BBa_K929101 1 BBa_K929101 Anti-EGFR scFv425 with TEV site, transmembrane region an eYFP 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z BBa_K157001, BBa_K929100, BBa_K157010, BBa_E0030 The Biobrick BBa_K929101 is an extended version of the Biobrick BBa_K929100 and is composed of the already existing human EGFR (ErbB-1) signal peptide (BBa_K157001), the scFv anti-EGFR-domain-3, a TEV recognition site (according to life technologies AcTEV TM Protease manual), the transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010) and the enhanced YFP ( BBa_E0030) reporter. The composite part was derived by PCR assembly. false false _1194_ 0 14271 9 In stock false The composite part was derived by PCR assembly. false Potsdam Bioware 2012 iGEM annotation2195276 1 TEV recognition site range2195276 1 822 852 annotation2195209 1 BBa_K157001 range2195209 1 1 72 annotation2195211 1 scFv425 range2195211 1 94 821 annotation2195215 1 BBa_E0030 range2195215 1 944 1680 annotation2195214 1 BBa_K157010 range2195214 1 830 937 annotation2195208 1 ErbB-1 Signal peptide range2195208 1 1 72 annotation2195212 1 BBa_K929100 range2195212 1 81 821 annotation2195213 1 GGGGSx3-BCR-Transmembrane range2195213 1 853 975 annotation2195207 1 SP range2195207 1 1 72 annotation2195210 1 Flag tag range2195210 1 81 93 BBa_K929101_sequence 1 atgagaccatctggtactgctggagccgcattgctggcacttttggctgcgctgtgccctgcaagcagagcagactacaaagacgaagtgcaattgcagcagtctggggctgaactggtgaagcctggggcttcagtgaagttgtcctgcaaggcttccggctacaccttcaccagccactggatgcactgggtgaagcagagggctggacaaggccttgagtggatcggagagtttaatcccagcaacggccgtactaactacaatgagaaattcaagagcaaggccaccctgactgtagacaaatcctccagcacagcctacatgcaactcagcagcctgacatctgaggactctgcggtctattactgtgccagtcgggactatgattacgacggacggtactttgactactggggccaagggaccacggtcaccgtctcctcaggtggcggtggctcgggcggtggtgggtcgggtggtggcggatctgacatcgagctcacccagtctccagcaatcatgtctgcatctccaggggagaaggtcactatgacttgcagtgccagctcaagtgtaacttacatgtattggtaccagcagaagccaggatcctcccccagactcctgatttatgacacatccaacctggcttctggagtccctgttcgtttcagtggcagtgggtctgggacctcttactctctcacaatcagccgaatggaggctgaagatgctgccacttattactgccagcagtggagtagtcacatattcacgttcggctcggggacagaactggagatcaaacggggatctggagagaatttatattttcaagggggatctggaggaggaggatctggcggcggaggaagcggtggcggcggaagcggcatcatcatgatccagaccctgctgatcatcctgttcatcatcgtgcccatctttctgctgggatctggagtgagcaagggcgaggagctgttcaccgaggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z