LOCUS BBa_K917013 7075 bp DNA linear 22-Nov-2024 DEFINITION pLac promoter plus cytochrome maturation gene cluster ccmA-H ACCESSION BBa_K917013 VERSION BBa_K917013.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI misc_feature 248..285 /label=CAP binding site misc_feature 332..352 /label=LacI binding site RBS 359..362 /label=rbs CDS 370..600 /label=lacZ' promoter 320..325 /label=-10 promoter 297..302 /label=-35 misc_feature 1..600 /label=BBa_J33207 RBS 633..635 /label=RBS misc_feature 639..641 /label=Start codon misc_feature 7073..7075 /label=Stop misc_feature 609..7075 /label=BBa_K917006 ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag 361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg 421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga 601 tactagagga gtgccgtaac tgtcacaact tcgagtatat ggatacaacc gcccagaaat 661 cggttgccgc gaagatgcat gaccaggcgg tgaaagatgg gcaaacctgt attgattgcc 721 ataaagggat agcgcacaag ctgcccgata tgcgtgaagt cgagccaggt ttttaacagg 781 gttattgcgt gggtatgctt gaagccagag agttactttg tgagcgggat gaacgaacct 841 tatttagtgg cttgtcattt acgctgaacg caggagagtg ggtacaaatc accggtagca 901 acggcgcggg gaagacaacg cttctccgtt tgctgacggg gttgtctcgc cctgacgcag 961 gcgaggttct ctggcaaggg cagcccttgc atcaggtacg cgacagctac catcaaaacc 1021 tgttatggat aggccatcag ccggggatca aaacccggct gacggcgtta gaaaatctgc 1081 acttttatca tcgcgatggc gataccgcac aatgtctgga agccctggcg caggccgggc 1141 ttgccggatt cgaagatatt cctgtaaatc agctctcggc cgggcaacaa cgccgcgtcg 1201 ctttagcgcg tctgtggctg acccgtgcca cgttatggat cctcgacgag ccttttaccg 1261 cgattgacgt taacggtgtc gatcgtctga cccagcgtat ggcgcagcat acggagcagg 1321 gggggattgt gattctgact acccaccagc cgctcaacgt tgctgaaagt aaaattcgcc 1381 gcatttcact gacgcaaacg agggccgcat gatgttctgg cgcattttcc gtcttgagct 1441 gcgtgtagcg tttcgccata gcgccgaaat cgccaacccg ctgtggttct tcctgattgt 1501 aattaccctt tttccgctca gtatcggtcc ggagccgcaa ctgctggcgc gtattgcacc 1561 gggcattatc tgggttgctg cgctgctttc atccttgctg gcgctggaac gactgttccg 1621 tgacgatttg caggacggca gtcttgaaca attgatgttg ttgccgttac ccttgcccgc 1681 cgttgtgctg gcgaaggtga tggcgcactg gatggtaacc ggtctgccgt tactcatcct 1741 ttcgccactg gtagcaatgc tactgggaat ggatgtttat ggctggcaag tgatggcgct 1801 gacgctgctg ctgggaacgc ctacgcttgg ctttctcggt gcaccgggcg tggcgctgac 1861 agtgggactt aagcgcggtg gtgtgctgct cagcatactg gtgttaccgc tgactatccc 1921 attactcatc tttgccaccg ccgcgatgga cgcggcttct atgcatttgc ccgttgacgg 1981 gtatctggca attttaggcg cgttgctggc aggcaccgcg acattaagtc cttttgcgac 2041 ggcggcagcg ttacgaatca gcattcaata acgctggatt actgattttt gttcgagtct 2101 ggtatcgaaa ctatgtggaa aacactgcat caactggcga tcccaccacg gctgtatcaa 2161 atctgtggct ggtttatacc gtggctggca attgccagtg tggtcgtgct taccgtcggc 2221 tggatctggg gattcggctt tgctccggct gattatcagc agggaaatag ctaccgcatt 2281 atctacctgc atgtgcctgc ggcgatctgg tcgatgggca tttatgcatc