LOCUS       BBa_K917010             3352 bp    DNA     linear       22-Dec-2024
DEFINITION  Kanamycin resistance+lac promoter+RFP+sacB selection-counterselecti
            on cassette
ACCESSION   BBa_K917010
VERSION     BBa_K917010.1  
FEATURES             Location/Qualifiers
     CDS             149..967
                     /label=kanamycin resistance
     misc_feature    1..967
                     /label=BBa_P1003
     CDS             976..1063
                     /label=end of LacI coding region (inactive)
     misc_feature    976..1175
                     /label=BBa_R0010
     misc_feature    1064..1101
                     /label=CAP binding site
     promoter        1112..1117
                     /label=-35
     promoter        1136..1141
                     /label=-10
     misc_feature    1141..1175
                     /label=LacI binding site
     misc_feature    1148..1148
                     /label=start
     promoter        976..1175
                     /label=BBa_R0010
     misc_feature    1188..1191
                     /label=conserved
     RBS             1184..1195
                     /label=BBa_B0034
     CDS             1202..1876
                     /label=mrfp1
     misc_feature    1883..1907
                     /label=Help:Barcodes
     CDS             1202..1907
                     /label=BBa_E1010
     RBS             1916..1918
                     /label=RBS
     CDS             1928..3349
                     /label=Coding sequence
     misc_feature    2126..2131
                     /label=Removed EcoRI site
     misc_feature    1916..3352
                     /label=BBa_K322921
     misc_feature    976..3352
                     /label=BBa_K917002
ORIGIN
        1 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa
       61 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
      121 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
      181 ccgtccgcgc ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga
      241 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
      301 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg
      361 tctcaactgg ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc
      421 tgatgatgcg tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga
      481 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt
      541 acattcgatt cctgtttgta attgtccttt taacagcgat cgtgtatttc gtcttgctca
      601 ggcgcaatca cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
      661 tggctggcct gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccgga
      721 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
      781 aataggttgt attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat
      841 tctttggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
      901 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
      961 ctaataatac tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1021 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1081 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1141 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
     1201 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     1261 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     1321 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     1381 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     1441 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
     1501 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
     1561 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
     1621 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
     1681 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
     1741 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
     1801 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
     1861 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagaggagac
     1921 atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca gtattaacct ttactaccgc
     1981 actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa
     2041 ggaaacatac ggcatttccc atattacacg ccatgatatg ctgcaaatcc ctgaacagca
     2101 aaaaaatgaa aaatatcaag ttcctgagtt cgattcgtcc acaattaaaa atatctcttc
     2161 tgcaaaaggc ctggacgttt gggacagctg gccattacaa aacgctgacg gcactgtcgc
     2221 aaactatcac ggctaccaca tcgtctttgc attagccgga gatcctaaaa atgcggatga
     2281 cacatcgatt tacatgttct atcaaaaagt cggcgaaact tctattgaca gctggaaaaa
     2341 cgctggccgc gtctttaaag acagcgacaa attcgatgca aatgattcta tcctaaaaga
     2401 ccaaacacaa gaatggtcag gttcagccac atttacatct gacggaaaaa tccgtttatt
     2461 ctacactgat ttctccggta aacattacgg caaacaaaca ctgacaactg cacaagttaa
     2521 cgtatcagca tcagacagct ctttgaacat caacggtgta gaggattata aatcaatctt
     2581 tgacggtgac ggaaaaacgt atcaaaatgt acagcagttc atcgatgaag gcaactacag
     2641 ctcaggcgac aaccatacgc tgagagatcc tcactacgta gaagataaag gccacaaata
     2701 cttagtattt gaagcaaaca ctggaactga agatggctac caaggcgaag aatctttatt
     2761 taacaaagca tactatggca aaagcacatc attcttccgt caagaaagtc aaaaacttct
     2821 gcaaagcgat aaaaaacgca cggctgagtt agcaaacggc gctctcggta tgattgagct
     2881 aaacgatgat tacacactga aaaaagtgat gaaaccgctg attgcatcta acacagtaac
     2941 agatgaaatt gaacgcgcga acgtctttaa aatgaacggc aaatggtacc tgttcactga
     3001 ctcccgcgga tcaaaaatga cgattgacgg cattacgtct aacgatattt acatgcttgg
     3061 ttatgtttct aattctttaa ctggcccata caagccgctg aacaaaactg gccttgtgtt
     3121 aaaaatggat cttgatccta acgatgtaac ctttacttac tcacacttcg ctgtacctca
     3181 agcgaaagga aacaatgtcg tgattacaag ctatatgaca aacagaggat tctacgcaga
     3241 caaacaatca acgtttgcgc caagcttcct gctgaacatc aaaggcaaga aaacatctgt
     3301 tgtcaaagac agcatccttg aacaaggaca attaacagtt aacaaataat aa
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