BBa_K931004 1 BBa_K931004 Thiamine Binding Protein (TBP) 2012-09-30T11:00:00Z 2015-05-08T01:13:47Z E. coli - genomic Thiamine-binding protein gene has a base pair size of 981bp and comes from the E. coli bacteria. The membrane thiamine-binding protein is optimized for E. coli and has a gene ID of 88096. Thiamine-binding protein plays a crucial role in the thiamine transport system by binding the thiamine molecule. It binds thiamine at a Kd of 170 nM when the optimum pH is at 5.0. The thiamine-binding protein also transports thiamine with a Km of 180 nM. false false _1196_ 0 4759 9 It's complicated false N/A false Jeremiah Pavelka BBa_K931009 1 BBa_K931009 An L-Arabinose Controlled Thiamine Binding - Starch Binding Protein Fusion 2012-09-28T11:00:00Z 2015-05-08T01:13:47Z The four genes come from separate organisms. The L. Arabinose promoter (BBa_K206000) comes from the Registry of Standard Biological Parts and was originally submitted by Group: iGEM09_British_Columbia. Thiamine-binding protein gene comes from the genomic DNA of E. coli and the starch-binding protein gene comes from the genomic DNA of R. oryzae. The two terminator genes (B0010 B0012) come from the Registry of Standard Biological Parts and was originally submitted by Reshma Shetty. A starch-binding protein gene (CBM21) and a thiamine-binding protein gene (ThiT) were placed in sequence with each other in that ThiT comes before CBM21. CBM21 comes from R. oryzae. It is part of an operon that codes for a two-domain enzyme, one of which binds starch molecules. ThiT comes from E.coli and plays a crucial role in the thiamine transport system by binding the thiamine molecule. It has a Kd of 170 nM when the optimum pH is at 5.0 These two genes were placed after an L. Arabinose promoter sequence (BBa_K206000). This allows the protein to be expressed by adding the L. Arabinose chemical. Two terminator sequences (B0010 B0012) were placed at the end of the construct. The resulting gene construct is 2038 base pairs long. The protein complex from these two genes has a starch-binding domain and a thiamine binding domain which can bind their resulting substrates. true false _1196_ 0 13009 9 Discontinued false The four genes were placed in sequence of each other with the L. Arabinose promoter coming first, then the thiamine-binding protein gene preceded by the starch-binding protein gene coming next, and then the terminator sequences coming last. The linker sequence between each element is ACTAGA. false Chris Clifford component2210823 1 BBa_K931004 component2210824 1 BBa_K931001 annotation2210824 1 BBa_K931001 range2210824 1 988 1785 annotation2210823 1 BBa_K931004 range2210823 1 1 981 BBa_K931001 1 BBa_K931001 Vitamin B12 Binding Protein (B12BP) 2012-09-26T11:00:00Z 2015-05-08T01:13:47Z E. coli genomic DNA Vitamin B12 binding protein is a Cluster A type binding protein of the diverse ABC substrate binding proteins. Specifically, it is called BtuCDF and is involved with transferring B12 into the periplasmic region of E. coli. There are two ATP binding regions, BtuD, and 20 transmembrane helices, BtuC, that are