aatggcagtg 2341 gcagcgttta ttggccttgt ctggcagatg aaaatggcca acctggcggt ggcggcgatg 2401 gcccccattg gtgccgtgtt tacctttatt gccctggtta ccggctctgc atggggaaaa 2461 ccgatgtggg gcacctggtg ggtatgggat gcacgtctga cttctgaact ggtgctgctg 2521 tttttgtatg tgggtgtgat tgccctgtgg cacgccttcg acgaccgccg tctggcgggc 2581 cgtgcggcag gtatcctggt gctgattggc gtggtgaatc tgccgattat tcattactcc 2641 gtggagtggt ggaacaccct gcatcaggga tcaacgcgga tgcagcaaag tatcgatccg 2701 gcgatgcgtt cgccgctgcg ctggtcgatt tttggcttcc tgctcctgtc tgccacgctg 2761 acgctgatgc ggatgcgtaa tttgattttg ctgatggaaa aacgccgtcc gtgggtgagt 2821 gaactgatac tgaaaagagg ccgtaaatga cccctgcatt tgcttcctgg aatgaatttt 2881 tcgcaatggg cggttacgcc ttttttgtct ggctggcggt ggtgatgacc gttattccgc 2941 tggtggtttt ggtcgtgcac tcggtgatgc aacatcgcgc aattctgcgt ggcgtggcgc 3001 aacagcgggc gcgtgaggcg cgtttacgtg ctgcgcaaca gcaggaggct gcatgaatat 3061 tcgccgtaaa aaccgcttgt ggattgcctg tgccgtgttg gcagggctgg cgctgactat 3121 cggtctggtg ctatatgcgc tgcgctcgaa tatcgatctc ttttatacgc cgggggaaat 3181 tctctacggc aagcgtgaaa ctcagcaaat gccggaagtc ggtcagcgtc tgcgcgttgg 3241 cgggatggtg atgccgggta gtgtgcagcg cgatcccaat tcgctgaaag tgaccttcac 3301 catttacgat gctgaaggct cagtggatgt ctcttacgaa ggcattttgc cggatctgtt 3361 ccgtgaaggg cagggcgttg tggtgcaggg cgaactggaa aaaggcaatc atatcctcgc 3421 gaaagaagtg ctggcgaaac acgatgaaaa ctatacgccg ccagaagttg agaaagcgat 3481 ggaagctaac caccgtcgcc cggcgagtgt ttataaggac ccagcatcat gatgccagaa 3541 attggtaacg gactgctgtg cctggcgctg ggaattgcgc tgctgctgtc cgtgtatccg 3601 ctatggggcg tggcgcgcgg agatgcgcgc atgatggcgt cttcccgctt gtttgcctgg 3661 ctgctgttta tgtctgtggc tggcgcattt ctggtgctgg tcaatgcttt cgtggtcaac 3721 gacttcaccg tcacctatgt tgccagcaac tccaataccc agcttccggt gtggtatcgc 3781 gtggcggcta cctggggcgc gcatgaaggc tcgctactgc tgtgggtgct gctgatgagc 3841 ggctggacct ttgcggtggc gatttttagt cagcgtattc cgctggatat tgtggcccgt 3901 gtactggcga taatggggat ggtcagtgtc ggctttttgc tgttcattct ctttacctct 3961 aacccgttct ctcgcacgtt gccgaacttc ccgattgaag gtcgcgatct taacccgcta 4021 ttgcaggatc cggggctgat cttccatccg cctctgcttt atatggggta cgtgggtttc 4081 tcggtggcgt ttgcttttgc cattgcttct ttgctgagcg ggcgtctgga cagcacttat 4141 gcgcgtttta ctcgtccgtg gacgctggca gcgtggatct tcctgacgct cggcatcgtg 4201 ctcggttccg catgggccta ttacgaactc ggctggggtg gctggtggtt ctgggatccg 4261 gtagaaaacg cctcgtttat gccgtggctg gtggggactg cgctgatgca ctcactggcg 4321 gtcactgaac aacgcgccag cttcaaagcg tggacattac tgctggcaat cagtgccttc 4381 tcgttgtgtc tgttggggac tttcctcgtg cgttccggcg tgctggtatc ggtacacgcg 4441 tttgcgtctg atccggcgcg cggtatgttt atcctcgcct ttatggtgct ggtgattggc 4501 ggttcgctgc tgctgtttgc cgcgcgtgga cacaaagttc gctcacgcgt aaacaatgcg 4561 ctgtggtcgc gggaatcttt gctgttagcg aacaatgttt tgctggtcgc tgcgatgctg 4621 gtggtgttgc tggggacgct gctgccgttg gtgcataagc aactgggact gggcagtatt 4681 tcgattggcg aaccgttctt