BBa_K925002

BBa_K925002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K925002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Paul Lockhart

Date created: 2012-09-23 11:00:00

Date modified: 2015-05-08 01:13:46

HisTagGST

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K925002_sequence (Version 1)

Description

This part produces glutathione S-transferase (GST) protein with a histidine tag attached to the end. The protein produced binds to nickel beads.

Notes

In the design of this part our major difficulty was trying to add a very small peptide sequence into the pGEX plasmid.

Source

This part was produced by biologically adding a previously chemically synthesised peice of DNA sequence known to bind nickel on to the end of the GST protein.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K925002/1

Member of these Collections

• iGEM Parts Registry