BBa_K917007 1 BBa_K917007 S. oneidensis decaheme cytochrome c electron export system mtrCAB 2012-08-09T11:00:00Z 2015-05-08T01:13:46Z Shewanella oneidensis MR-1 chromosomal DNA This part consists of three genes from the native gene cluster from Shewanella oneidensis MR-1 with native RBSs: MtrA is a decaheme cytochrome c located between inner and outer membrane; MtrB is aa transmembrane beta-barrel responsible for facilitating contact between MtrA and MtrC; MtrC (alternative name: OmcB) is a decaheme cytochrome c attached to the outer membrane. Together these proteins are able to form an extracellular electron export system. (Jensen et al, 2010, PNAS vol. 107 no. 45 19213-19218; doi: 10.1073/pnas.1009645107) false false _1182_ 0 13804 9 It's complicated true Genes were obtained by PCR amplification of S. oneidensis MR-1 gene cluster. MtrA and MtrC contained PstI restriction sites that had to be removed by site-directed mutagensis false Jakub Krakowiak annotation2179457 1 PstI site G>A range2179457 1 2191 2191 annotation2179455 1 Stop codon range2179455 1 5230 5232 annotation2179454 1 Start codon range2179454 1 41 43 annotation2179458 1 RBS range2179458 1 31 36 annotation2179456 1 PstI site T>A range2179456 1 1834 1834 BBa_K917007_sequence 1 catagcggttaagcaatgccaaacctatgcagggaaaaaaatgatgaacgcacaaaaatcaaaaatcgcactgctgctcgcagcaagtgccgtcacaatggccttaaccggctgtggtggaagcgatggtaataacggcaatgatggtagtgatggtggtgagccagcaggtagcatccagacgttaaacctagatatcactaaagtaagctatgaaaatggtgcacctatggtcactgttttcgccactaacgaagccgacatgccagtgattggtctcgcaaatttagaaatcaaaaaagcactgcaattaataccggaaggggcgacaggcccaggtaatagcgctaactggcaaggcttaggctcatcaaagagctatgtcgataataaaaacggtagctatacctttaaattcgacgccttcgatagtaataaggtctttaatgctcaattaacgcaacgctttaacgttgtttctgctgcgggtaaattagcagacggaacgaccgttcccgttgccgaaatggttgaagatttcgacggccaaggtaatgcgccgcaatatacaaaaaatatcgttagccacgaagtatgtgcttcttgccacgtagaaggtgaaaagatttatcaccaagctactgaagtcgaaacttgtatttcttgccacactcaagagtttgcggatggtcgcggcaaaccccatgtcgcctttagtcacttaattcacaatgtgcataatgccaacaaagcttggggcaaagacaataaaatccctacagttgcacaaaatattgtccaagataattgccaagtttgtcacgttgaatccgacatgctcaccgaggcaaaaaactggtcacgtattccaacaatggaagtctgttctagctgtcacgtagacatcgattttgctgcgggtaaaggccactctcaacaactcgataactccaactgtatcgcctgccataacagcgactggactgctgagttacacacagccaaaaccaccgcaactaagaacttgattaatcaatacggtatcgagactacctcgacaattaataccgaaactaaagcagccacaattagtgttcaagttgtagatgcgaacggtactgctgttgatctcaagaccatcctgcctaaagtgcaacgcttagagatcatcaccaacgttggtcctaataatgcaaccttaggttatagtggcaaagattcaatatttgcaatcaaaaatggagctcttgatccaaaagctactatcaatgatgctggcaaactggtttataccactactaaagacctcaaacttggccaaaacggcgcagacagcgacacagcatttagctttgtaggttggtcaatgtgttctagcgaaggtaagtttgtagactgtgcagaccctgcatttgatggtgttgatgtaactaagtataccggcatgaaagcggatttagcctttgctactttgtcaggtaaagcaccaagtactcgccacgttgattctgttaacatgacagcctgtgccaattgccacactgctgagttcgaaattcacaaaggcaaacaacatgcaggctttgtgatgacagagcaactatcacacacccaagatgctaacggtaaagcgattgtaggccttgacgcatgtgtgacttgtcatactcctgatggcacctatagctttgccaaccgtggtgcgctagagctaaaactacacaaaaaacacgttgaagatgcctacggcctcattggtggcaattgtgcctcttgtcactcagacttcaaccttgagtctttcaagaagaaaggcgcattgaatactgccgcagcagcagataaaacaggtctatattctacgccgatcactgcaacttgtactacctgtcacacagttggcagccagtacatggtccatacgaaagaaaccctggagtctttcggtgcagttgttgatggcacaaaagatgatgctaccagtgcggcacagtcagaaacctgtttctactgccataccccaacagttgcagatcacactaaagtgaaaatgtaatttgcccaagcagggggagctcgctccccctttcttgaattttgttgggacaaattgggaagcctattatgaagaactgcctaaaaatgaaaaacctactgccggcacttaccatcacaatggcaatgtctgcaattatggcattagtcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagcaagtcgaagccaccttagataagaagtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccataagaaatccgaaaaagtcatggaccttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcctgcaatgtgaggcatgccacggcccactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagcaatcaaccttaagtgccgacaagcaaaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggcggtcaccatgacaatgccgatgttgcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaaacacggaaatggaagtctgtactagctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaaatgggcacaaatgacctgtagcgactgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtgaatgatacctgttattcctgtcacgccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtgtcacttgccacaatcctcacggtagtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtcacgccagcgatggccacgccagcaacgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttactggtggaagaagctgcttaaattgccatagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaaggagacgagaaaatgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcaggtatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacctattccaactccgacagtaacactcaagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcataacattaacttatacgctcaatatcaagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataaggacaatgacgccgcaaatgatatcgccgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacaccgcacaaatgctgatgctgagcatgagttacaaactctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z