LOCUS BBa_K914008 861 bp DNA linear 24-Nov-2024 DEFINITION Meganuclease I-SceI controlled by pRha ACCESSION BBa_K914008 VERSION BBa_K914008.1 FEATURES Location/Qualifiers promoter 1..122 /label=pRha promoter 1..122 /label=BBa_K914003 RBS 131..143 /label=RBS-3\Weak RBS 137..140 /label=RBS misc_feature 131..143 /label=BBa_B0032 RBS 131..143 /label=BBa_B0032 CDS 150..861 /label=I-SceI CDS 150..861 /label=BBa_K914006 ORIGIN 1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc 61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg 121 aatactagag tcacacagga aagtactaga tgaaaaacat caaaaaaaac caggtaatga 181 acctgggtcc gaactctaaa ctgctgaaag aatacaaatc ccagctgatc gaactgaaca 241 tcgaacagtt cgaagcaggt atcggtctga tcctgggtga tgcttacatc cgttctcgtg 301 atgaaggtaa aacctactgt atgcagttcg agtggaaaaa caaagcatac atggaccacg 361 tatgtctgct gtacgatcag tgggtactgt ccccgccgca caaaaaagaa cgtgttaacc 421 acctgggtaa cctggtaatc acctggggcg cccagacttt caaacaccaa gctttcaaca 481 aactggctaa cctgttcatc gttaacaaca aaaaaaccat cccgaacaac ctggttgaaa 541 actacctgac cccgatgtct ctggcatact ggttcatgga tgatggtggt aaatgggatt 601 acaacaaaaa ctctaccaac aaatcgatcg tactgaacac ccagtctttc actttcgaag 661 aagtagaata cctggttaag ggtctgcgta acaaattcca actgaactgt tacgtaaaaa 721 tcaacaaaaa caaaccgatc atctacatcg attctatgtc ttacctgatc ttctacaacc 781 tgatcaaacc gtacctgatc ccgcagatga tgtacaaact gccgaacact atctcctccg 841 aaactttcct gaaataataa g //