BBa_K916001 1 BBa_K916001 TraR-NGFP translational Fusion 2012-09-30T11:00:00Z 2015-05-08T01:13:46Z TraR came from a plasmid (pCF222)provided by Dr. Clay Fuqua at University of Illinois Champaign-Urbana. The NGFP fragment came from a plasmid (pET11a-link-NGFP) provided by Dr. Lynne Regan at Yale University. This a fusion protein containing TraR in its entirety fused to the N terminal fragment of GFP. This is for use with a GFP complementation false false _1181_ 0 12765 9 In stock false TraR was cloned into existing sites in the pET11a-link-NGFP plasmid (Xho1 and BamH1). Because it was a translational fusion, care had to be taken to ensure the protein was in frame. false Joseph Elsherbini annotation2211697 1 TraR range2211697 1 528 1247 annotation2211696 1 XhoI site range2211696 1 521 526 annotation2211698 1 BamHI site range2211698 1 1233 1238 annotation2211699 1 stop range2211699 1 1245 1247 annotation2211694 1 start range2211694 1 3 5 annotation2211695 1 NGFP range2211695 1 3 527 BBa_K916001_sequence 1 atatggctagccatcaccaccatcaccatggcgcgagcaaaggagaagaactcttcactggagttgtcccaattcttgttgaattagatggtgatgttaacggccacaagttctctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctgaagttcatctgcactactggcaaactgcctgttccatggccaacactggtcactactctgtgctatggtgttcaatgcttttcaagatacccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaggaccatcttcttcaaagatgacggcaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgacttcaaggaagatggcaacattctgggacacaaattggaatacaactataactcacacaacgttctcatcatggcagacaaacaaggtggctctggctctggctcgagcatgcagcactggctggacaagctgactgatcttgccgcgatcgaaggcgatgagtgcatcctgaagaccgggctggcggacatcgccgaccatttcggcttcaccggctatgcctaccttcatatccagcacaggcacatcaccgccgttaccaactatcaccgcccaatggcaatcaactacttcgacaagaagttcgaagcgctcgatccggtcgtcaaacgcgcgaggtcccggaagcacatcttcacctggtcgggcgagcacgagcggccgacgctgtcgaaggacgagcgtgccttctatgaccacgcatccgatttcggcatccgctccggcatcacaatacccatcaagaccgccaacggctttatgtcgatgttcacgatggcatcggacaagccggtgatcgatctcgatcgggagatcgatgcagtcgcagccgctgcaaccatcgggcagatccatgcccgcatctcattccttcgcaccacccctaccgcggaagatgccgcatggctcgatccgaaggaggccacctatctgagatggattgccgtcggcaagacgatggaggagatcgccgacgtcgaaggggtcaagtacaacagcgtccgcgtcaagctacgcgaagccatgaagcgcttcgacgtccgcagcaaggcccatcttaccgcgctcgccatccggcggaaactcatctgaggatccggctgctaacaaagcccgaaaggaagctgagttggctgctgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z