BBa_K917000 1 BBa_K917000 cscA sucrose hydrolase from E. coli 157:H7 Sakai 2012-06-18T11:00:00Z 2015-05-08T01:13:46Z Escherichia coli O157:H7 strain Sakai The is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene. K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence). false false _1182_ 0 837 163 In stock true Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included. false Chris French annotation2176951 1 start codon range2176951 1 11 13 annotation2176952 1 stop codon range2176952 1 1442 1444 annotation2176953 1 RBS range2176953 1 1 2 BBa_K917000_sequence 1 gatgataaaaatgacgcaatctcgattgcatgcggcgcaaaacgcactagcaaaacttcacgagcgccgaggtaacactttctatccccattttcacctcgcgcctcctgccgggtggatgaacgatccaaacggcctgatctggtttaacgatcgttatcacgcgttttatcaacatcacccaatgagtgaacactgggggccaatgcactggggacatgccaccagcgacgatatgatccactggcagcatgagcctattgcgctagcgccaggagacgagaatgacaaagatgggtgtttttcaggtagtgctgtcgatgacaatggtgtcctctcacttatctacaccggacacgtctggctcgatggtgcaggtaatgacgatgcaattcgcgaagtacaatgtctggctaccagtcgggatggtattcatttcgagaaacagggtgtgatcctcactccaccagaaggaatcatgcacttccgcgatcctaaagtgtggcgtgaagccgacacatggtggatggtagtcggggcgaaagacccaggcaacactgggcagatcctgctttatcgcggcagttcattacgtgaatggactttcgatcgcgtactggcccacgctgatgcgggtgaaagctatatgtgggaatgtccggactttttcagccttggcgatcagcattatctgatgttttccccgcagggaatgaatgccgagggatacagttaccgaaatcgctttcaaagtggcgtaatacccggaatgtggtcgccaggacgactttttgcacaatccgggcattttactgaacttgataacgggcatgacttttatgcaccacaaagctttgtagcgaaggatggtcggcgtattgttatcggctggatggatatgtgggaatcgccaatgccctcaaaacgtgaaggctgggcaggctgcatgacgctggcgcgcgagctatcagagagcaatggcaaacttctacaacgcccggtacacgaagctgagtcgttacgccagcagcatcaatctatctctccccgcacaatcagcaataaatatgttttgcaggaaaacgcgcaagcagttgagattcagttgcagtgggcgctgaagaacagtgatgccgaacattacggattacagctcggcactggaatgcggctgtatattgataaccaatctgagcgacttgttttgtggcggtattacccacacgagaatttagacggctaccgtagtattcccctcccgcagcgtgacacgctcgccctaaggatatttatcgatacatcatccgtggaagtatttattaacgacggggaagcggtgatgagtagtcgaatctatccgcagccagaagaacgggaactgtcgctttatgcctcccacggagtggctgtggtgcaacatggagcactctggcaactgggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z