BBa_K914009 1 BBa_K914009 P1003* Ser133->Amber Codon 2012-09-20T11:00:00Z 2015-05-08T01:13:46Z this part comes from BBa_P1003 The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance. This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer. Actually it would be better with another mutation. false false _1179_ 0 13470 9 It's complicated true I made the single point mutation with a quick change directed mutagenesis kit. false Jean Cury annotation2212600 1 BBa_P1003 range2212600 1 1 967 annotation2212602 1 Ser133->Amber codon range2212602 1 546 546 annotation2212601 1 kanamycin resistance range2212601 1 149 967 BBa_K914009_sequence 1 ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z