LOCUS BBa_K912037 3585 bp DNA linear 25-Nov-2024 DEFINITION pH Inducible GFP system (w) ACCESSION BBa_K912037 VERSION BBa_K912037.1 FEATURES Location/Qualifiers misc_feature 1249..1251 /label=gadC Start promoter 141..158 /label=PgadR CDS 275..1105 /label=gadR promoter 1163..1181 /label=Pgad RBS 1237..1245 /label=RBS_gadC RBS 264..269 /label=RBS_gadR promoter 1..1251 /label=BBa_K559011 CDS 1258..1977 /label=GFP protein CDS 1258..1977 /label=BBa_E0040 promoter 1986..2020 /label=BBa_J23114 CDS 2027..3448 /label=BBa_K912005 stem_loop 3468..3511 /label=stem_loop misc_feature 3457..3536 /label=BBa_B0010 terminator 3457..3536 /label=BBa_B0010 stem_loop 3552..3571 /label=T7 TE misc_feature 3578..3578 /label=stop polyA_site 3572..3585 /label=polya misc_feature 3545..3585 /label=BBa_B0012 terminator 3545..3585 /label=BBa_B0012 ORIGIN 1 tgagcgttgt ataagctttt atgtctttct atatcaactt ttaatagaaa tataaagtaa 61 tataaatgtt tttataataa attatgtgag atatattttt ttgtccgtac tggtatagat 121 ttgacgatta agtcttaaat aagttataat ctcaattgcg taatttctta aatacagaaa 181 taacaactac attggtagac tgattaaaaa gtgtacttga tgaactgtta taaaccttaa 241 aaaaataaaa ataatagttt gggggatgtt aaagatgtat aaaaaatatg gagattgttt 301 taaaaagttg cgaaaccaaa agaatttagg gttatcatac tttagtaaac ttggaataga 361 ccgttcaaat atatcaagat ttgaacatgg aaaatgtatg atgagttttg agcgtataga 421 tttgatgtta gaagaaatgc aagttccgtt atctgagtac gaattgattg taaataattt 481 tatgccgaat ttccaagaat tttttatatt agaattggaa aaagctgaat ttagccaaaa 541 tcgagataaa ataaaagagt tgtattctga ggtcaaagaa acggggaatc atttactgac 601 ggttaccgtg aaaacgaagc ttgggaatat aagtcagaca gaagttaaag aaattgaagc 661 ttatctttgc aatattgaag agtggggata ttttgaactt actttatttt attttgtatc 721 tgattatctc aatgtcaatc aattagaatt gctgcttttt aattttgata aaagatgtga 781 aaattactgt agagtcttaa aatatagaag gagactattg caaatagcct ataaaagtgt 841 tgcgatatac gcggctaaag gagaaagaaa aaaagccgaa aatattttag aaatgactaa 901 aaaatatcga actgtgggag tcgatttata ttcagaagta ttaagacatc ttgctagagc 961 tatcattatt tttaattttg aaaatgcaga gattggggaa gaaaaaataa attatgctct 1021 tgagattttg gaagaatttg gaggaaagaa gataaaagaa ttttatcaga ataaaatgga 1081 aaagtatttg aaaaggtcaa tttagtctct tttgagctgt tgctttaaag caacagctca 1141 aaagagattt tctttattcc agagcatata ctagagggtg aagataggtt gtctgaagca 1201 ttataacttg tcttttaaaa aattcaatca taaatataag gaggtatgat gtactagatg 1261 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat 1321 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa 1381 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc 1441 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat 1501 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa 1561 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat 1621 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg 1681 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc 1741 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat 1801 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg 1861 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt 1921 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac 1981 tagagtttat ggctagctca gtcctaggta caatgctagc tactagatga aaactgatac 2041 tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact 2101 tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct 2161 tgttgggctg gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat 2221 gggggcgctg gtacatactg ctgataatta tccgcttctg ttaaccgtcg cttttctctg 2281 ttcggcggtg ctggcgatgt ttggctactt tttggtgcgc aaatacgcgc cggaagcagg 2341 tggttcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcccg ttcgctggtg 2401 gcgtgtattg ccggtgaagt tctttggcgg gctggggaca ctcggcggag gcatggtgtt 2461 ggggcgcgaa gggccaaccg tgcagatcgg cggtaacatt ggccgtatgg tgcttgatat 2521 tttccgcctg aaaggtgacg aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg 2581 gctggctgcg gcctttaacg cgccgctggc gggtattttg tttattatcg aagagatgcg 2641 tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggtg tcattatgtc 2701 gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc 2761 tgatgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt 2821 cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg 2881 cggcaatatt accaaatggg tgctaatggg cggtgcgatt ggcggtctgt gtggattgct 2941 ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc 3001 ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt 3061 actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac 3121 tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat atcaccttga 3181 ggcggggacg tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc 3241 gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca ttttgccaat 3301 gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga aaccgctata 3361 ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa 3421 ggccgcatca gccagcgaga atacttgata ctagagccag gcatcaaata aaacgaaagg 3481 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 3541 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //