##gff-version 3
##sequence-region BBa_K917010 1 3352
BBa_K917010	.	engineered_region	1	967	.	+	0	ID=BBa_P1003;Name=BBa_P1003
BBa_K917010	.	CDS	149	967	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
BBa_K917010	.	engineered_region	976	3352	.	+	0	ID=BBa_K917002;Name=BBa_K917002
BBa_K917010	.	ribosome_entry_site	1184	1195	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K917002
BBa_K917010	.	conserved_region	1188	1191	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K917010	.	engineered_region	1916	3352	.	+	0	ID=BBa_K322921;Name=BBa_K322921;Parent=BBa_K917002
BBa_K917010	.	point_mutation	2126	2131	.	+	0	ID=annotation2098481;Name=Removed EcoRI site;Parent=BBa_K322921
BBa_K917010	.	CDS	1928	3349	.	+	0	ID=annotation2098480;Name=Coding sequence;Parent=BBa_K322921
BBa_K917010	.	ribosome_entry_site	1916	1918	.	+	0	ID=annotation2098459;Name=RBS;Parent=BBa_K322921
BBa_K917010	.	promoter	976	1175	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K917002
BBa_K917010	.	start_codon	1148	1148	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K917010	.	CDS	976	1063	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K917010	.	promoter	1136	1141	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K917010	.	non_covalent_binding_site	1141	1175	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K917010	.	non_covalent_binding_site	1064	1101	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K917010	.	engineered_region	976	1175	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K917010	.	promoter	1112	1117	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K917010	.	CDS	1202	1907	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K917002
BBa_K917010	.	CDS	1202	1876	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K917010	.	engineered_tag	1883	1907	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K917010
ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgca
caagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccata
ttcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcg
cgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacat
ggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccga
ccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggt
attagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcct
gtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttg
atgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccatt
ctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgt
attgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctc
cttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgct
cgatgagtttttctaataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctg
gcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccc
caggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaa
agaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttcc
gttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaag
ttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaa
acacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa
gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggta
ccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgga
agacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacc
acctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacg
aagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgcta
gtgtagatcgctactagaggagacatgaacgatgaacatcaaaaagtttgcaaaacaagcaacagtattaacctttact
accgcactgctggcaggaggcgcaactcaagcgtttgcgaaagaaacgaaccaaaagccatataaggaaacatacggca
tttcccatattacacgccatgatatgctgcaaatccctgaacagcaaaaaaatgaaaaatatcaagttcctgagttcga
ttcgtccacaattaaaaatatctcttctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctgacggc
actgtcgcaaactatcacggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacatcgattt
acatgttctatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacagcgacaa
attcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatctgacggaaaa
atccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaactgcacaagttaacgtatcag
catcagacagctctttgaacatcaacggtgtagaggattataaatcaatctttgacggtgacggaaaaacgtatcaaaa
tgtacagcagttcatcgatgaaggcaactacagctcaggcgacaaccatacgctgagagatcctcactacgtagaagat
aaaggccacaaatacttagtatttgaagcaaacactggaactgaagatggctaccaaggcgaagaatctttatttaaca
aagcatactatggcaaaagcacatcattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacgcacggc
tgagttagcaaacggcgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccgctgatt
gcatctaacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggtacctgttcactgact
cccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtttctaattctttaac
tggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcctaacgatgtaacctttacttac
tcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaagctatatgacaaacagaggattctacgcag
acaaacaatcaacgtttgcgccaagcttcctgctgaacatcaaaggcaagaaaacatctgttgtcaaagacagcatcct
tgaacaaggacaattaacagttaacaaataataa