BBa_K929107 1 BBa_K929107 Anti-GFP, IgG1 Fc with TEV site, TMD, mCherry, framed by LoxP 2012-09-23T11:00:00Z 2015-05-08T01:13:47Z BBa_K929102 and BBa_K929103 The antibody construct consists of two major building blocks represented by the actual antibody unit ( BBa_K929102) and the switchable membrane anchoring region (BBa_K929103). Both elements guarantee the eligibility and the easy handling of the construct and are optimized for expression in CHO cells. The antibody unit is represented by the human Ig kappa chain V-I region signal peptide (UniProt: P01601), the anti-GFP Nanobody (PDB: 3OGO) and the Fc region (UniProt: P01857). TEV protease recognition site, 2 LoxP sites, the B-cell receptor transmembrane domain (modified BBa_K157010) and the mcherry reporter display the switchable membrane anchoring region. false false _1194_ 0 14271 9 In stock true [1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82 [2] Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401 false Potsdam Bioware 2012 iGEM annotation2195367 1 IgG kappa signal peptide range2195367 1 1 66 annotation2195373 1 LoxP site 1 range2195373 1 1144 1177 annotation2195371 1 BBa_K929103 range2195371 1 1117 2019 annotation2195368 1 BBa_K929102 range2195368 1 1 1116 annotation2195370 1 IgG1 Fc domain range2195370 1 421 1116 annotation2195374 1 B-cell receptor transmembrane domaine with linker range2195374 1 1180 1254 annotation2195375 1 mCherry range2195375 1 1267 1974 annotation2195369 1 anti-GFP nanobody range2195369 1 67 414 annotation2195376 1 misc range2195376 1 1978 2011 annotation2195372 1 TEV recognition site range2195372 1 1123 1143 BBa_K929107_sequence 1 atggacatgcgggtgctggctcagctgctgggactgctgctgctgtgttttcccggagctcggtgccaggtgcagctggtggaatctggtggtgccctggtgcagcctggcggctctctgagactgtcttgtgccgcctctggcttccccgtgaacagatactccatgcggtggtacagacaggcccctggcaaagaacgcgagtgggtggccggaatgtcctccgctggcgacagatcctcctacgaggactccgtgaagggccggttcaccatctctcgggacgacgccagaaacaccgtgtacctccagatgaactccctgaagcccgaggacaccgccgtgtactactgcaacgtgaacgtgggcttcgagtactggggccagggcacacaagtgaccgtgtcctctaagggcgccgagcccaagtcctgcgacaagacccatacctgccccccttgtcctgcccccgaactgctgggaggcccttccgtgttcctgttccccccaaagcctaaggacaccctgatgatctcccggacccccgaagtgacctgcgtggtggtggatgtgtcccacgaggaccctgaagtgaagttcaattggtacgtggacggcgtggaagtgcacaacgccaagaccaagcccagagaggaacagtacaactccacctaccgggtggtgtccgtgctgaccgtgctgcatcaggactggctgaacggcaaagagtacaagtgcaaggtgtccaacaaggccctgcctgcccccatcgaaaagaccatctccaaggccaagggccagccccgggaaccccaggtgtacacactgccccctagcagggacgagctgaccaagaaccaggtgtccctgacctgtctcgtgaagggattctacccctccgatatcgccgtggaatgggagtccaacggccagcctgagaacaactacaagaccaccccccctgtgctggactccgacggctcattcttcctgtactccaagctgacagtggacaagtcccggtggcagcagggcaacgtgttctcctgctccgtgatgcacgaggccctgcacaaccactacacccagaagtccctgtccctgagccccggcaagggctccgagaatttatattttcaagggataacttcgtatagcatacattatacgaagttattaggcggcagcgacggcatcatcatgatccagaccctgctgatcatcctgtttattatcgtgcccatcttcctgctgctggacggctccatggtgtccaagggcgaagaggacaacatggccatcatcaaagagttcatgcggttcaaggtgcacatggaaggcagcgtgaacggccacgagttcgagattgagggcgagggcgaaggcagaccctatgagggaacccagaccgctaagctgaaagtgaccaagggcggacccctgcccttcgcctgggatatcctgtcccctcagtttatgtacggcagcaaggcctacgtgaagcaccccgccgacatccccgactacctgaagctgtctttcccagagggcttcaagtgggagagagtgatgaacttcgaggacggcggcgtcgtgaccgtgacccaggatagctctctccaggacggcgagtttatctacaaagtgaagctgcggggcaccaacttcccttccgacggccctgtgatgcagaaaaagacaatgggatgggaggccagctccgagcggatgtaccctgaagatggcgctctgaagggcgagatcaagcagcggctgaagctgaaggatggcggccactacgacgccgaagtgaaaaccacctacaaggccaaaaagcccgtgcagctgcctggcgcttacaatgtgaacatcaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtatgagcgggccgagggcagacattccaccggcggcatggatgagctgtacaagtgaataacttcgtatagcatacattatacgaagttattaaagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z