LOCUS       BBa_K929107             2019 bp    DNA     linear       26-Nov-2024
DEFINITION  Anti-GFP, IgG1 Fc with TEV site, TMD, mCherry, framed by LoxP
ACCESSION   BBa_K929107
VERSION     BBa_K929107.1  
FEATURES             Location/Qualifiers
     misc_feature    1..66
                     /label=IgG kappa signal peptide
     misc_feature    1..1116
                     /label=BBa_K929102
     CDS             67..414
                     /label=anti-GFP nanobody
     CDS             421..1116
                     /label=IgG1 Fc domain
     misc_feature    1117..2019
                     /label=BBa_K929103
     misc_feature    1123..1143
                     /label=TEV recognition site
     misc_feature    1144..1177
                     /label=LoxP site 1
     misc_feature    1180..1254
                     /label=B-cell receptor transmembrane domaine with linker
     CDS             1267..1974
                     /label=mCherry
     misc_feature    1978..2011
                     /label=misc
ORIGIN
        1 atggacatgc gggtgctggc tcagctgctg ggactgctgc tgctgtgttt tcccggagct
       61 cggtgccagg tgcagctggt ggaatctggt ggtgccctgg tgcagcctgg cggctctctg
      121 agactgtctt gtgccgcctc tggcttcccc gtgaacagat actccatgcg gtggtacaga
      181 caggcccctg gcaaagaacg cgagtgggtg gccggaatgt cctccgctgg cgacagatcc
      241 tcctacgagg actccgtgaa gggccggttc accatctctc gggacgacgc cagaaacacc
      301 gtgtacctcc agatgaactc cctgaagccc gaggacaccg ccgtgtacta ctgcaacgtg
      361 aacgtgggct tcgagtactg gggccagggc acacaagtga ccgtgtcctc taagggcgcc
      421 gagcccaagt cctgcgacaa gacccatacc tgcccccctt gtcctgcccc cgaactgctg
      481 ggaggccctt ccgtgttcct gttcccccca aagcctaagg acaccctgat gatctcccgg
      541 acccccgaag tgacctgcgt ggtggtggat gtgtcccacg aggaccctga agtgaagttc
      601 aattggtacg tggacggcgt ggaagtgcac aacgccaaga ccaagcccag agaggaacag
      661 tacaactcca cctaccgggt ggtgtccgtg ctgaccgtgc tgcatcagga ctggctgaac
      721 ggcaaagagt acaagtgcaa ggtgtccaac aaggccctgc ctgcccccat cgaaaagacc
      781 atctccaagg ccaagggcca gccccgggaa ccccaggtgt acacactgcc ccctagcagg
      841 gacgagctga ccaagaacca ggtgtccctg acctgtctcg tgaagggatt ctacccctcc
      901 gatatcgccg tggaatggga gtccaacggc cagcctgaga acaactacaa gaccaccccc
      961 cctgtgctgg actccgacgg ctcattcttc ctgtactcca agctgacagt ggacaagtcc
     1021 cggtggcagc agggcaacgt gttctcctgc tccgtgatgc acgaggccct gcacaaccac
     1081 tacacccaga agtccctgtc cctgagcccc ggcaagggct ccgagaattt atattttcaa
     1141 gggataactt cgtatagcat acattatacg aagttattag gcggcagcga cggcatcatc
     1201 atgatccaga ccctgctgat catcctgttt attatcgtgc ccatcttcct gctgctggac
     1261 ggctccatgg tgtccaaggg cgaagaggac aacatggcca tcatcaaaga gttcatgcgg
     1321 ttcaaggtgc acatggaagg cagcgtgaac ggccacgagt tcgagattga gggcgagggc
     1381 gaaggcagac cctatgaggg aacccagacc gctaagctga aagtgaccaa gggcggaccc
     1441 ctgcccttcg cctgggatat cctgtcccct cagtttatgt acggcagcaa ggcctacgtg
     1501 aagcaccccg ccgacatccc cgactacctg aagctgtctt tcccagaggg cttcaagtgg
     1561 gagagagtga tgaacttcga ggacggcggc gtcgtgaccg tgacccagga tagctctctc
     1621 caggacggcg agtttatcta caaagtgaag ctgcggggca ccaacttccc ttccgacggc
     1681 cctgtgatgc agaaaaagac aatgggatgg gaggccagct ccgagcggat gtaccctgaa
     1741 gatggcgctc tgaagggcga gatcaagcag cggctgaagc tgaaggatgg cggccactac
     1801 gacgccgaag tgaaaaccac ctacaaggcc aaaaagcccg tgcagctgcc tggcgcttac
     1861 aatgtgaaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1921 tatgagcggg ccgagggcag acattccacc ggcggcatgg atgagctgta caagtgaata
     1981 acttcgtata gcatacatta tacgaagtta ttaaagtag
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