caacaccatg tttacctggc tgatggtgcc gtttgcgcta 4741 ctgcttggtg tcggtcctct ggtgcgctgg gggcgggatc gcccgcgtaa gatccgcaat 4801 ttattgatta tcgccttcat ctctacgctg gtgctgtcgc tgctgttgcc gtggctgttc 4861 gaaagcaaag ttgtggcgat gacggtgctc ggcctggcaa tggcctgctg gattgcggtg 4921 ctggcaattg cggaagctgc gctacgtatt tcacgcggca cgaaaaccac cttcagttat 4981 tgggggatgg tggcggctca ccttgggctg gcagtgacaa ttgttggcat tgcctttagc 5041 cagaactata gcgttgagcg tgatgtgcgc atgaagtccg gcgatagcgt cgatattcat 5101 gaatatcgct tcaccttccg tgatgtcaaa gaggtgactg gcccgaactg gcgtggcggt 5161 gtggcgacta tcggcgtaac gcgcgatggc aagccggaaa cggtgctgta tgcggaaaaa 5221 cgttattaca acactgccgg gtcgatgatg accgaagcgg caattgacgg cggcatcacg 5281 cgtgacctgt acgcggccct cggtgaagag ctggaaaacg gcgcgtgggc cgtgcgtctt 5341 tactacaaac catttgttcg ctggatttgg gcgggcgggc tgatgatggc gttgggcgga 5401 ctgctgtgtc tgtttgatcc tcgctatcgt aagcgcgtga gtccgcaaaa aactgcgccg 5461 gaggccgtat gaagcgcaaa gtattgttaa ttccgttgat tatcttcctg gcgattgccg 5521 cggcgctgct gtggcagctg gcgcgtaatg ccgaagggga tgatccgacc aatctggaat 5581 cggcgctcat tggcaagcct gtgccgaagt ttcgtctcga atcactggac aatccggggc 5641 agttttatca ggcggatgtg ctgactcagg gcaaaccagt actgcttaac gtctgggcga 5701 cctggtgtcc gacctgccgt gcggaacatc aatatctgaa tcagctttct gcgcagggca 5761 tccgcgtggt cggcatgaac tataaagacg atcgccagaa ggcaatcagc tggctgaaag 5821 agctgggcaa tccttacgcg ctaagcctgt ttgatggcga tggcatgtta gggctggatc 5881 tcggtgtcta tggcgcgcca gaaacgtttc ttattgacgg caacggcatc attcgctatc 5941 gccatgcggg cgatctgaat cctcgcgtct gggaagaaga gatcaagccg ctgtgggaga 6001 aatacagtaa ggaggccgca caatgaggtt tttattgggc gtgctgatgc tgatgatctc 6061 cggctcagcg ctggcgacca tcgatgtgtt gcagtttaaa gatgaagcac aggaacaaca 6121 gttccgtcag ctcactgaag aactgcgctg cccgaaatgc cagaacaaca gcattgccga 6181 ttccaactcg atgattgcca ccgacctgcg tcagaaagtg tatgaactga tgcaggaagg 6241 taaaagtaaa aaagagattg tcgattatat ggtggcgcgt tacggcaact tcgtcactta 6301 cgatccgccg ttaacgccgc tgaccgtgct gctgtgggtg ctgccagtag tggctattgg 6361 cattggcggt tgggtcatat acgcccgctc gcggcgtcgg gtacgcgtgg tgccggaagc 6421 gtttcctgaa caaagcgtgc cggaaggtaa gcgtgccgga tatgttgttt atctgccggg 6481 tattgtggtg gcgttaattg tggctggcgt cagctactac cagactggca attatcagca 6541 ggtgaaaatc tggcagcagg ccacggcaca ggctccggcg ttgctggaca gggcgctgga 6601 tccgaaagcc gatccgctca acgaagaaga gatgtcgcgt cttgcgctgg ggatgcgtac 6661 tcaactgcaa aaaaatccgg gagatataga aggctggatt atgttgggcc gcgttggcat 6721 ggcgctgggt aacgccagta tcgccaccga tgcatacgcc actgcgtatc gcctcgatcc 6781 gaaaaacagt gatgctgcac tgggatacgc tgaagcgttg actcgttcat ctgatcccaa 6841 cgacaaccgc ctcggcggtg aactgctacg tcagctggtg agaacggacc atagcaatat 6901 ccgtgtgcta agcatgtatg cgtttaatgc ctttgagcag cagcgatttg gcgaagccgt 6961 tgccgcgtgg gagatgatgt tgaaactctt acctgccaac gatactcgcc gtgcggtgat 7021 tgaacgtagt atcgcgcagg cgatgcaaca tttgtcgccg caggagagta aataa //