BBa_K912034
type: ComponentDefinition
persistent identity: BBa K912034
display id: BBa_K912034
version: 1
was generated by: iGEM to SBOL conversion
description: pH Inducible GFP system (ns)
created: 2012-10-02T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: GFP:registry CP/RBS: registry clc-ec1: PCR from genome Pgad: PCR from vector from CUHK 2011 team
owned by: james
owned by: myers
mutable description: This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector. This particular part was constructed in order to test the to make sure that the Pgad/clc system works correctly. We hoped that it would be easier to see GFP for assay purposes. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K912033 The original device] is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1177_
m user id: 0
owner id: 12986
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: None
star: false
creator: Yuan Tao
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K912005
component: BBa_J23111
component: BBa_E0040
component: BBa_K559011
sequence annotation: BBa_K559011
sequence annotation: BBa_E0040
sequence annotation: BBa_J23111
sequence annotation: BBa_K912005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K912034_sequence/1

BBa_B0015
type: Component
persistent identity: component2210442
display id: component2210442
version: 1
top level: BBa_K912034
definition: BBa_B0015
access: public

BBa_K912005
type: Component
persistent identity: component2210435
display id: component2210435
version: 1
top level: BBa_K912034
definition: BBa_K912005
access: public

BBa_J23111
type: Component
persistent identity: component2210434
display id: component2210434
version: 1
top level: BBa_K912034
definition: BBa_J23111
access: public

BBa_E0040
type: Component
persistent identity: component2210433
display id: component2210433
version: 1
top level: BBa_K912034
definition: GFP
access: public

BBa_K559011
type: Component
persistent identity: component2210431
display id: component2210431
version: 1
top level: BBa_K912034
definition: Pgad
access: public

BBa_K559011
type: SequenceAnnotation
persistent identity: annotation2210431
display id: annotation2210431
version: 1
top level: BBa_K912034
location: https://synbiohub.org/public/igem/BBa_K912034/annotation2210431/range2210431/1
component: BBa_K559011

https://synbiohub.org/public/igem/BBa_K912034/annotation2210431/range2210431/1
type: Range
persistent identity: range2210431
display id: range2210431
version: 1
top level: BBa_K912034
direction: direction/reverse
start: 1
end: 1251
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2210433
display id: annotation2210433
version: 1
top level: BBa_K912034
location: https://synbiohub.org/public/igem/BBa_K912034/annotation2210433/range2210433/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K912034/annotation2210433/range2210433/1
type: Range
persistent identity: range2210433
display id: range2210433
version: 1
top level: BBa_K912034
direction: direction/reverse
start: 1258
end: 1977
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation2210434
display id: annotation2210434
version: 1
top level: BBa_K912034
location: https://synbiohub.org/public/igem/BBa_K912034/annotation2210434/range2210434/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_K912034/annotation2210434/range2210434/1
type: Range
persistent identity: range2210434
display id: range2210434
version: 1
top level: BBa_K912034
direction: direction/reverse
start: 1986
end: 2020
orientation: inline

BBa_K912005
type: SequenceAnnotation
persistent identity: annotation2210435
display id: annotation2210435
version: 1
top level: BBa_K912034
location: https://synbiohub.org/public/igem/BBa_K912034/annotation2210435/range2210435/1
component: BBa_K912005

https://synbiohub.org/public/igem/BBa_K912034/annotation2210435/range2210435/1
type: Range
persistent identity: range2210435
display id: range2210435
version: 1
top level: BBa_K912034
direction: direction/reverse
start: 2027
end: 3448
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2210442
display id: annotation2210442
version: 1
top level: BBa_K912034
location: https://synbiohub.org/public/igem/BBa_K912034/annotation2210442/range2210442/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K912034/annotation2210442/range2210442/1
type: Range
persistent identity: range2210442
display id: range2210442
version: 1
top level: BBa_K912034
direction: direction/reverse
start: 3457
end: 3585
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

