BBa_K916002 1 BBa_K916002 TraR-CGFP Translational Fusion 2012-09-30T11:00:00Z 2015-05-08T01:13:46Z TraR came from a plasmid (pCF222)provided by Dr. Clay Fuqua at University of Illinois Champaign-Urbana. The CGFP fragment came from a plasmid (pMRBAD-link-CGFP) provided by Dr. Lynne Regan at Yale University. This a fusion protein containing TraR in its entirety fused to the N terminal fragment of GFP. This is for use with a GFP complementation experiment. false false _1181_ 0 12765 9 In stock false TraR was cloned into existing sites in the pMRBAD-link-NGFP plasmid (NcoI and SphI). Because it was a translational fusion, care had to be taken to ensure the protein was in frame. Also, the pMRBAD-link-CGFP plasmid was designed to use NcoI and AatII, but TraR contained two AatII sites. Cloning into the SphI site instead added two extra amino acids in the linker. false Joseph Elsherbini annotation2207152 1 CGFP range2207152 1 707 1040 annotation2207153 1 stop range2207153 1 1038 1040 annotation2207150 1 TraR range2207150 1 3 706 annotation2211550 1 Sph1 site range2211550 1 708 714 annotation2207151 1 start range2207151 1 3 5 BBa_K916002_sequence 1 ttatggctcagcactggctggacaagctgactgatcttgccgcgatcgaaggcgatgagtgcatcctgaagaccgggctggcggacatcgccgaccatttcggcttcaccggctatgcctaccttcatatccagcacaggcacatcaccgccgttaccaactatcaccgccaatggcaatcaacctacttcgacaagaagttcgaagcgctcgatccggtcgtcaaacgcgcgaggtcccggaagcacatcttcacctggtcgggcgagcacgagcggccgacgctgtcgaaggacgagcgtgccttctatgaccacgcatccgatttcggcatccgctccggcatcacaatacccatcaagaccgccaacggctttatgtcgatgttcacgatggcatcggacaagccggtgatcgatctcgatcgggagatcgatgcagtcgcagccgctgcaaccatcgggcagatccatgcccgcatctcattccttcgcaccacccctaccgcggaagatgccgcatggctcgatccgaaggaggccacctatctgagatggattgccgtcggcaagacgatggaggagatcgccgacgtcgaaggggtcaagtacaacagcgtccgcgtcaagctacgcgaagccatgaagcgcttcgacgtccgcagcaaggcccatcttaccgcgctcgccatccggcggaaactcatcgcatgcaatgggacgtcgggtggaagcggtaagaatggaatcaaagtgaacttcaagacccgccacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactgtacaactaaggatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataacccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z