BBa_K929107
1
BBa_K929107
Anti-GFP, IgG1 Fc with TEV site, TMD, mCherry, framed by LoxP
2012-09-23T11:00:00Z
2015-05-08T01:13:47Z
BBa_K929102 and BBa_K929103
The antibody construct consists of two major building blocks represented by the actual antibody unit ( BBa_K929102) and the switchable membrane anchoring region (BBa_K929103). Both elements guarantee the eligibility and the easy handling of the construct and are optimized for expression in CHO cells. The antibody unit is represented by the human Ig kappa chain V-I region signal peptide (UniProt: P01601), the anti-GFP Nanobody (PDB: 3OGO) and the Fc region (UniProt: P01857). TEV protease recognition site, 2 LoxP sites, the B-cell receptor transmembrane domain (modified BBa_K157010) and the mcherry reporter display the switchable membrane anchoring region.
false
false
_1194_
0
14271
9
In stock
true
[1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82
[2] Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401
false
Potsdam Bioware 2012 iGEM
annotation2195367
1
IgG kappa signal peptide
range2195367
1
1
66
annotation2195373
1
LoxP site 1
range2195373
1
1144
1177
annotation2195371
1
BBa_K929103
range2195371
1
1117
2019
annotation2195368
1
BBa_K929102
range2195368
1
1
1116
annotation2195370
1
IgG1 Fc domain
range2195370
1
421
1116
annotation2195374
1
B-cell receptor transmembrane domaine with linker
range2195374
1
1180
1254
annotation2195375
1
mCherry
range2195375
1
1267
1974
annotation2195369
1
anti-GFP nanobody
range2195369
1
67
414
annotation2195376
1
misc
range2195376
1
1978
2011
annotation2195372
1
TEV recognition site
range2195372
1
1123
1143
BBa_K929107_sequence
1
atggacatgcgggtgctggctcagctgctgggactgctgctgctgtgttttcccggagctcggtgccaggtgcagctggtggaatctggtggtgccctggtgcagcctggcggctctctgagactgtcttgtgccgcctctggcttccccgtgaacagatactccatgcggtggtacagacaggcccctggcaaagaacgcgagtgggtggccggaatgtcctccgctggcgacagatcctcctacgaggactccgtgaagggccggttcaccatctctcgggacgacgccagaaacaccgtgtacctccagatgaactccctgaagcccgaggacaccgccgtgtactactgcaacgtgaacgtgggcttcgagtactggggccagggcacacaagtgaccgtgtcctctaagggcgccgagcccaagtcctgcgacaagacccatacctgccccccttgtcctgcccccgaactgctgggaggcccttccgtgttcctgttccccccaaagcctaaggacaccctgatgatctcccggacccccgaagtgacctgcgtggtggtggatgtgtcccacgaggaccctgaagtgaagttcaattggtacgtggacggcgtggaagtgcacaacgccaagaccaagcccagagaggaacagtacaactccacctaccgggtggtgtccgtgctgaccgtgctgcatcaggactggctgaacggcaaagagtacaagtgcaaggtgtccaacaaggccctgcctgcccccatcgaaaagaccatctccaaggccaagggccagccccgggaaccccaggtgtacacactgccccctagcagggacgagctgaccaagaaccaggtgtccctgacctgtctcgtgaagggattctacccctccgatatcgccgtggaatgggagtccaacggccagcctgagaacaactacaagaccaccccccctgtgctggactccgacggctcattcttcctgtactccaagctgacagtggacaagtcccggtggcagcagggcaacgtgttctcctgctccgtgatgcacgaggccctgcacaaccactacacccagaagtccctgtccctgagccccggcaagggctccgagaatttatattttcaagggataacttcgtatagcatacattatacgaagttattaggcggcagcgacggcatcatcatgatccagaccctgctgatcatcctgtttattatcgtgcccatcttcctgctgctggacggctccatggtgtccaagggcgaagaggacaacatggccatcatcaaagagttcatgcggttcaaggtgcacatggaaggcagcgtgaacggccacgagttcgagattgagggcgagggcgaaggcagaccctatgagggaacccagaccgctaagctgaaagtgaccaagggcggacccctgcccttcgcctgggatatcctgtcccctcagtttatgtacggcagcaaggcctacgtgaagcaccccgccgacatccccgactacctgaagctgtctttcccagagggcttcaagtgggagagagtgatgaacttcgaggacggcggcgtcgtgaccgtgacccaggatagctctctccaggacggcgagtttatctacaaagtgaagctgcggggcaccaacttcccttccgacggccctgtgatgcagaaaaagacaatgggatgggaggccagctccgagcggatgtaccctgaagatggcgctctgaagggcgagatcaagcagcggctgaagctgaaggatggcggccactacgacgccgaagtgaaaaccacctacaaggccaaaaagcccgtgcagctgcctggcgcttacaatgtgaacatcaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtatgagcgggccgagggcagacattccaccggcggcatggatgagctgtacaagtgaataacttcgtatagcatacattatacgaagttattaaagtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z