BBa_K917008 1 BBa_K917008 S. oneidensis decaheme cytochrome c mtrA 2012-08-09T11:00:00Z 2015-05-08T01:13:46Z Shewanella oneidensis MR-1 chromosomal DNA This part consists of Shewanella oneidensis MR-1 gene mtrA with native RBS. MtrA is a decaheme cytochrome c responsible for electron transport within periplasm and was demonstrated to reduce soluble iron (III) to iron (II). (Jensen et al., 2010, PNAS vol. 107 no. 45 19213-19218; doi: 10.1073/pnas.1009645107) false false _1182_ 0 13804 9 It's complicated true Gene was obtained by PCR amplification of S. oneidensis MR-1 gene. MtrA contains a PstI site that had to be removed by site-directed mutagenesis. false Jakub Krakowiak annotation2179459 1 PstI site G>A range2179459 1 89 89 annotation2179461 1 Stop codon range2179461 1 1022 1024 annotation2179460 1 Start codon range2179460 1 23 25 annotation2179462 1 RBS range2179462 1 13 16 BBa_K917008_sequence 1 gggacaaattgggaagcctattatgaagaactgcctaaaaatgaaaaacctactgccggcacttaccatcacaatggcaatgtctgcaattatggcattagtcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagcaagtcgaagccaccttagataagaagtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccataagaaatccgaaaaagtcatggaccttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcctgcaatgtgaggcatgccacggcccactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagcaatcaaccttaagtgccgacaagcaaaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggcggtcaccatgacaatgccgatgttgcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaaacacggaaatggaagtctgtactagctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaaatgggcacaaatgacctgtagcgactgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtgaatgatacctgttattcctgtcacgccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtgtcacttgccacaatcctcacggtagtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtcacgccagcgatggccacgccagcaacgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttactggtggaagaagctgcttaaattgccatagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z