BBa_K931004 1 BBa_K931004 Thiamine Binding Protein (TBP) 2012-09-30T11:00:00Z 2015-05-08T01:13:47Z E. coli - genomic Thiamine-binding protein gene has a base pair size of 981bp and comes from the E. coli bacteria. The membrane thiamine-binding protein is optimized for E. coli and has a gene ID of 88096. Thiamine-binding protein plays a crucial role in the thiamine transport system by binding the thiamine molecule. It binds thiamine at a Kd of 170 nM when the optimum pH is at 5.0. The thiamine-binding protein also transports thiamine with a Km of 180 nM. false false _1196_ 0 4759 9 It's complicated false N/A false Jeremiah Pavelka BBa_K931000 1 BBa_K931000 Starch Binding Protein (SBP) 2012-09-26T11:00:00Z 2015-05-08T01:13:47Z R. Oryzae, genomic DNA Starch binding protein, also known as Starch binding domain, is a protein from the gene CBM 21 of the organism, R. oryzae. Its functional purpose is to aid starch breakdown by binding to starch and providing an optimal interaction with the amylytic enzymes. Its substrates are diverse among the carbohydrate family. It has a high affinity for rice starch, corn starch, sweet potato starch, tapioca starch, and also wheat starch. false false _1196_ 0 13008 9 In stock false N/A false Joe Alexander BBa_K931005 1 BBa_K931005 SBP - TBP 2012-09-28T11:00:00Z 2015-05-08T01:13:47Z The two genes come from separate organisms. The thiamine-binding protein gene comes from the genomic DNA of E. coli and the starch-binding protein gene comes from the genomic DNA of R. oryzae. The starch-binding protein gene (CBM21) and the thiamine-binding protein gene (ThiT) were placed in sequence with each other in that ThiT comes before CBM21. CBM21 comes from R. oryzae. It is part of an operon that codes for a two-domain enzyme, one of which binds starch molecules. This can be used to bind starch residues that are found in rice. ThiT comes from E.coli and plays a crucial role in the thiamine transport system by binding the thiamine molecule. The resulting protein complex from these two genes has a starch-binding domain and a thiamine binding domain that bind their specific substrates. false false _1196_ 0 13009 9 It's complicated false The two genes were placed in sequence of each other with the thiamine-binding protein gene preceding the starch-binding protein gene. The linker sequence between each element is ACTAGA. false Chris Clifford component2213062 1 BBa_K931000 component2213063 1 BBa_K931004 annotation2213062 1 BBa_K931000 range2213062 1 1 362 annotation2213063 1 BBa_K931004 range2213063 1 369 1349 BBa_K931004_sequence 1 atgctgaaaaagtacctgccgttgctgctgctgtgcaccgcgcctgttttcgccaaaccggtgctgaccgtttatacgtacgacagctttgcggcagactggggtccgggtccggtagtcaagaaagcctttgaagcggactgtaattgcgagctgaagatggttgcactggaagatggtgtgagcctgctgaaccgcctgcgcatggaaggtaagaatagcaaagcggacgtcgtcctgggcttggacaacaatctgctggatgccgcgagcaagaccggtttgttcgcgaaatcgggtgttgcagcagatgcggttaatgtgccaggcggttggaacaacgatacctttgtgccgtttgactacggctatttcgcatttgtttatgataagaacaaactgaagaacccaccgcaaagcctgaaagagctggtcgagagcgatcaaaattggcgtgtcatttatcaagacccgcgtacctctaccccgggtctgggtctgttgctgtggatgcagaaagtgtacggtgatgatgcaccgcaggcctggcaaaagctggcgaaaaagaccgtgacggtgacgaaaggttggagcgaagcgtatggcctgttcttgaaaggcgagtccgacctggttttgtcttacacgacgagccctgcctaccatattctggaagagaaaaaggacaactacgctgcggcaaatttcagcgaaggccactacctgcaagtcgaggtggctgcgcgtaccgctgcgagcaagcaaccggagctggcccagaaattcctgcaattcatggtgtccccggctttccagaatgcgatcccgactggcaattggatgtatccggtcgcgaacgtcaccctgccggctggttttgagcagctgacgaagccggccaccactctggaatttaccccggcagaggttgcggcacagcgccaggcgtggatcagcgagtggcagcgtgcggttagccgt BBa_K931000_sequence 1 gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcag BBa_K931005_sequence 1 gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcagtactagatgctgaaaaagtacctgccgttgctgctgctgtgcaccgcgcctgttttcgccaaaccggtgctgaccgtttatacgtacgacagctttgcggcagactggggtccgggtccggtagtcaagaaagcctttgaagcggactgtaattgcgagctgaagatggttgcactggaagatggtgtgagcctgctgaaccgcctgcgcatggaaggtaagaatagcaaagcggacgtcgtcctgggcttggacaacaatctgctggatgccgcgagcaagaccggtttgttcgcgaaatcgggtgttgcagcagatgcggttaatgtgccaggcggttggaacaacgatacctttgtgccgtttgactacggctatttcgcatttgtttatgataagaacaaactgaagaacccaccgcaaagcctgaaagagctggtcgagagcgatcaaaattggcgtgtcatttatcaagacccgcgtacctctaccccgggtctgggtctgttgctgtggatgcagaaagtgtacggtgatgatgcaccgcaggcctggcaaaagctggcgaaaaagaccgtgacggtgacgaaaggttggagcgaagcgtatggcctgttcttgaaaggcgagtccgacctggttttgtcttacacgacgagccctgcctaccatattctggaagagaaaaaggacaactacgctgcggcaaatttcagcgaaggccactacctgcaagtcgaggtggctgcgcgtaccgctgcgagcaagcaaccggagctggcccagaaattcctgcaattcatggtgtccccggctttccagaatgcgatcccgactggcaattggatgtatccggtcgcgaacgtcaccctgccggctggttttgagcagctgacgaagccggccaccactctggaatttaccccggcagaggttgcggcacagcgccaggcgtggatcagcgagtggcagcgtgcggttagccgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z