BBa_K559011 1 Pgad Pgad - Intracellular Chloride-Sensing Cassette 2011-10-27T11:00:00Z 2015-05-08T01:12:41Z The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG. Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated. false false _727_ 0 6123 9 It's complicated true Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli. false Jacky, Fong Chuen, Loo annotation2160941 1 RBS_gadR range2160941 1 264 269 annotation2160939 1 Pgad range2160939 1 1163 1181 annotation2160937 1 PgadR range2160937 1 141 158 annotation2160936 1 gadC Start range2160936 1 1249 1251 annotation2160940 1 RBS_gadC range2160940 1 1237 1245 annotation2160938 1 gadR range2160938 1 275 1105 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_K112807 1 BBa_K112807 [T4 antiholin] 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts Antiholin binds holin and prevents holin multimers from forming pores on the inner membrane of bacteria. false true _224_ 0 3025 171 It's complicated false Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device. false Jin Huh annotation1999019 1 T4 antiholin range1999019 1 15 308 BBa_K112805 1 BBa_K112805 [T4 holin] 2008-10-30T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=160221&to=160877&view=gbwithparts Holins from T4 bacteriophage assemble together to form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. false true _224_ 0 3025 171 It's complicated false Making composite part of rbs.holin is problematic unless antiholin is expressed in bacteria together. Antiholin binds holin and inhibits the formation of holin. false Jin Huh annotation1999005 1 T4 holin range1999005 1 17 673 BBa_K112806 1 BBa_K112806 [T4 endolysin] 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts Released HQ 2013 The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer. false true _224_ 0 3025 171 In stock false High expression of lysozyme only is toxic to bacteria. false Jin Huh annotation1999018 1 T4 Endolysin range1999018 1 17 511 BBa_K912029 1 BBa_K912029 Pgad/lysis 2012-10-02T11:00:00Z 2015-05-08T01:13:45Z Pgad: PCR from vector from CUHK 2011 team Lysis: registry This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high intracellular chloride concentrations. false false _1177_ 0 12923 9 Not in stock false None besides primer design false Grant Nicholas component2210193 1 BBa_K559011 component2210209 1 BBa_K112808 annotation2210209 1 BBa_K112808 range2210209 1 1260 3044 annotation2210193 1 BBa_K559011 range2210193 1 1 1251 BBa_J23116 1 BBa_J23116 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false false _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K112808 1 BBa_K112808 Enterobacteria phage T4 Lysis Device - no promoter 2008-10-31T12:00:00Z 2015-05-08T01:09:19Z Pubmed Accession Number: NC_000866 Enterobacteria phage T4 This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device. false true _224_ 0 3025 171 It's complicated true [Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin. true Jin Huh component1999023 1 BBa_K112806 component1999021 1 BBa_K112805 component1999026 1 BBa_B0012 component1999033 1 BBa_B0010 component1999024 1 BBa_B0010 component1999032 1 BBa_K112807 component1999030 1 BBa_J23116 annotation1999030 1 BBa_J23116 range1999030 1 1344 1378 annotation1999021 1 BBa_K112805 range1999021 1 1 676 annotation1999023 1 BBa_K112806 range1999023 1 685 1198 annotation1999024 1 BBa_B0010 range1999024 1 1207 1286 annotation1999032 1 BBa_K112807 range1999032 1 1387 1697 annotation1999026 1 BBa_B0012 range1999026 1 1295 1335 annotation1999033 1 BBa_B0010 range1999033 1 1706 1785 BBa_J23116_sequence 1 ttgacagctagctcagtcctagggactatgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K112805_sequence 1 cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataatta BBa_K912029_sequence 1 tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatgtactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K112808_sequence 1 cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K112807_sequence 1 gataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaat BBa_K559011_sequence 1 tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatg BBa_K112806_sequence 1 atacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z