BBa_K931007 1 BBa_K931007 SBP - B12BP 2012-09-27T11:00:00Z 2015-05-08T01:13:47Z This composite part comes from conjugating the gene BtuF from E. coli and CBM 21 from R. oryzae A functional construct which contains the BtuF gene that ecodes for a B12 binding protein and a starch binding domain encoded by the gene, CBM 21. The construct is under the control of an L-arabinose promoter (BBa_K206000) and a terminator (BBa_K206000). The BtuF domain is from the gene, BtuCDF, involved in vitamin B12 transfer into E. coli???s periplasmic region. The CBM 21 domain is part of the amylolytic enzyme family utilized by organisms involved in carbohydrate degradation. When expressed in concert, this construct bridges vitamin B12 to a starch substrate. The genes encoding for the B12 binding and starch binding domains come from the parts (BBa_K931001) and (BBa_K931000), respectively. false false _1196_ 0 13007 9 It's complicated false N/A false Jasmine Jiang component2213065 1 BBa_K931001 component2213064 1 BBa_K931000 annotation2213065 1 BBa_K931001 range2213065 1 369 1166 annotation2213064 1 BBa_K931000 range2213064 1 1 362 BBa_K931000 1 BBa_K931000 Starch Binding Protein (SBP) 2012-09-26T11:00:00Z 2015-05-08T01:13:47Z R. Oryzae, genomic DNA Starch binding protein, also known as Starch binding domain, is a protein from the gene CBM 21 of the organism, R. oryzae. Its functional purpose is to aid starch breakdown by binding to starch and providing an optimal interaction with the amylytic enzymes. Its substrates are diverse among the carbohydrate family. It has a high affinity for rice starch, corn starch, sweet potato starch, tapioca starch, and also wheat starch. false false _1196_ 0 13008 9 In stock false N/A false Joe Alexander BBa_K931001 1 BBa_K931001 Vitamin B12 Binding Protein (B12BP) 2012-09-26T11:00:00Z 2015-05-08T01:13:47Z E. coli genomic DNA Vitamin B12 binding protein is a Cluster A type binding protein of the diverse ABC substrate binding proteins. Specifically, it is called BtuCDF and is involved with transferring B12 into the periplasmic region of E. coli. There are two ATP binding regions, BtuD, and 20 transmembrane helices, BtuC, that are directly involved with the transport of B12 across the cell membrane, and BtuF, which binds B12. false false _1196_ 0 13008 9 In stock false N/A false Justin Emlen BBa_K931007_sequence 1 gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcagtactagatggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat BBa_K931000_sequence 1 gaattcgcggccgcttctagaatggcatcgattccgagcagcgcgtccgttcagctggatagctacaactatgacggtagcaccttctccggtaaaatctacgtgaagaacattgcgtatagcaagaaagtgacggtcgtttatgctgatggttctgacaattggaataacaatggtaacatcattgcggccagcttcagcggtccgatttccggcagcaattatgagtactggacgtttagcgcaagcgttaagggcatcaaagagttttacatcaagtacgaagtcagcggcaaaacctattacgacaataacaatagcgcgaactaccaagtgtctaccactagtagcggccgctgcag BBa_K931001_sequence 1 atggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z