LOCUS       BBa_K917005             1279 bp    DNA     linear       27-Jun-2024
DEFINITION  lac promoter plus nitroreductase (nsfI) gene from Enterobacter 
            cloacae
ACCESSION   BBa_K917005
VERSION     BBa_K917005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    623..625
                     /label=start codon
     misc_feature    1274..1276
                     /label=stop codon 
     RBS             613..619
                     /label=RBS
     misc_feature    609..1279
                     /label=BBa_K917004
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagca ccaggagttg ttatggatat catttctgtc gccctgaaac gccactctac
      661 caaggcgttc gacgcaagca aaaaactgac cgcggaagaa gcggaaaaaa tcaaaaccct
      721 gctgcaatac agcccgtcca gcaccaactc ccagccgtgg cacttcattg tagccagcac
      781 cgaggaagga aaagcgcgcg tggcgaagtc cgctgcgggc acctatgtgt tcaacgaacg
      841 caaaatgctg gatgcttccc acgtggtggt gttctgcgcg aaaaccgcga tggatgacgc
      901 ctggctggag cgcgtcgtgg atcaggaaga ggccgatggc cgtttcaaca cgccggaagc
      961 caaagccgca aaccataagg gccgcaccta cttcgccgac atgcaccgcg tggatctgaa
     1021 agatgacgac cagtggatgg cgaagcaggt ttacctgaac gtcggcaact tcctgctggg
     1081 cgtgggcgcg atgggtctgg acgcggtacc aattgaaggt ttcgacgccg ctattctcga
     1141 cgaagagttt ggcctgaaag agaaaggctt caccagcctg gtggtggtac cggttgggca
     1201 ccacagcgtg gaagatttca acgccacgct gccgaaatct cgcctgccgc tgagcacgat
     1261 tgtgaccgag tgctaataa
//