LOCUS       BBa_K929201             2674 bp    DNA     linear       06-Dec-2025
DEFINITION  N-terminal sortase motif linked to AAV2-VP2 with kozak and CMV
ACCESSION   BBa_K929201
VERSION     BBa_K929201.1  
FEATURES             Location/Qualifiers
     promoter        1..654
                     /label=CMV+RBS
     RBS             662..667
                     /label=Kozak sequence
     CDS             671..685
                     /label=N-terminal sortase motif
     CDS             716..2665
                     /label=AAV2 - VP2/3
     CDS             686..709
                     /label=Middle linker
     misc_feature    2666..2671
                     /label=AgeI
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattactag
      661 agccgccatg ggaggcggtg gaggaggtgg ttctggtggt ggttctggta ccggcgctcc
      721 gggaaaaaag aggccggtag agcactctcc tgtggagcca gactcctcct cgggaaccgg
      781 aaaggcgggc cagcagcctg caagaaaaag attgaatttt ggtcagactg gagacgcaga
      841 ctcagtacct gacccccagc ctctcggaca gccaccagca gccccctctg gtctgggaac
      901 taatacgatg gctacaggca gtggcgcacc aatggcagac aataacgagg gcgccgacgg
      961 agtgggtaat tcctcgggaa attggcattg cgattccaca tggatgggcg acagagtcat
     1021 caccaccagc acccgaacct gggccctgcc cacctacaac aaccacctct acaaacaaat
     1081 ttccagccaa tcaggagcct cgaacgacaa tcactacttt ggctacagca ccccttgggg
     1141 gtattttgac ttcaacagat tccactgcca cttttcacca cgtgactggc aaagactcat
     1201 caacaacaac tggggattcc gacccaagag actcaacttc aagctcttta acattcaagt
     1261 caaagaggtc acgcagaatg acggtacgac gacgattgcc aataacctta ccagcacggt
     1321 tcaggtgttt actgactcgg agtaccagct cccgtacgtc ctcggctcgg cgcatcaagg
     1381 atgcctcccg ccgttcccag cagacgtctt catggtgcca cagtatggat acctcaccct
     1441 gaacaacggg agtcaggcag taggacgctc ttcattttac tgcctggagt actttccttc
     1501 tcagatgctg cgtaccggaa acaactttac cttcagctac acttttgagg acgttccttt
     1561 ccacagcagc tacgctcaca gccagagtct ggaccgtctc atgaatcctc tcatcgacca
     1621 atatttgtat tacttgagca gaacaaacac tccaagtggt accaccacgc agagtcgact
     1681 tcagttttct caggccggag cgagtgacat tcgggaccag tctaggaact ggcttcctgg
     1741 accctgttac cgccagcagc gagtatcaaa gacatctgcg gataacaaca acagtgaata
     1801 ctcgtggact ggagctacca agtaccacct caatggcaga gactctctgg tgaatccggg
     1861 cccggccatg gcaagccaca aggacgatga agaaaagttt tttcctcaga gcggggttct
     1921 catctttggg aagcaaggct cagagaaaac aaatgtggac attgaaaagg tcatgattac
     1981 agacgaagag gaaatcagga caaccaatcc cgtggctacg gagcagtatg gatccgtatc
     2041 taccaacctc caggctggca acaccggcca tcatcatcat catcatggcc tgagcgccca
     2101 agcagctaca gctgatgtca acacacaagg cgttcttcca ggcatggtct ggcaggacag
     2161 agatgtgtac cttcaggggc ccatctgggc aaagattcca cacacggacg gacattttca
     2221 cccctctccc ctcatgggtg gattcggact taaacaccct cctccacaga ttctcatcaa
     2281 gaacaccccg gtacctgcga atccttcgac caccttcagt gcggcaaagt ttgcttcctt
     2341 catcacacag tactccacgg gacaggtcag cgtggagatc gagtgggagc tccagaagga
     2401 aaacagcaaa cgctggaatc ccgaaattca gtacacttcc aactacaaca agtctgttaa
     2461 tgtggacttt actgtggaca ctaatggcgt gtattcagag cctcgcccca ttggcaccag
     2521 atacctgact cgtaatctgt aattgcttgt taatcaataa accgtttaat tcgtttcagt
     2581 tgaactttgg tctctgcgta tttctttctt atctagtttc catggctacg tagataagta
     2641 gcatggcggg ttaatcatta actacaccgg ttaa
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