LOCUS       BBa_K917011             2052 bp    DNA     linear       15-Jun-2024
DEFINITION  lac promoter plus sucrose hydrolase (cscA)
ACCESSION   BBa_K917011
VERSION     BBa_K917011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     misc_feature    619..621
                     /label=start codon
     misc_feature    2050..2052
                     /label=stop codon
     RBS             609..610
                     /label=RBS
     misc_feature    609..2052
                     /label=BBa_K917000
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagga tgataaaaat gacgcaatct cgattgcatg cggcgcaaaa cgcactagca
      661 aaacttcacg agcgccgagg taacactttc tatccccatt ttcacctcgc gcctcctgcc
      721 gggtggatga acgatccaaa cggcctgatc tggtttaacg atcgttatca cgcgttttat
      781 caacatcacc caatgagtga acactggggg ccaatgcact ggggacatgc caccagcgac
      841 gatatgatcc actggcagca tgagcctatt gcgctagcgc caggagacga gaatgacaaa
      901 gatgggtgtt tttcaggtag tgctgtcgat gacaatggtg tcctctcact tatctacacc
      961 ggacacgtct ggctcgatgg tgcaggtaat gacgatgcaa ttcgcgaagt acaatgtctg
     1021 gctaccagtc gggatggtat tcatttcgag aaacagggtg tgatcctcac tccaccagaa
     1081 ggaatcatgc acttccgcga tcctaaagtg tggcgtgaag ccgacacatg gtggatggta
     1141 gtcggggcga aagacccagg caacactggg cagatcctgc tttatcgcgg cagttcatta
     1201 cgtgaatgga ctttcgatcg cgtactggcc cacgctgatg cgggtgaaag ctatatgtgg
     1261 gaatgtccgg actttttcag ccttggcgat cagcattatc tgatgttttc cccgcaggga
     1321 atgaatgccg agggatacag ttaccgaaat cgctttcaaa gtggcgtaat acccggaatg
     1381 tggtcgccag gacgactttt tgcacaatcc gggcatttta ctgaacttga taacgggcat
     1441 gacttttatg caccacaaag ctttgtagcg aaggatggtc ggcgtattgt tatcggctgg
     1501 atggatatgt gggaatcgcc aatgccctca aaacgtgaag gctgggcagg ctgcatgacg
     1561 ctggcgcgcg agctatcaga gagcaatggc aaacttctac aacgcccggt acacgaagct
     1621 gagtcgttac gccagcagca tcaatctatc tctccccgca caatcagcaa taaatatgtt
     1681 ttgcaggaaa acgcgcaagc agttgagatt cagttgcagt gggcgctgaa gaacagtgat
     1741 gccgaacatt acggattaca gctcggcact ggaatgcggc tgtatattga taaccaatct
     1801 gagcgacttg ttttgtggcg gtattaccca cacgagaatt tagacggcta ccgtagtatt
     1861 cccctcccgc agcgtgacac gctcgcccta aggatattta tcgatacatc atccgtggaa
     1921 gtatttatta acgacgggga agcggtgatg agtagtcgaa tctatccgca gccagaagaa
     1981 cgggaactgt cgctttatgc ctcccacgga gtggctgtgg tgcaacatgg agcactctgg
     2041 caactgggtt aa
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