LOCUS BBa_K912031 4515 bp DNA linear 22-Nov-2024 DEFINITION pH Inducible Lysis System(m) ACCESSION BBa_K912031 VERSION BBa_K912031.1 FEATURES Location/Qualifiers misc_feature 1249..1251 /label=gadC Start promoter 141..158 /label=PgadR CDS 275..1105 /label=gadR promoter 1163..1181 /label=Pgad RBS 1237..1245 /label=RBS_gadC RBS 264..269 /label=RBS_gadR promoter 1..1251 /label=BBa_K559011 CDS 1276..1932 /label=T4 holin CDS 1260..1935 /label=BBa_K112805 CDS 1960..2454 /label=T4 Endolysin CDS 1944..2457 /label=BBa_K112806 stem_loop 2477..2520 /label=stem_loop misc_feature 2466..2545 /label=BBa_B0010 terminator 2466..2545 /label=BBa_B0010 stem_loop 2561..2580 /label=T7 TE misc_feature 2587..2587 /label=stop polyA_site 2581..2594 /label=polya misc_feature 2554..2594 /label=BBa_B0012 terminator 2554..2594 /label=BBa_B0012 promoter 2603..2637 /label=BBa_J23116 CDS 2660..2953 /label=T4 antiholin CDS 2646..2956 /label=BBa_K112807 stem_loop 2976..3019 /label=stem_loop misc_feature 2965..3044 /label=BBa_B0010 terminator 2965..3044 /label=BBa_B0010 misc_feature 1260..3044 /label=BBa_K112808 misc_feature 1..3044 /label=BBa_K912029 promoter 3053..3087 /label=BBa_J23110 CDS 3094..4515 /label=BBa_K912005 misc_feature 3053..4515 /label=BBa_K912016 ORIGIN 1 tgagcgttgt ataagctttt atgtctttct atatcaactt ttaatagaaa tataaagtaa 61 tataaatgtt tttataataa attatgtgag atatattttt ttgtccgtac tggtatagat 121 ttgacgatta agtcttaaat aagttataat ctcaattgcg taatttctta aatacagaaa 181 taacaactac attggtagac tgattaaaaa gtgtacttga tgaactgtta taaaccttaa 241 aaaaataaaa ataatagttt gggggatgtt aaagatgtat aaaaaatatg gagattgttt 301 taaaaagttg cgaaaccaaa agaatttagg gttatcatac tttagtaaac ttggaataga 361 ccgttcaaat atatcaagat ttgaacatgg aaaatgtatg atgagttttg agcgtataga 421 tttgatgtta gaagaaatgc aagttccgtt atctgagtac gaattgattg taaataattt 481 tatgccgaat ttccaagaat tttttatatt agaattggaa aaagctgaat ttagccaaaa 541 tcgagataaa ataaaagagt tgtattctga ggtcaaagaa acggggaatc atttactgac 601 ggttaccgtg aaaacgaagc ttgggaatat aagtcagaca gaagttaaag aaattgaagc 661 ttatctttgc aatattgaag agtggggata ttttgaactt actttatttt attttgtatc 721 tgattatctc aatgtcaatc aattagaatt gctgcttttt aattttgata aaagatgtga 781 aaattactgt agagtcttaa aatatagaag gagactattg caaatagcct ataaaagtgt 841 tgcgatatac gcggctaaag gagaaagaaa aaaagccgaa aatattttag aaatgactaa 901 aaaatatcga actgtgggag tcgatttata ttcagaagta ttaagacatc ttgctagagc 961 tatcattatt tttaattttg aaaatgcaga gattggggaa gaaaaaataa attatgctct 1021 tgagattttg gaagaatttg gaggaaagaa gataaaagaa ttttatcaga ataaaatgga 1081 aaagtatttg aaaaggtcaa tttagtctct tttgagctgt tgctttaaag caacagctca 1141 aaagagattt tctttattcc agagcatata ctagagggtg aagataggtt gtctgaagca 1201 ttataacttg tcttttaaaa aattcaatca taaatataag gaggtatgat gtactagagc 1261 ttaaaaggag ggtctatggc agcacctaga atatcatttt cgccctctga tattctattt 1321 ggtgttctcg atcgcttgtt caaagataac gctaccggga aggttcttgc ttcccgggta 1381 gctgtcgtaa ttcttttgtt tataatggcg attgtttggt ataggggaga tagtttcttt 1441 gagtactata agcaatcaaa gtatgaaaca tacagtgaaa ttattgaaaa ggaaagaact 1501 gcacgctttg aatctgtcgc cctggaacaa ctccagatag ttcatatatc atctgaggca 1561 gactttagtg cggtgtattc tttccgccct aaaaacttaa actattttgt tgatattata 1621 gcatacgaag gaaaattacc ttcaacaata agtgaaaaat cacttggagg