194228 1 Pamp range2194228 1 2222 2399 annotation2194220 1 Element #4.2 range2194220 1 26 97 annotation2194230 1 Element #3.1 range2194230 1 2575 2645 BBa_K911006_sequence 1 tactagtagcggccgctgcagaaaatgaagggaagttcctatactttctagagaataggaacttctatagggagtcgaataagggcgacacaaaaggtattctaaatgcataataaatactgataacatcttatagtttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctttattattttcgagatttattttcttaattctctttaacaaactagaaatattgtatatacaaaaaatcataaataatagatgaatagtttaattataggtgttcatcaatcgaaaaagcaacgtatcttatttaaagtgcgttgcttttttctcatttataaggttaaataattctcatatatcaagcaaagtgacaggcgcccttaaatattctgacaaatgctctttccctaaactccccccataaaaaaacccgccgaagcgggtttttacgttatttgcggattaacgattactcgttatcagaaccgcccaggatgcctggcagttccctactctcgccgctgcgctcggtcgttcggctgcgggacctcagcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaagcttttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgaggcgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgagtgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaacgctgtttttccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagtggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctcgacgtttcccgttgaatatggctcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtcagtgttacaaccaattaaccaattctgaacattatcgcgagcccatttatacctgaatatggctcataacaccccttgtttgcctggcggcagtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggactccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgcccgggctaattagggggtgtcgcccttattcgactctatagggaagttcctattctctagaaagtataggaacttctgaagggggggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z