LOCUS       BBa_K929101             1683 bp    DNA     linear       27-Dec-2024
DEFINITION  Anti-EGFR scFv425 with TEV site, transmembrane region an eYFP
ACCESSION   BBa_K929101
VERSION     BBa_K929101.1  
FEATURES             Location/Qualifiers
     CDS             1..72
                     /label=SP
     CDS             1..72
                     /label=ErbB-1 Signal peptide
     misc_feature    1..72
                     /label=BBa_K157001
     misc_feature    81..93
                     /label=Flag tag
     CDS             94..821
                     /label=scFv425
     misc_feature    81..821
                     /label=BBa_K929100
     CDS             853..975
                     /label=GGGGSx3-BCR-Transmembrane
     misc_feature    830..937
                     /label=BBa_K157010
     misc_feature    944..1680
                     /label=BBa_E0030
     misc_feature    822..852
                     /label=TEV recognition site
ORIGIN
        1 atgagaccat ctggtactgc tggagccgca ttgctggcac ttttggctgc gctgtgccct
       61 gcaagcagag cagactacaa agacgaagtg caattgcagc agtctggggc tgaactggtg
      121 aagcctgggg cttcagtgaa gttgtcctgc aaggcttccg gctacacctt caccagccac
      181 tggatgcact gggtgaagca gagggctgga caaggccttg agtggatcgg agagtttaat
      241 cccagcaacg gccgtactaa ctacaatgag aaattcaaga gcaaggccac cctgactgta
      301 gacaaatcct ccagcacagc ctacatgcaa ctcagcagcc tgacatctga ggactctgcg
      361 gtctattact gtgccagtcg ggactatgat tacgacggac ggtactttga ctactggggc
      421 caagggacca cggtcaccgt ctcctcaggt ggcggtggct cgggcggtgg tgggtcgggt
      481 ggtggcggat ctgacatcga gctcacccag tctccagcaa tcatgtctgc atctccaggg
      541 gagaaggtca ctatgacttg cagtgccagc tcaagtgtaa cttacatgta ttggtaccag
      601 cagaagccag gatcctcccc cagactcctg atttatgaca catccaacct ggcttctgga
      661 gtccctgttc gtttcagtgg cagtgggtct gggacctctt actctctcac aatcagccga
      721 atggaggctg aagatgctgc cacttattac tgccagcagt ggagtagtca catattcacg
      781 ttcggctcgg ggacagaact ggagatcaaa cggggatctg gagagaattt atattttcaa
      841 gggggatctg gaggaggagg atctggcggc ggaggaagcg gtggcggcgg aagcggcatc
      901 atcatgatcc agaccctgct gatcatcctg ttcatcatcg tgcccatctt tctgctggga
      961 tctggagtga gcaagggcga ggagctgttc accgaggtgg tgcccatcct ggtcgagctg
     1021 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1081 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1141 accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg
     1201 aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1261 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1321 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1381 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1441 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1501 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1561 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1621 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1681 taa
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