LOCUS       BBa_K929202             1350 bp    DNA     linear       22-Nov-2024
DEFINITION  EGF-Receptor_3rd domain with PelB leadersequence,  His-tag and 
            C-terminal Sortase motif
ACCESSION   BBa_K929202
VERSION     BBa_K929202.1  
FEATURES             Location/Qualifiers
     promoter        1..654
                     /label=CMV+RBS
     misc_feature    664..669
                     /label=NgoMIV
     misc_feature    670..732
                     /label=PelB leadersequence
     CDS             733..1308
                     /label=EGFR 3rd domain
     misc_feature    1309..1326
                     /label=His-tag
     CDS             1327..1341
                     /label=C-terminal sortase motif
     misc_feature    1342..1347
                     /label=AgeI
     misc_feature    1281..1286
                     /label=ApaI
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg cctagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattactag
      661 atggccggca aatacctgct gccgaccgct gctgctggtc tgctgctcct cgctgcccag
      721 ccagccatgg ccaaaaaagt gtgcaacggc attggcattg gcgaatttaa agatagcctg
      781 agcattaacg cgaccaacat taaacatttt aaaaactgca ccagcattag cggcgatctg
      841 catattctgc cggtggcgtt tcgcggcgat agctttaccc ataccccgcc gctggatccg
      901 caggaactgg atattctgaa aaccgtgaaa gaaattaccg gctttctgct gattcaggcg
      961 tggccggaaa accgcaccga tctgcatgcg tttgaaaacc tggaaattat tcgcggtcgc
     1021 accaaacagc atggccagtt tagcctggcg gtggtgagcc tgaacattac cagcctgggc
     1081 ctgcgcagcc tgaaagaaat tagcgatggc gatgtgatta ttagcggcaa caaaaacctg
     1141 tgctatgcga acaccattaa ctggaaaaaa ctgtttggca ccagcggcca gaaaaccaaa
     1201 attattagca accgcggcga aaacagctgc aaagcgaccg gccaggtgtg ccatgcgctg
     1261 tgcagcccgg aaggctgctg gggcccggaa ccgcgcgatt gcgtgagcca tcatcatcat
     1321 catcatctgc cggaaaccgg caccggttaa
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