BBa_K929103 1 BBa_K929103 B-cell transmembrane region with mCherry and TEV site, framed by LoxP 2012-09-23T11:00:00Z 2015-05-08T01:13:46Z TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br> LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br> Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br> Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) This part represents the switchable membrane anchoring region of or antibody construct (BBa_K929107). false false _1194_ 0 14271 9 In stock false Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus). false Potsdam Bioware 2012 iGEM annotation2195268 1 LoxP site range2195268 1 22 55 annotation2195272 1 misc range2195272 1 856 890 annotation2195269 1 B-cell receptor transmembrane domaine with linker range2195269 1 58 144 annotation2195271 1 mCherry range2195271 1 145 852 annotation2195267 1 TEV recognition site range2195267 1 1 21 BBa_K929103_sequence 1 gagaatttatattttcaagggataacttcgtatagcatacattatacgaagttattaggcggcagcgacggcatcatcatgatccagaccctgctgatcatcctgtttattatcgtgcccatcttcctgctgctggacggctccatggtgtccaagggcgaagaggacaacatggccatcatcaaagagttcatgcggttcaaggtgcacatggaaggcagcgtgaacggccacgagttcgagattgagggcgagggcgaaggcagaccctatgagggaacccagaccgctaagctgaaagtgaccaagggcggacccctgcccttcgcctgggatatcctgtcccctcagtttatgtacggcagcaaggcctacgtgaagcaccccgccgacatccccgactacctgaagctgtctttcccagagggcttcaagtgggagagagtgatgaacttcgaggacggcggcgtcgtgaccgtgacccaggatagctctctccaggacggcgagtttatctacaaagtgaagctgcggggcaccaacttcccttccgacggccctgtgatgcagaaaaagacaatgggatgggaggccagctccgagcggatgtaccctgaagatggcgctctgaagggcgagatcaagcagcggctgaagctgaaggatggcggccactacgacgccgaagtgaaaaccacctacaaggccaaaaagcccgtgcagctgcctggcgcttacaatgtgaacatcaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtatgagcgggccgagggcagacattccaccggcggcatggatgagctgtacaagtgaataacttcgtatagcatacattatacgaagttatta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z