LOCUS       BBa_K917002             2377 bp    DNA     linear       26-Dec-2024
DEFINITION  lac promoter plus RFP and sacB
ACCESSION   BBa_K917002
VERSION     BBa_K917002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..932
                     /label=BBa_E1010
     RBS             941..943
                     /label=RBS
     CDS             953..2374
                     /label=Coding sequence
     misc_feature    1151..1156
                     /label=Removed EcoRI site
     misc_feature    941..2377
                     /label=BBa_K322921
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag gagacatgaa cgatgaacat
      961 caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc tggcaggagg
     1021 cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa catacggcat
     1081 ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa atgaaaaata
     1141 tcaagttcct gagttcgatt cgtccacaat taaaaatatc tcttctgcaa aaggcctgga
     1201 cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact atcacggcta
     1261 ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat cgatttacat
     1321 gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg gccgcgtctt
     1381 taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa cacaagaatg
     1441 gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca ctgatttctc
     1501 cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat cagcatcaga
     1561 cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg gtgacggaaa
     1621 aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag gcgacaacca
     1681 tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag tatttgaagc
     1741 aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca aagcatacta
     1801 tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa
     1861 acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg atgattacac
     1921 actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg aaattgaacg
     1981 cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc gcggatcaaa
     2041 aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg tttctaattc
     2101 tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa tggatcttga
     2161 tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga aaggaaacaa
     2221 tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac aatcaacgtt
     2281 tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca aagacagcat
     2341 ccttgaacaa ggacaattaa cagttaacaa ataataa
//