BBa_K931007
1
BBa_K931007
SBP - B12BP
2012-09-27T11:00:00Z
2015-05-08T01:13:47Z
This composite part comes from conjugating the gene BtuF from E. coli and CBM 21 from R. oryzae
A functional construct which contains the BtuF gene that ecodes for a B12 binding protein and a starch binding domain encoded by the gene, CBM 21. The construct is under the control of an L-arabinose promoter (BBa_K206000) and a terminator (BBa_K206000). The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli???s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively.
false
false
_1196_
0
13007
9
It's complicated
false
N/A
false
Jasmine Jiang
component2213065
1
BBa_K931001
component2213064
1
BBa_K931000
annotation2213065
1
BBa_K931001
range2213065
1
369
1166
annotation2213064
1
BBa_K931000
range2213064
1
1
362
BBa_K931000
1
BBa_K931000
Starch Binding Protein (SBP)
2012-09-26T11:00:00Z
2015-05-08T01:13:47Z
R. Oryzae, genomic DNA
Starch binding protein, also known as Starch binding domain, is a protein from the gene CBM 21 of the organism, R. oryzae. Its functional purpose is to aid starch breakdown by binding to starch and providing an optimal interaction with the amylytic enzymes. Its substrates are diverse among the carbohydrate family. It has a high affinity for rice starch, corn starch, sweet potato starch, tapioca starch, and also wheat starch.
false
false
_1196_
0
13008
9
In stock
false
N/A
false
Joe Alexander
BBa_K931001
1
BBa_K931001
Vitamin B12 Binding Protein (B12BP)
2012-09-26T11:00:00Z
2015-05-08T01:13:47Z
E. coli genomic DNA
Vitamin B12 binding protein is a Cluster A type binding protein of the diverse ABC substrate binding proteins. Specifically, it is called BtuCDF and is involved with transferring B12 into the periplasmic region of E. coli. There are two ATP binding regions, BtuD, and 20 transmembrane helices, BtuC, that are directly involved with the transport of B12 across the cell membrane, and BtuF, which binds B12.
false
false
_1196_
0
13008
9
In stock
false
N/A
false
Justin Emlen
BBa_K931007_sequence
1
gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcagtactagatggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat
BBa_K931000_sequence
1
gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcag
BBa_K931001_sequence
1
atggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z