BBa_K925004 1 BBa_K925004 NiTagGST 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z This part was biologically synthesised. This part consists of a glutathione S-transferase (GST) protein that has been modified to include a short peptide sequence allowing it to bind to nickel. The purified protein will bind to both GST and nickel, giving a double ended tag and a mechanism by which to recover nickel from solution. false false _1190_ 0 12795 9 It's complicated false During the design of this part our main consideration was the difficulty of inserting a very small peice of DNA into the pGEX-6P-1 plasmid. This created difficulty as we could not do diagnostic digests and our vector was more likely to self-ligate. false Michael Paul Lockhart BBa_K925004_sequence 1 atgattgatctgtattttgcgccgaccccgaacggccataaaattaccctgtttctggaagaagcgggcctggattatcgcctgattaaagtggatctgggcaaaggcggccagtttcgcccggaatttctgcgcattagcccgaacaacaaaattccggcgattgtggatcatagcccggcggatggcggcgaaccgctgagcctgtttgaaagcggcgcgattctgctgtatctggcggaaaaaaccggcctgtttctgagccatgaaacccgcgaacgcgcggtgaccctgcagtggctgttttggcaggtgggcggcctgggcccgatgctgggccagaaccatcattttaaccatgcggcgccgcagaccattccgtatgcgattgaacgctatcaggtggaaacccagcgcctgtatcatgtgctgaacaaacgcctggaaaacagcccgtggctgggcggcgaaaactatagcattgcggatattgcgtgctggccgtgggtgaacgcgtggacccgccagcgcattgatctggcgatgtatccggcggtgaaaaactggcatgaacgcattcgcagccgcccggcgaccggcctggcgctgctgaaagcgcagcatggcgatgaacgcagcgatagcaattcatgtgtacaacatgcggttgcggtgaaggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z