BBa_K914000
1
BBa_K914000
pLac-supD-T
2012-09-17T11:00:00Z
2015-05-08T01:13:45Z
This part is made from the 3 Biobricks previously describe : R0011 - K228001 - B1006
This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.
false
false
_1179_
0
13470
9
It's complicated
true
The promoter is the last part added, meaning that from the previous intermediate (soon available), we will be able to change the promoter easily.
false
Jean Cury
annotation2189151
1
lac O1
range2189151
1
26
42
annotation2189148
1
BBa_R0011
range2189148
1
1
54
annotation2189152
1
-10
range2189152
1
43
48
annotation2189150
1
-35
range2189150
1
20
25
annotation2189155
1
modified thr terminator
range2189155
1
217
238
annotation2189153
1
BBa_K228001
range2189153
1
64
199
annotation2189154
1
BBa_B1006 modified
range2189154
1
208
241
annotation2189149
1
lac O1
range2189149
1
3
19
BBa_K914000_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagcgccgcaaaccccgcttcggcggggtttcgccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z