Pgad
type: ComponentDefinition
persistent identity: BBa K559011
display id: BBa_K559011
version: 1
was generated by: iGEM to SBOL conversion
description: Pgad - Intracellular Chloride-Sensing Cassette
created: 2011-10-27T11:00:00Z
modified: 2015-05-08T01:12:41Z
mutable provenance: The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
owned by: james
owned by: myers
mutable description: Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _727_
m user id: 0
owner id: 6123
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
star: false
creator: Jacky, Fong Chuen, Loo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: PgadR
sequence annotation: RBS_gadR
sequence annotation: gadR
sequence annotation: Pgad
sequence annotation: RBS_gadC
sequence annotation: gadC Start
sequence: https://synbiohub.org/public/igem/BBa_K559011_sequence/1

PgadR
type: SequenceAnnotation
persistent identity: annotation2160937
display id: annotation2160937
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K559011/annotation2160937/range2160937/1
type: Range
persistent identity: range2160937
display id: range2160937
version: 1
top level: Pgad
direction: direction/forward
start: 141
end: 158
orientation: inline

RBS_gadR
type: SequenceAnnotation
persistent identity: annotation2160941
display id: annotation2160941
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K559011/annotation2160941/range2160941/1
type: Range
persistent identity: range2160941
display id: range2160941
version: 1
top level: Pgad
direction: direction/forward
start: 264
end: 269
orientation: inline

gadR
type: SequenceAnnotation
persistent identity: annotation2160938
display id: annotation2160938
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K559011/annotation2160938/range2160938/1
type: Range
persistent identity: range2160938
display id: range2160938
version: 1
top level: Pgad
direction: direction/forward
start: 275
end: 1105
orientation: inline

Pgad
type: SequenceAnnotation
persistent identity: annotation2160939
display id: annotation2160939
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K559011/annotation2160939/range2160939/1
type: Range
persistent identity: range2160939
display id: range2160939
version: 1
top level: Pgad
direction: direction/forward
start: 1163
end: 1181
orientation: inline

RBS_gadC
type: SequenceAnnotation
persistent identity: annotation2160940
display id: annotation2160940
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K559011/annotation2160940/range2160940/1
type: Range
persistent identity: range2160940
display id: range2160940
version: 1
top level: Pgad
direction: direction/forward
start: 1237
end: 1245
orientation: inline

gadC Start
type: SequenceAnnotation
persistent identity: annotation2160936
display id: annotation2160936
version: 1
top level: Pgad
location: https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K559011/annotation2160936/range2160936/1
type: Range
persistent identity: range2160936
display id: range2160936
version: 1
top level: Pgad
direction: direction/forward
start: 1249
end: 1251
orientation: inline

BBa_K912005
type: ComponentDefinition
persistent identity: BBa K912005
display id: BBa_K912005
version: 1
was generated by: iGEM to SBOL conversion
description: ClC-ec1 chloride antiporter
created: 2012-07-08T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: http://ecocyc.org/ECOLI/sequence-delimited?chromosome=COLI-K12&object=EG12331&up=0&down=0&left=175107&right=176528 genomic sequence.
owned by: james
owned by: myers
mutable description: The ClC-ec1 chloride antiporter pumps protons out of the cell and chloride ions into the cell in acidic conditions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1177_
m user id: 0
owner id: 12923
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In progress.
star: false
creator: Grant Nicholas
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K912005_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K912034_sequence/1
type: Sequence
persistent identity: BBa K912034 sequence
display id: BBa_K912034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatgtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagttgacggctagctca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23111_sequence/1
type: Sequence
persistent identity: BBa J23111 sequence
display id: BBa_J23111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K559011_sequence/1
type: Sequence
persistent identity: BBa K559011 sequence
display id: BBa_K559011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgagcgttgtataagcttttatgtctttctatatcaacttttaatagaaatataaagtaatataaatgtttttataataaattatgtgagatatatttttttgtccgtactggtatagatttgacgattaagtcttaaataagttataatctcaattgcgtaatttcttaaatacagaaataacaactacattggtagactgattaaaaagtgtacttgatgaactgttataaaccttaaaaaaataaaaataatagtttgggggatgttaaagatgtataaaaaatatggagattgttttaaaaagttgcgaaaccaaaagaatttagggttatcatactttagtaaacttggaatagaccgttcaaatatatcaagatttgaacatggaaaatgtatgatgagttttgagcgtatagatttgatgttagaagaaatgcaagttccgttatctgagtacgaattgattgtaaataattttatgccgaatttccaagaattttttatattagaattggaaaaagctgaatttagccaaaatcgagataaaataaaagagttgtattctgaggtcaaagaaacggggaatcatttactgacggttaccgtgaaaacgaagcttgggaatataagtcagacagaagttaaagaaattgaagcttatctttgcaatattgaagagtggggatattttgaacttactttattttattttgtatctgattatctcaatgtcaatcaattagaattgctgctttttaattttgataaaagatgtgaaaattactgtagagtcttaaaatatagaaggagactattgcaaatagcctataaaagtgttgcgatatacgcggctaaaggagaaagaaaaaaagccgaaaatattttagaaatgactaaaaaatatcgaactgtgggagtcgatttatattcagaagtattaagacatcttgctagagctatcattatttttaattttgaaaatgcagagattggggaagaaaaaataaattatgctcttgagattttggaagaatttggaggaaagaagataaaagaattttatcagaataaaatggaaaagtatttgaaaaggtcaatttagtctcttttgagctgttgctttaaagcaacagctcaaaagagattttctttattccagagcatatactagagggtgaagataggttgtctgaagcattataacttgtcttttaaaaaattcaatcataaatataaggaggtatgatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K912005_sequence/1
type: Sequence
persistent identity: BBa K912005 sequence
display id: BBa_K912005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaactgatactccctctttagaaacaccgcaggccgcgcgcctgcgacgcagacaactgattcgccaacttcttgagcgcgataaaaccccgttagccattttgtttatggcggcagtcgtcggcacgcttgttgggctggcagcggttgcttttgacaaaggtgtcgcctggttgcagaaccaacgtatgggggcgctggtacatactgctgataattatccgcttctgttaaccgtcgcttttctctgttcggcggtgctggcgatgtttggctactttttggtgcgcaaatacgcgccggaagcaggtggttcggggatcccggaaattgaaggggcgctggaagatcaacgtcccgttcgctggtggcgtgtattgccggtgaagttctttggcgggctggggacactcggcggaggcatggtgttggggcgcgaagggccaaccgtgcagatcggcggtaacattggccgtatggtgcttgatattttccgcctgaaaggtgacgaagctcgccatacgctgctggcaaccggtgctgctgcggggctggctgcggcctttaacgcgccgctggcgggtattttgtttattatcgaagagatgcgtccgcagtttcgctatacgttaatttcgattaaagcggtatttattggtgtcattatgtcgaccattatgtaccggatttttaatcatgaagttgcgttgattgacgtcggtaaactttctgatgcgccgcttaatacgctgtggctttatctgatcctcggtattatttttggcattttcggccctatttttaataaatgggtgctggggatgcaggatttgctgcaccgtgtgcacggcggcaatattaccaaatgggtgctaatgggcggtgcgattggcggtctgtgtggattgctggggtttgtggcaccagcaacgtcgggcggcggttttaacctgattcctatcgctaccgcggggaatttcagcatgggaatgctggtgtttatcttcgtcgcgcgggtcattaccaccttactctgcttctcttccggcgcgccgggcggtatttttgccccgatgctggcgctgggtactgtgctgggaaccgctttcggaatggttgccgttgagctgtttccgcaatatcaccttgaggcggggacgtttgctattgccggaatgggggcattactggcggcatctattcgcgcgccgttaacggggatcattctggttctggagatgaccgataactaccagctcattttgccaatgattattaccggtcttggcgcaacactattagcgcaatttaccggcgggaaaccgctatactcggcgattcttgcgcgcacgctggcaaaacaggaagctgagcaactggcgcgaagcaaggccgcatcagccagcgagaatacttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z