LOCUS       BBa_K912029             3044 bp    DNA     linear       17-May-2024
DEFINITION  Pgad/lysis
ACCESSION   BBa_K912029
VERSION     BBa_K912029.1  
FEATURES             Location/Qualifiers
     misc_feature    1249..1251
                     /label=gadC Start
     promoter        141..158
                     /label=PgadR
     CDS             275..1105
                     /label=gadR
     promoter        1163..1181
                     /label=Pgad
     RBS             1237..1245
                     /label=RBS_gadC
     RBS             264..269
                     /label=RBS_gadR
     promoter        1..1251
                     /label=BBa_K559011
     CDS             1276..1932
                     /label=T4 holin
     CDS             1260..1935
                     /label=BBa_K112805
     CDS             1960..2454
                     /label=T4 Endolysin
     CDS             1944..2457
                     /label=BBa_K112806
     stem_loop       2477..2520
                     /label=stem_loop
     misc_feature    2466..2545
                     /label=BBa_B0010
     terminator      2466..2545
                     /label=BBa_B0010
     stem_loop       2561..2580
                     /label=T7 TE
     misc_feature    2587..2587
                     /label=stop
     polyA_site      2581..2594
                     /label=polya
     misc_feature    2554..2594
                     /label=BBa_B0012
     terminator      2554..2594
                     /label=BBa_B0012
     promoter        2603..2637
                     /label=BBa_J23116
     CDS             2660..2953
                     /label=T4 antiholin
     CDS             2646..2956
                     /label=BBa_K112807
     stem_loop       2976..3019
                     /label=stem_loop
     misc_feature    2965..3044
                     /label=BBa_B0010
     terminator      2965..3044
                     /label=BBa_B0010
     misc_feature    1260..3044
                     /label=BBa_K112808
ORIGIN
        1 tgagcgttgt ataagctttt atgtctttct atatcaactt ttaatagaaa tataaagtaa
       61 tataaatgtt tttataataa attatgtgag atatattttt ttgtccgtac tggtatagat
      121 ttgacgatta agtcttaaat aagttataat ctcaattgcg taatttctta aatacagaaa
      181 taacaactac attggtagac tgattaaaaa gtgtacttga tgaactgtta taaaccttaa
      241 aaaaataaaa ataatagttt gggggatgtt aaagatgtat aaaaaatatg gagattgttt
      301 taaaaagttg cgaaaccaaa agaatttagg gttatcatac tttagtaaac ttggaataga
      361 ccgttcaaat atatcaagat ttgaacatgg aaaatgtatg atgagttttg agcgtataga
      421 tttgatgtta gaagaaatgc aagttccgtt atctgagtac gaattgattg taaataattt
      481 tatgccgaat ttccaagaat tttttatatt agaattggaa aaagctgaat ttagccaaaa
      541 tcgagataaa ataaaagagt tgtattctga ggtcaaagaa acggggaatc atttactgac
      601 ggttaccgtg aaaacgaagc ttgggaatat aagtcagaca gaagttaaag aaattgaagc
      661 ttatctttgc aatattgaag agtggggata ttttgaactt actttatttt attttgtatc
      721 tgattatctc aatgtcaatc aattagaatt gctgcttttt aattttgata aaagatgtga
      781 aaattactgt agagtcttaa aatatagaag gagactattg caaatagcct ataaaagtgt
      841 tgcgatatac gcggctaaag gagaaagaaa aaaagccgaa aatattttag aaatgactaa
      901 aaaatatcga actgtgggag tcgatttata ttcagaagta ttaagacatc ttgctagagc
      961 tatcattatt tttaattttg aaaatgcaga gattggggaa gaaaaaataa attatgctct
     1021 tgagattttg gaagaatttg gaggaaagaa gataaaagaa ttttatcaga ataaaatgga
     1081 aaagtatttg aaaaggtcaa tttagtctct tttgagctgt tgctttaaag caacagctca
     1141 aaagagattt tctttattcc agagcatata ctagagggtg aagataggtt gtctgaagca
     1201 ttataacttg tcttttaaaa aattcaatca taaatataag gaggtatgat gtactagagc
     1261 ttaaaaggag ggtctatggc agcacctaga atatcatttt cgccctctga tattctattt
     1321 ggtgttctcg atcgcttgtt caaagataac gctaccggga aggttcttgc ttcccgggta
     1381 gctgtcgtaa ttcttttgtt tataatggcg attgtttggt ataggggaga tagtttcttt
     1441 gagtactata agcaatcaaa gtatgaaaca tacagtgaaa ttattgaaaa ggaaagaact
     1501 gcacgctttg aatctgtcgc cctggaacaa ctccagatag ttcatatatc atctgaggca
     1561 gactttagtg cggtgtattc tttccgccct aaaaacttaa actattttgt tgatattata
     1621 gcatacgaag gaaaattacc ttcaacaata agtgaaaaat cacttggagg atatcctgtt
     1681 gataaaacta tggatgaata tacagttcat ttaaatggac gtcattatta ttccaactca
     1741 aaatttgctt ttttaccaac taaaaagcct actcccgaaa taaactacat gtacagttgt
     1801 ccatatttta atttggataa tatctatgct ggaacgataa ccatgtactg gtatagaaat
     1861 gatcatataa gtaatgaccg ccttgaatca atatgtgctc aggcggccag aatattagga
     1921 agggctaaat aattatacta gagatactta ggaggtatta tgaatatatt tgaaatgtta
     1981 cgtatagatg aaggtcttag acttaaaatc tataaagaca cagaaggcta ttacactatt
     2041 ggcatcggtc atttgcttac aaaaagtcca tcacttaatg ctgctaaatc tgaattagat
     2101 aaagctattg ggcgtaattg caatggtgta attacaaaag atgaggctga aaaactcttt
     2161 aatcaggatg ttgatgctgc tgttcgcgga atcctgagaa atgctaaatt aaaaccggtt
     2221 tatgattctc ttgatgcggt tcgtcgctgt gcattgatta atatggtttt ccaaatggga
     2281 gaaaccggtg tggcaggatt tactaactct ttacgtatgc ttcaacaaaa acgctgggat
     2341 gaagcagcag ttaacttagc taaaagtaga tggtataatc aaacacctaa tcgcgcaaaa
     2401 cgagtcatta caacgtttag aactggcact tgggacgcgt ataaaaatct ataaagctac
     2461 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2521 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2581 tttctgcgtt tatatactag agttgacagc tagctcagtc ctagggacta tgctagctac
     2641 tagaggatag gaggccttta tggccttaaa agcaacagca ctttttgcca tgctaggatt
     2701 gtcatttgtt ttatctccat cgattgaagc gaatgtcgat cctcattttg ataaatttat
     2761 ggaatctggt attaggcacg tttatatgct ttttgaaaat aaaagcgtag aatcgtctga
     2821 acaattctat agttttatga gaacgaccta taaaaatgac ccgtgctctt ctgattttga
     2881 atgtatagag cgaggcgcgg agatggcaca atcatacgct agaattatga acattaaatt
     2941 ggagactgaa tgaaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     3001 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctc
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