LOCUS       BBa_K929200             2704 bp    DNA     linear       23-Dec-2024
DEFINITION  N-terminal sortase motif linked to AAV2-VP2 with Myc-tag, kozak 
            and CMV
ACCESSION   BBa_K929200
VERSION     BBa_K929200.1  
FEATURES             Location/Qualifiers
     RBS             662..667
                     /label=Kozak sequence
     promoter        1..654
                     /label=CMV+RBS
     CDS             671..685
                     /label=N-terminal sortase motif
     misc_feature    686..715
                     /label=Myc-tag
     CDS             716..739
                     /label=Middle Linker
     CDS             746..2695
                     /label=AAV2-VP2/3
     misc_feature    2696..2701
                     /label=AgeI
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattactag
      661 agccgccatg ggaggcggtg gaggagagca gaagctgatc tcagaggagg acctgggtgg
      721 ttctggtggt ggttctggta ccggcgctcc gggaaaaaag aggccggtag agcactctcc
      781 tgtggagcca gactcctcct cgggaaccgg aaaggcgggc cagcagcctg caagaaaaag
      841 attgaatttt ggtcagactg gagacgcaga ctcagtacct gacccccagc ctctcggaca
      901 gccaccagca gccccctctg gtctgggaac taatacgatg gctacaggca gtggcgcacc
      961 aatggcagac aataacgagg gcgccgacgg agtgggtaat tcctcgggaa attggcattg
     1021 cgattccaca tggatgggcg acagagtcat caccaccagc acccgaacct gggccctgcc
     1081 cacctacaac aaccacctct acaaacaaat ttccagccaa tcaggagcct cgaacgacaa
     1141 tcactacttt ggctacagca ccccttgggg gtattttgac ttcaacagat tccactgcca
     1201 cttttcacca cgtgactggc aaagactcat caacaacaac tggggattcc gacccaagag
     1261 actcaacttc aagctcttta acattcaagt caaagaggtc acgcagaatg acggtacgac
     1321 gacgattgcc aataacctta ccagcacggt tcaggtgttt actgactcgg agtaccagct
     1381 cccgtacgtc ctcggctcgg cgcatcaagg atgcctcccg ccgttcccag cagacgtctt
     1441 catggtgcca cagtatggat acctcaccct gaacaacggg agtcaggcag taggacgctc
     1501 ttcattttac tgcctggagt actttccttc tcagatgctg cgtaccggaa acaactttac
     1561 cttcagctac acttttgagg acgttccttt ccacagcagc tacgctcaca gccagagtct
     1621 ggaccgtctc atgaatcctc tcatcgacca atatttgtat tacttgagca gaacaaacac
     1681 tccaagtggt accaccacgc agagtcgact tcagttttct caggccggag cgagtgacat
     1741 tcgggaccag tctaggaact ggcttcctgg accctgttac cgccagcagc gagtatcaaa
     1801 gacatctgcg gataacaaca acagtgaata ctcgtggact ggagctacca agtaccacct
     1861 caatggcaga gactctctgg tgaatccggg cccggccatg gcaagccaca aggacgatga
     1921 agaaaagttt tttcctcaga gcggggttct catctttggg aagcaaggct cagagaaaac
     1981 aaatgtggac attgaaaagg tcatgattac agacgaagag gaaatcagga caaccaatcc
     2041 cgtggctacg gagcagtatg gatccgtatc taccaacctc caggctggca acaccggcca
     2101 tcatcatcat catcatggcc tgagcgccca agcagctaca gctgatgtca acacacaagg
     2161 cgttcttcca ggcatggtct ggcaggacag agatgtgtac cttcaggggc ccatctgggc
     2221 aaagattcca cacacggacg gacattttca cccctctccc ctcatgggtg gattcggact
     2281 taaacaccct cctccacaga ttctcatcaa gaacaccccg gtacctgcga atccttcgac
     2341 caccttcagt gcggcaaagt ttgcttcctt catcacacag tactccacgg gacaggtcag
     2401 cgtggagatc gagtgggagc tccagaagga aaacagcaaa cgctggaatc ccgaaattca
     2461 gtacacttcc aactacaaca agtctgttaa tgtggacttt actgtggaca ctaatggcgt
     2521 gtattcagag cctcgcccca ttggcaccag atacctgact cgtaatctgt aattgcttgt
     2581 taatcaataa accgtttaat tcgtttcagt tgaactttgg tctctgcgta tttctttctt
     2641 atctagtttc catggctacg tagataagta gcatggcggg ttaatcatta actacaccgg
     2701 ttaa
//