BBa_K917013 1 BBa_K917013 pLac promoter plus cytochrome maturation gene cluster ccmA-H 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z pLac gene comes from E. coli BL21 genomic DNA ccm gene cluster was cloned from E. coli JM109 This part consists of the standard registry lac promoter (BBa_J33207) plus Escherichia coli cytochrome maturation gene cluster ccmA-H with native RBS (BBa_K917006). false false _1182_ 0 13804 9 In stock true no special notes false Jakub Krakowiak component2195997 1 BBa_K917006 component2195993 1 BBa_J33207 annotation2195997 1 BBa_K917006 range2195997 1 609 7075 annotation2195993 1 BBa_J33207 range2195993 1 1 600 BBa_K917006 1 BBa_K917006 E. coli cytochrome maturation gene cluster ccmA-H 2012-08-09T11:00:00Z 2015-05-08T01:13:46Z Escherichia coli JM109 chromosomal DNA This part consists of the native Escherichia coli cytochrome maturation gene cluster formed by 8 genes and their native RBSs: ccmA, ccmB, ccmC, ccmD, ccmE, ccmF, ccmG, ccmH. The function of this cluster is to control folding and transport of cytochromes. false false _1182_ 0 13804 9 In stock true Obtained by PCR amplification of E coli JM109 gene cluster false Jakub Krakowiak annotation2191514 1 Stop range2191514 1 6465 6467 annotation2191512 1 RBS range2191512 1 25 27 annotation2191513 1 Start codon range2191513 1 31 33 BBa_J33207 1 BBa_J33207 lac promoter and lacZ 2006-10-26T11:00:00Z 2015-08-31T04:08:46Z The DNA was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575). The annotation shown here is based on that associated with this Genbank entry. The sequence shown here is derived by sequencing the construct. This part (submitted in pSB1A2) consists of the lac promoter and lacZ' gene, encoding the N-terminal 76 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation for blue-white selection on Xgal plates. A SacI site has been introduced at the 3' end, overlapping the XbaI site of the Biobrick prefix. This is designed to be used as a cloning vector for making new biobricks. PCR primers can be designed with a SacI site in one primer and an SpeI site in the other. This removes the necessity for an excessively long non-complementary tail on one primer bearing either the full biobrick prefix or suffix. The PCR product can then be digested with SacI and SpeI for insertion into this plasmid, replacing the Plac-lacZ' cassette. Recombinant plasmids will then be white on IPTG/Xgal plates, whereas any that still contain the original insert will be blue. We have used this strategy to prepare several biobricks, including BBa_J33204, which contains the xylE gene encoding catechol-2,3-dioxygenase. (For making biobricks that contain lacZ', BBa_J33204 can be used in the same way; in this case, clones with plasmids that still contain xylE will turn yellow on addition of a drop of 10 mM catechol.) false false _63_ 0 837 63 It's complicated true Note that the SacI site overlaps the SpeI site. The Biobrick prefix ends ...TCTAGAG. When this is added to the CTC at the start of the sequence shown here, the SacI site, GAGCTC, is generated. false Chris French annotation1907855 1 CAP binding site range1907855 1 248 285 annotation1907858 1 lacZ' range1907858 1 370 600 annotation1907854 1 SacI range1907854 1 1 3 annotation1907857 1 rbs range1907857 1 359 362 annotation1907860 1 -35 range1907860 1 297 302 annotation1907859 1 -10 range1907859 1 320 325 annotation1907856 