atatcctgtt 1681 gataaaacta tggatgaata tacagttcat ttaaatggac gtcattatta ttccaactca 1741 aaatttgctt ttttaccaac taaaaagcct actcccgaaa taaactacat gtacagttgt 1801 ccatatttta atttggataa tatctatgct ggaacgataa ccatgtactg gtatagaaat 1861 gatcatataa gtaatgaccg ccttgaatca atatgtgctc aggcggccag aatattagga 1921 agggctaaat aattatacta gagatactta ggaggtatta tgaatatatt tgaaatgtta 1981 cgtatagatg aaggtcttag acttaaaatc tataaagaca cagaaggcta ttacactatt 2041 ggcatcggtc atttgcttac aaaaagtcca tcacttaatg ctgctaaatc tgaattagat 2101 aaagctattg ggcgtaattg caatggtgta attacaaaag atgaggctga aaaactcttt 2161 aatcaggatg ttgatgctgc tgttcgcgga atcctgagaa atgctaaatt aaaaccggtt 2221 tatgattctc ttgatgcggt tcgtcgctgt gcattgatta atatggtttt ccaaatggga 2281 gaaaccggtg tggcaggatt tactaactct ttacgtatgc ttcaacaaaa acgctgggat 2341 gaagcagcag ttaacttagc taaaagtaga tggtataatc aaacacctaa tcgcgcaaaa 2401 cgagtcatta caacgtttag aactggcact tgggacgcgt ataaaaatct ataaagctac 2461 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2521 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2581 tttctgcgtt tatatactag agttgacagc tagctcagtc ctagggacta tgctagctac 2641 tagaggatag gaggccttta tggccttaaa agcaacagca ctttttgcca tgctaggatt 2701 gtcatttgtt ttatctccat cgattgaagc gaatgtcgat cctcattttg ataaatttat 2761 ggaatctggt attaggcacg tttatatgct ttttgaaaat aaaagcgtag aatcgtctga 2821 acaattctat agttttatga gaacgaccta taaaaatgac ccgtgctctt ctgattttga 2881 atgtatagag cgaggcgcgg agatggcaca atcatacgct agaattatga acattaaatt 2941 ggagactgaa tgaaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 3001 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtttacggc 3061 tagctcagtc ctaggtacaa tgctagctac tagatgaaaa ctgatactcc ctctttagaa 3121 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat 3181 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt tgggctggca 3241 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta 3301 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg 3361 gcgatgtttg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc 3421 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtattgccg 3481 gtgaagttct ttggcgggct ggggacactc ggcggaggca tggtgttggg gcgcgaaggg 3541 ccaaccgtgc agatcggcgg taacattggc cgtatggtgc ttgatatttt ccgcctgaaa 3601 ggtgacgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc 3661 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc 3721 tatacgttaa tttcgattaa agcggtattt attggtgtca ttatgtcgac cattatgtac 3781 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt 3841 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt 3901 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc 3961 aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca 4021 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc 4081 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact ctgcttctct 4141 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc 4201 gctttcggaa tggttgccgt tgagctgttt ccgcaatatc accttgaggc ggggacgttt 4261 gctattgccg gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc 4321 attctggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt 4381 cttggcgcaa cactattagc gcaatttacc ggcgggaaac cgctatactc ggcgattctt 4441 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc 4501 agcgagaata cttga //