LOCUS       BBa_K917001             2221 bp    DNA     linear       03-Jun-2024
DEFINITION  ars promoter plus cscA sucrose assimilation gene
ACCESSION   BBa_K917001
VERSION     BBa_K917001.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     RBS             527..530
                     /label=rbs
     CDS             539..769
                     /label=lacZ'
     misc_feature    527..769
                     /label=BBa_J33202
     misc_feature    1..769
                     /label=BBa_J33203
     misc_feature    788..790
                     /label=start codon
     misc_feature    2219..2221
                     /label=stop codon
     RBS             778..779
                     /label=RBS
     misc_feature    778..2221
                     /label=BBa_K917000
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagaggagg aaacagctat
      541 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      601 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      661 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      721 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagaggat
      781 gataaaaatg acgcaatctc gattgcatgc ggcgcaaaac gcactagcaa aacttcacga
      841 gcgccgaggt aacactttct atccccattt tcacctcgcg cctcctgccg ggtggatgaa
      901 cgatccaaac ggcctgatct ggtttaacga tcgttatcac gcgttttatc aacatcaccc
      961 aatgagtgaa cactgggggc caatgcactg gggacatgcc accagcgacg atatgatcca
     1021 ctggcagcat gagcctattg cgctagcgcc aggagacgag aatgacaaag atgggtgttt
     1081 ttcaggtagt gctgtcgatg acaatggtgt cctctcactt atctacaccg gacacgtctg
     1141 gctcgatggt gcaggtaatg acgatgcaat tcgcgaagta caatgtctgg ctaccagtcg
     1201 ggatggtatt catttcgaga aacagggtgt gatcctcact ccaccagaag gaatcatgca
     1261 cttccgcgat cctaaagtgt ggcgtgaagc cgacacatgg tggatggtag tcggggcgaa
     1321 agacccaggc aacactgggc agatcctgct ttatcgcggc agttcattac gtgaatggac
     1381 tttcgatcgc gtactggccc acgctgatgc gggtgaaagc tatatgtggg aatgtccgga
     1441 ctttttcagc cttggcgatc agcattatct gatgttttcc ccgcagggaa tgaatgccga
     1501 gggatacagt taccgaaatc gctttcaaag tggcgtaata cccggaatgt ggtcgccagg
     1561 acgacttttt gcacaatccg ggcattttac tgaacttgat aacgggcatg acttttatgc
     1621 accacaaagc tttgtagcga aggatggtcg gcgtattgtt atcggctgga tggatatgtg
     1681 ggaatcgcca atgccctcaa aacgtgaagg ctgggcaggc tgcatgacgc tggcgcgcga
     1741 gctatcagag agcaatggca aacttctaca acgcccggta cacgaagctg agtcgttacg
     1801 ccagcagcat caatctatct ctccccgcac aatcagcaat aaatatgttt tgcaggaaaa
     1861 cgcgcaagca gttgagattc agttgcagtg ggcgctgaag aacagtgatg ccgaacatta
     1921 cggattacag ctcggcactg gaatgcggct gtatattgat aaccaatctg agcgacttgt
     1981 tttgtggcgg tattacccac acgagaattt agacggctac cgtagtattc ccctcccgca
     2041 gcgtgacacg ctcgccctaa ggatatttat cgatacatca tccgtggaag tatttattaa
     2101 cgacggggaa gcggtgatga gtagtcgaat ctatccgcag ccagaagaac gggaactgtc
     2161 gctttatgcc tcccacggag tggctgtggt gcaacatgga gcactctggc aactgggtta
     2221 a
//