directly involved with the transport of B12 across the cell membrane, and BtuF, which binds B12. false false _1196_ 0 13008 9 In stock false N/A false Justin Emlen BBa_K931004_sequence 1 atgctgaaaaagtacctgccgttgctgctgctgtgcaccgcgcctgttttcgccaaaccggtgctgaccgtttatacgtacgacagctttgcggcagactggggtccgggtccggtagtcaagaaagcctttgaagcggactgtaattgcgagctgaagatggttgcactggaagatggtgtgagcctgctgaaccgcctgcgcatggaaggtaagaatagcaaagcggacgtcgtcctgggcttggacaacaatctgctggatgccgcgagcaagaccggtttgttcgcgaaatcgggtgttgcagcagatgcggttaatgtgccaggcggttggaacaacgatacctttgtgccgtttgactacggctatttcgcatttgtttatgataagaacaaactgaagaacccaccgcaaagcctgaaagagctggtcgagagcgatcaaaattggcgtgtcatttatcaagacccgcgtacctctaccccgggtctgggtctgttgctgtggatgcagaaagtgtacggtgatgatgcaccgcaggcctggcaaaagctggcgaaaaagaccgtgacggtgacgaaaggttggagcgaagcgtatggcctgttcttgaaaggcgagtccgacctggttttgtcttacacgacgagccctgcctaccatattctggaagagaaaaaggacaactacgctgcggcaaatttcagcgaaggccactacctgcaagtcgaggtggctgcgcgtaccgctgcgagcaagcaaccggagctggcccagaaattcctgcaattcatggtgtccccggctttccagaatgcgatcccgactggcaattggatgtatccggtcgcgaacgtcaccctgccggctggttttgagcagctgacgaagccggccaccactctggaatttaccccggcagaggttgcggcacagcgccaggcgtggatcagcgagtggcagcgtgcggttagccgt BBa_K931009_sequence 1 atgctgaaaaagtacctgccgttgctgctgctgtgcaccgcgcctgttttcgccaaaccggtgctgaccgtttatacgtacgacagctttgcggcagactggggtccgggtccggtagtcaagaaagcctttgaagcggactgtaattgcgagctgaagatggttgcactggaagatggtgtgagcctgctgaaccgcctgcgcatggaaggtaagaatagcaaagcggacgtcgtcctgggcttggacaacaatctgctggatgccgcgagcaagaccggtttgttcgcgaaatcgggtgttgcagcagatgcggttaatgtgccaggcggttggaacaacgatacctttgtgccgtttgactacggctatttcgcatttgtttatgataagaacaaactgaagaacccaccgcaaagcctgaaagagctggtcgagagcgatcaaaattggcgtgtcatttatcaagacccgcgtacctctaccccgggtctgggtctgttgctgtggatgcagaaagtgtacggtgatgatgcaccgcaggcctggcaaaagctggcgaaaaagaccgtgacggtgacgaaaggttggagcgaagcgtatggcctgttcttgaaaggcgagtccgacctggttttgtcttacacgacgagccctgcctaccatattctggaagagaaaaaggacaactacgctgcggcaaatttcagcgaaggccactacctgcaagtcgaggtggctgcgcgtaccgctgcgagcaagcaaccggagctggcccagaaattcctgcaattcatggtgtccccggctttccagaatgcgatcccgactggcaattggatgtatccggtcgcgaacgtcaccctgccggctggttttgagcagctgacgaagccggccaccactctggaatttaccccggcagaggttgcggcacagcgccaggcgtggatcagcgagtggcagcgtgcggttagccgttactagatggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat BBa_K931001_sequence 1 atggcgaaaagcctgtttcgtgctttggtcgcgctgagcttcctggcccctctgtggttgaacgcagcaccgcgcgtgattactctgtccccagcgaacaccgagctggcctttgccgcaggcatcaccccggttggcgtgtctagctacagcgattatccgccgcaggctcagaaaattgagcaagtttcgacctggcaaggtatgaatctggagcgtatcgtcgccctgaaaccggacctggtcatcgcgtggcgcggtggtaacgcagagcgtcaagttgaccagctggcgtccctgggtattaaggttatgtgggtggacgcgaccagcatcgagcagattgctaatgcgttgcgccaactggctccgtggagcccgcaaccggacaaagcggaacaggcagcccagtccctgctggaccagtacgcacagctgaaggcccagtacgcggataaaccgaagaagcgcgtctttctgcaattcggcattaacccgccgtttacgtctggcaaagaaagcattcaaaatcaggtgttggaagtttgtggcggtgagaacatcttcaaggatagccgtgtgccttggccgcaggttagccgtgaacaagttctggcgcgtagcccgcaagcgatcgtcattacgggtggtccagaccaaatcccgaagatcaagcagtattggggtgagcaattgaaaattccggtgatcccgctgacgagcgattggttcgaacgtgcgagcccgcgtatcattctggcagcgcaacagctgtgcaatgcgctgagccaggtagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z