BBa_K914009
1
BBa_K914009
P1003* Ser133->Amber Codon
2012-09-20T11:00:00Z
2015-05-08T01:13:46Z
this part comes from BBa_P1003
The single mutation avoid the expression of the P1003 gene, that prevent the expression of a kanamycin resistance.
This mutation is rescue with the tRNA amber suppressor supD (BBa_K914000), or any genotype relative to sup44 . The idea here is to prevent the kanamycin gene resistance in case horizontal gene transfer.
Actually it would be better with another mutation.
false
false
_1179_
0
13470
9
It's complicated
true
I made the single point mutation with a quick change directed mutagenesis kit.
false
Jean Cury
annotation2212600
1
BBa_P1003
range2212600
1
1
967
annotation2212602
1
Ser133->Amber codon
range2212602
1
546
546
annotation2212601
1
kanamycin resistance
range2212601
1
149
967
BBa_K914009_sequence
1
ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattagattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z