BBa_K929100 1 BBa_K929100 Anti-EGFR scFv425 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z Plasmid pKMEF425bla The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. false false _1194_ 0 14271 9 In stock false PstI mutations originally occurring in the initial scFv425 sequence were eliminated by mutagenesis PCR. false Potsdam Bioware 2012 iGEM annotation2195202 1 Flag tag range2195202 1 1 13 annotation2195203 1 scFv425 range2195203 1 14 741 BBa_K929100_sequence 1 gactacaaagacgaagtgcaattgcagcagtctggggctgaactggtgaagcctggggcttcagtgaagttgtcctgcaaggcttccggctacaccttcaccagccactggatgcactgggtgaagcagagggctggacaaggccttgagtggatcggagagtttaatcccagcaacggccgtactaactacaatgagaaattcaagagcaaggccaccctgactgtagacaaatcctccagcacagcctacatgcaactcagcagcctgacatctgaggactctgcggtctattactgtgccagtcgggactatgattacgacggacggtactttgactactggggccaagggaccacggtcaccgtctcctcaggtggcggtggctcgggcggtggtgggtcgggtggtggcggatctgacatcgagctcacccagtctccagcaatcatgtctgcatctccaggggagaaggtcactatgacttgcagtgccagctcaagtgtaacttacatgtattggtaccagcagaagccaggatcctcccccagactcctgatttatgacacatccaacctggcttctggagtccctgttcgtttcagtggcagtgggtctgggacctcttactctctcacaatcagccgaatggaggctgaagatgctgccacttattactgccagcagtggagtagtcacatattcacgttcggctcggggacagaactggagatcaaacgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z