BBa_K925002 1 BBa_K925002 HisTagGST 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z This part was produced by biologically adding a previously chemically synthesised peice of DNA sequence known to bind nickel on to the end of the GST protein. This part produces glutathione S-transferase (GST) protein with a histidine tag attached to the end. The protein produced binds to nickel beads. false false _1190_ 0 12795 9 It's complicated false In the design of this part our major difficulty was trying to add a very small peptide sequence into the pGEX plasmid. false Michael Paul Lockhart BBa_K925002_sequence 1 atgattgatctgtattttgcgccgaccccgaacggccataaaattaccctgtttctggaagaagcgggcctggattatcgcctgattaaagtggatctgggcaaaggcggccagtttcgcccggaatttctgcgcattagcccgaacaacaaaattccggcgattgtggatcatagcccggcggatggcggcgaaccgctgagcctgtttgaaagcggcgcgattctgctgtatctggcggaaaaaaccggcctgtttctgagccatgaaacccgcgaacgcgcggtgaccctgcagtggctgttttggcaggtgggcggcctgggcccgatgctgggccagaaccatcattttaaccatgcggcgccgcagaccattccgtatgcgattgaacgctatcaggtggaaacccagcgcctgtatcatgtgctgaacaaacgcctggaaaacagcccgtggctgggcggcgaaaactatagcattgcggatattgcgtgctggccgtgggtgaacgcgtggacccgccagcgcattgatctggcgatgtatccggcggtgaaaaactggcatgaacgcattcgcagccgcccggcgaccggcctggcgctgctgaaagcgcagcatggcgatgaacgcagcgatagcaattccatcatcaccatcaccacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z