LOCUS       BBa_K911009             1819 bp    DNA     linear       25-Nov-2024
DEFINITION  Fluorescent ratiometric construct for standardizing promoter output
ACCESSION   BBa_K911009
VERSION     BBa_K911009.1  
FEATURES             Location/Qualifiers
     RBS             2..8
                     /label=rbs
     RBS             1..11
                     /label=BBa_K143020
     CDS             18..740
                     /label=BBa_E0020
     stem_loop       760..803
                     /label=stem_loop
     misc_feature    749..828
                     /label=BBa_B0010
     terminator      749..828
                     /label=BBa_B0010
     stem_loop       844..863
                     /label=T7 TE
     misc_feature    870..870
                     /label=stop
     polyA_site      864..877
                     /label=polya
     misc_feature    837..877
                     /label=BBa_B0012
     terminator      837..877
                     /label=BBa_B0012
     terminator      749..877
                     /label=BBa_B0015
     promoter        948..953
                     /label=Sigma A-35
     promoter        971..976
                     /label=Sigma A -10
     promoter        886..982
                     /label=BBa_K143012
     RBS             991..1002
                     /label=rbs
     RBS             991..1002
                     /label=BBa_K143021
     misc_feature    886..1002
                     /label=BBa_K143053
     CDS             1009..1731
                     /label=BBa_E0030
     stem_loop       1751..1794
                     /label=stem_loop
     misc_feature    1740..1819
                     /label=BBa_B0010
     terminator      1740..1819
                     /label=BBa_B0010
ORIGIN
        1 taaaggagga atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tcctggtcga gctggacggc gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg
      121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      301 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg
      481 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
      541 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      601 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
      661 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      721 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      781 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      841 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaattt tgtcaaaata
      901 attttattga caacgtctta ttaacgttga tataatttaa attttatttg acaaaaatgg
      961 gctcgtgttg tacaataaat gttactagag aaaggtggtg aatactagat ggtgagcaag
     1021 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1081 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1141 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1201 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
     1261 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1321 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1381 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1441 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1501 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc
     1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1801 tttgtcggtg aacgctctc
//