1 LacI binding site range1907856 1 332 352 BBa_K917006_sequence 1 gagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa BBa_K917013_sequence 1 ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagaggagtgccgtaactgtcacaacttcgagtatatggatacaaccgcccagaaatcggttgccgcgaagatgcatgaccaggcggtgaaagatgggcaaacctgtattgattgccataaagggatagcgcacaagctgcccgatatgcgtgaagtcgagccaggtttttaacagggttattgcgtgggtatgcttgaagccagagagttactttgtgagcgggatgaacgaaccttatttagtggcttgtcatttacgctgaacgcaggagagtgggtacaaatcaccggtagcaacggcgcggggaagacaacgcttctccgtttgctgacggggttgtctcgccctgacgcaggcgaggttctctggcaagggcagcccttgcatcaggtacgcgacagctaccatcaaaacctgttatggataggccatcagccggggatcaaaacccggctgacggcgttagaaaatctgcacttttatcatcgcgatggcgataccgcacaatgtctggaagccctggcgcaggccgggcttgccggattcgaagatattcctgtaaatcagctctcggccgggcaacaacgccgcgtcgctttagcgcgtctgtggctgacccgtgccacgttatggatcctcgacgagccttttaccgcgattgacgttaacggtgtcgatcgtctgacccagcgtatggcgcagcatacggagcagggggggattgtgattctgactacccaccagccgctcaacgttgctgaaagtaaaattcgccgcatttcactgacgcaaacgagggccgcatgatgttctggcgcattttccgtcttgagctgcgtgtagcgtttcgccatagcgccgaaatcgccaacccgctgtggttcttcctgattgtaattaccctttttccgctcagtatcggtccggagccgcaactgctggcgcgtattgcaccgggcattatctgggttgctgcgctgctttcatccttgctggcgctggaacgactgttccgtgacgatttgcaggacggcagtcttgaacaattgatgttgttgccgttacccttgcccgccgttgtgctggcgaaggtgatggcgcactggatggtaaccggtctgccgttactcatcctttcgccactggtagcaatgctactgggaatggatgtttatggctggcaagtgatggcgctgacgctgctgctgggaacgcctacgcttggctttctcggtgcaccgggcgtggcgctgacagtgggacttaagcgcggtggtgtgctgctcagcatactggtgttaccgctgactatcccattactcatctttgccaccgccgcgatggacgcggcttctatgcatttgcccgttgacgggtatctggcaattttaggcgcgttgctggcaggcaccgcgacattaagtccttttgcgacggcggcagcgttacgaatcagcattcaataacgctggattactgatttttgttcgagtctggtatcgaaactatgtggaaaacactgcatcaactggcgatcccaccacggctgtatcaaatctgtggctggtttataccgtggctggcaattgccagtgtggtcgtgcttaccgtcggctggatctggggattcggctttgctccggctgattatcagcagggaaatagctaccgcattatctacctgcatgtgcctgcggcgatctggtcgatgggcatttatgcatcaatggcagtggcagcgtttattggccttgtctggcagatgaaaatggccaacctggcggtggcggcgatggcccccattggtgccgtgtttacctttattgccctggttaccggctctgcatggggaaaaccgatgtggggcacctggtgggtatgggatgcacgtctgacttctgaactggtgctgctgtttttgtatgtgggtgtgattgccctgtggcacgccttcgacgaccgccgtctggcgggccgtgcggcaggtatcctggtgctgattggcgtggtgaatctgccgattattcattactccgtggagtggtggaacaccctgcatcagggatcaacgcggatgcagcaaagtatcgatccggcgatgcgttcgccgctgcgctggtcgatttttggcttcctgctcctgtctgccacgctgacgctgatgcggatgcgtaatttgattttgctgatggaaaaacgccgtccgtgggtgagtgaactgatactgaaaagaggccgtaaatgacccctgcatttgcttcctggaatgaatttttcgcaatgggcggttacgccttttttgtctggctggcggtggtgatgaccgttattccgctggtggttttggtcgtgcactcggtgatgcaacatcgcgcaattctgcgtggcgtggcgcaacagcgggcgcgtgaggcgcgtttacgtgctgcgcaacagcaggaggctgcatgaatattcgccgtaaaaaccgcttgtggattgcctgtgccgtgttggcagggctggcgctgactatcggtctggtgctatatgcgctgcgctcgaatatcgatctcttttatacgccgggggaaattctctacggcaagcgtgaaactcagcaaatgccggaagtcggtcagcgtctgcgcgttggcgggatggtgatgccgggtagtgtgcagcgcgatcccaattcgctgaaagtgaccttcaccatttacgatgctgaaggctcagtggatgtctcttacgaaggcattttgccggatctgttccgtgaagggcagggcgttgtggtgcagggcgaactggaaaaaggcaatcatatcctcgcgaaagaagtgctggcgaaacacgatgaaaactatacgccgccagaagttgagaaagcgatggaagctaaccaccgtcgcccggcgagtgtttataaggacccagcatcatgatgccagaaattggtaacggactgctgtgcctggcgctgggaattgcgctgctgctgtccgtgtatccgctatggggcgtggcgcgcggagatgcgcgcatgatggcgtcttcccgcttgtttgcctggctgctgtttatgtctgtggctggcgcatttctggtgctggtcaatgctttcgtggtcaacgacttcaccgtcacctatgttgccagcaactccaatacccagcttccggtgtggtatcgcgtggcggctacctggggcgcgcatgaaggctcgctactgctgtgggtgctgctgatgagcggctggacctttgcggtggcgatttttagtcagcgtattccgctggatattgtggcccgtgtactggcgataatggggatggtcagtgtcggctttttgctgttcattctctttacctctaacccgttctctcgcacgttgccgaacttcccgattgaaggtcgcgatcttaacccgctattgcaggatccggggctgatcttccatccgcctctgctttatatggggtacgtgggtttctcggtggcgtttgcttttgccattgcttctttgctgagcgggcgtctggacagcacttatgcgcgttttactcgtccgtggacgctggcagcgtggatcttcctgacgctcggcatcgtgctcggttccgcatgggcctattacgaactcggctggggtggctggtggttctgggatccggtagaaaacgcctcgtttatgccgtggctggtggggactgcgctgatgcactcactggcggtcactgaacaacgcgccagcttcaaagcgtggacattactgctggcaatcagtgccttctcgttgtgtctgttggggactttcctcgtgcgttccggcgtgctggtatcggtacacgcgtttgcgtctgatccggcgcgcggtatgtttatcctcgcctttatggtgctggtgattggcggttcgctgctgctgtttgccgcgcgtggacacaaagttcgctcacgcgtaaacaatgcgctgtggtcgcgggaatctttgctgttagcgaacaatgttttgctggtcgctgcgatgctggtggtgttgctggggacgctgctgccgttggtgcataagcaactgggactgggcagtatttcgattggcgaaccgttcttcaacaccatgtttacctggctgatggtgccgtttgcgctactgcttggtgtcggtcctctggtgcgctgggggcgggatcgcccgcgtaagatccgcaatttattgattatcgccttcatctctacgctggtgctgtcgctgctgttgccgtggctgttcgaaagcaaagttgtggcgatgacggtgctcggcctggcaatggcctgctggattgcggtgctggcaattgcggaagctgcgctacgtatttcacgcggcacgaaaaccaccttcagttattgggggatggtggcggctcaccttgggctggcagtgacaattgttggcattgcctttagccagaactatagcgttgagcgtgatgtgcgcatgaagtccggcgatagcgtcgatattcatgaatatcgcttcaccttccgtgatgtcaaagaggtgactggcccgaactggcgtggcggtgtggcgactatcggcgtaacgcgcgatggcaagccggaaacggtgctgtatgcggaaaaacgttattacaacactgccgggtcgatgatgaccgaagcggcaattgacggcggcatcacgcgtgacctgtacgcggccctcggtgaagagctggaaaacggcgcgtgggccgtgcgtctttactacaaaccatttgttcgctggatttgggcgggcgggctgatgatggcgttgggcggactgctgtgtctgtttgatcctcgctatcgtaagcgcgtgagtccgcaaaaaactgcgccggaggccgtatgaagcgcaaagtattgttaattccgttgattatcttcctggcgattgccgcggcgctgctgtggcagctggcgcgtaatgccgaaggggatgatccgaccaatctggaatcggcgctcattggcaagcctgtgccgaagtttcgtctcgaatcactggacaatccggggcagttttatcaggcggatgtgctgactcagggcaaaccagtactgcttaacgtctgggcgacctggtgtccgacctgccgtgcggaacatcaatatctgaatcagctttctgcgcagggcatccgcgtggtcggcatgaactataaagacgatcgccagaaggcaatcagctggctgaaagagctgggcaatccttacgcgctaagcctgtttgatggcgatggcatgttagggctggatctcggtgtctatggcgcgccagaaacgtttcttattgacggcaacggcatcattcgctatcgccatgcgggcgatctgaatcctcgcgtctgggaagaagagatcaagccgctgtgggagaaatacagtaaggaggccgcacaatgaggtttttattgggcgtgctgatgctgatgatctccggctcagcgctggcgaccatcgatgtgttgcagtttaaagatgaagcacaggaacaacagttccgtcagctcactgaagaactgcgctgcccgaaatgccagaacaacagcattgccgattccaactcgatgattgccaccgacctgcgtcagaaagtgtatgaactgatgcaggaaggtaaaagtaaaaaagagattgtcgattatatggtggcgcgttacggcaacttcgtcacttacgatccgccgttaacgccgctgaccgtgctgctgtgggtgctgccagtagtggctattggcattggcggttgggtcatatacgcccgctcgcggcgtcgggtacgcgtggtgccggaagcgtttcctgaacaaagcgtgccggaaggtaagcgtgccggatatgttgtttatctgccgggtattgtggtggcgttaattgtggctggcgtcagctactaccagactggcaattatcagcaggtgaaaatctggcagcaggccacggcacaggctccggcgttgctggacagggcgctggatccgaaagccgatccgctcaacgaagaagagatgtcgcgtcttgcgctggggatgcgtactcaactgcaaaaaaatccgggagatatagaaggctggattatgttgggccgcgttggcatggcgctgggtaacgccagtatcgccaccgatgcatacgccactgcgtatcgcctcgatccgaaaaacagtgatgctgcactgggatacgctgaagcgttgactcgttcatctgatcccaacgacaaccgcctcggcggtgaactgctacgtcagctggtgagaacggaccatagcaatatccgtgtgctaagcatgtatgcgtttaatgcctttgagcagcagcgatttggcgaagccgttgccgcgtgggagatgatgttgaaactcttacctgccaacgatactcgccgtgcggtgattgaacgtagtatcgcgcaggcgatgcaacatttgtcgccgcaggagagtaaataa BBa_J33207_sequence 1 ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgaaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z